Tags

Type your tag names separated by a space and hit enter

Application of a biosensor for monitoring of ethanol.
Biosens Bioelectron. 2007 Aug 30; 23(1):121-9.BB

Abstract

An alcohol biosensor for the measurement of ethanol has been developed. It comprises an alcohol oxidase/chitosan immobilized eggshell membrane and a commercial oxygen sensor. Ethanol determination is based on the depletion of dissolved oxygen content upon exposure to ethanol solution. The decrease in oxygen level was monitored and related to the ethanol concentration. The biosensor response depends linearly on ethanol concentration between 60 microM and 0.80 mM with a detection limit of 30 microM (S/N=3) and 1 min response time. In the optimization studies of the enzyme biosensor the most suitable enzyme and chitosan amounts were found to be 1.0 mg and 0.30% (w/v), respectively. The phosphate buffer (pH 7.4, 25 mM) and room temperature (20-25 degrees C) were chosen as the optimum working conditions. In the characterization studies of the ethanol biosensor some parameters such as interference effects, operational and storage stability were studied in detail. The biosensor was also tested with various wine samples. The results of this newly developed biosensor were comparable to the results obtained by a gas chromatographic method.

Links

Publisher Full Text

Authors+Show Affiliations

Wen G
Center of Environmental Science and Engineer Research, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Zhang Y
No affiliation info available
Shuang S
No affiliation info available
Dong C
No affiliation info available
Choi MM
No affiliation info available

MeSH

Alcohol OxidoreductasesBiosensing TechniquesElectrochemistryEquipment DesignEquipment Failure AnalysisEthanolOxygenReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17561389

Citation

Wen, Guangming, et al. "Application of a Biosensor for Monitoring of Ethanol." Biosensors & Bioelectronics, vol. 23, no. 1, 2007, pp. 121-9.
Wen G, Zhang Y, Shuang S, et al. Application of a biosensor for monitoring of ethanol. Biosens Bioelectron. 2007;23(1):121-9.
Wen, G., Zhang, Y., Shuang, S., Dong, C., & Choi, M. M. (2007). Application of a biosensor for monitoring of ethanol. Biosensors & Bioelectronics, 23(1), 121-9.
Wen G, et al. Application of a Biosensor for Monitoring of Ethanol. Biosens Bioelectron. 2007 Aug 30;23(1):121-9. PubMed PMID: 17561389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of a biosensor for monitoring of ethanol. AU - Wen,Guangming, AU - Zhang,Yan, AU - Shuang,Shaomin, AU - Dong,Chuan, AU - Choi,Martin M F, Y1 - 2007/04/04/ PY - 2006/10/21/received PY - 2007/03/27/revised PY - 2007/03/29/accepted PY - 2007/6/15/pubmed PY - 2007/12/6/medline PY - 2007/6/15/entrez SP - 121 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 1 N2 - An alcohol biosensor for the measurement of ethanol has been developed. It comprises an alcohol oxidase/chitosan immobilized eggshell membrane and a commercial oxygen sensor. Ethanol determination is based on the depletion of dissolved oxygen content upon exposure to ethanol solution. The decrease in oxygen level was monitored and related to the ethanol concentration. The biosensor response depends linearly on ethanol concentration between 60 microM and 0.80 mM with a detection limit of 30 microM (S/N=3) and 1 min response time. In the optimization studies of the enzyme biosensor the most suitable enzyme and chitosan amounts were found to be 1.0 mg and 0.30% (w/v), respectively. The phosphate buffer (pH 7.4, 25 mM) and room temperature (20-25 degrees C) were chosen as the optimum working conditions. In the characterization studies of the ethanol biosensor some parameters such as interference effects, operational and storage stability were studied in detail. The biosensor was also tested with various wine samples. The results of this newly developed biosensor were comparable to the results obtained by a gas chromatographic method. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17561389/Application_of_a_biosensor_for_monitoring_of_ethanol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00192-3 DB - PRIME DP - Unbound Medicine ER -