Tags

Type your tag names separated by a space and hit enter

Failure to up-regulate gill Na+,K+-ATPase alpha-subunit isoform alpha1b may limit seawater tolerance of land-locked Arctic char (Salvelinus alpinus).

Abstract

Many populations of Arctic char (Salvelinus alpinus) are land-locked, physically separated from the ocean by natural barriers and unable to migrate to sea like anadromous populations. Previous studies which experimentally transferred land-locked Arctic char to seawater report high mortality rates due to osmoregulatory failure and an inability to up-regulate gill Na(+),K(+)-ATPase activity. This study examined the mRNA expression of two recently discovered alpha-subunit isoforms of gill Na(+)K(+)-ATPase (alpha1a and alpha1b) during seawater exposure of land-locked Arctic char. mRNA levels of these gill Na(+),K(+)-ATPasealpha-subunit isoforms were compared to Na(+),K(+)-ATPase activity and protein levels and related to osmoregulatory performance. Land-locked Arctic char were unable to regulate plasma osmolality following seawater exposure. Seawater exposure did not induce an increase in gill Na(+),K(+)-ATPase activity or protein levels. Na(+),K(+)-ATPase isoform alpha1a mRNA quickly decreased upon exposure to seawater, while isoform alpha1b levels were unchanged. These results suggest the inability of land-locked Arctic char to acclimate to seawater is due a failure to up-regulate gill Na(+),K(+)-ATPase activity which may be due to their inability to increase Na(+),K(+)-ATPase alpha1b mRNA expression.

Authors+Show Affiliations

Department Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17561424

Citation

Bystriansky, J S., et al. "Failure to Up-regulate Gill Na+,K+-ATPase Alpha-subunit Isoform Alpha1b May Limit Seawater Tolerance of Land-locked Arctic Char (Salvelinus Alpinus)." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 148, no. 2, 2007, pp. 332-8.
Bystriansky JS, Frick NT, Richards JG, et al. Failure to up-regulate gill Na+,K+-ATPase alpha-subunit isoform alpha1b may limit seawater tolerance of land-locked Arctic char (Salvelinus alpinus). Comp Biochem Physiol, Part A Mol Integr Physiol. 2007;148(2):332-8.
Bystriansky, J. S., Frick, N. T., Richards, J. G., Schulte, P. M., & Ballantyne, J. S. (2007). Failure to up-regulate gill Na+,K+-ATPase alpha-subunit isoform alpha1b may limit seawater tolerance of land-locked Arctic char (Salvelinus alpinus). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 148(2), 332-8.
Bystriansky JS, et al. Failure to Up-regulate Gill Na+,K+-ATPase Alpha-subunit Isoform Alpha1b May Limit Seawater Tolerance of Land-locked Arctic Char (Salvelinus Alpinus). Comp Biochem Physiol, Part A Mol Integr Physiol. 2007;148(2):332-8. PubMed PMID: 17561424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Failure to up-regulate gill Na+,K+-ATPase alpha-subunit isoform alpha1b may limit seawater tolerance of land-locked Arctic char (Salvelinus alpinus). AU - Bystriansky,J S, AU - Frick,N T, AU - Richards,J G, AU - Schulte,P M, AU - Ballantyne,J S, Y1 - 2007/05/18/ PY - 2006/10/09/received PY - 2007/05/09/revised PY - 2007/05/11/accepted PY - 2007/6/15/pubmed PY - 2007/10/20/medline PY - 2007/6/15/entrez SP - 332 EP - 8 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 148 IS - 2 N2 - Many populations of Arctic char (Salvelinus alpinus) are land-locked, physically separated from the ocean by natural barriers and unable to migrate to sea like anadromous populations. Previous studies which experimentally transferred land-locked Arctic char to seawater report high mortality rates due to osmoregulatory failure and an inability to up-regulate gill Na(+),K(+)-ATPase activity. This study examined the mRNA expression of two recently discovered alpha-subunit isoforms of gill Na(+)K(+)-ATPase (alpha1a and alpha1b) during seawater exposure of land-locked Arctic char. mRNA levels of these gill Na(+),K(+)-ATPasealpha-subunit isoforms were compared to Na(+),K(+)-ATPase activity and protein levels and related to osmoregulatory performance. Land-locked Arctic char were unable to regulate plasma osmolality following seawater exposure. Seawater exposure did not induce an increase in gill Na(+),K(+)-ATPase activity or protein levels. Na(+),K(+)-ATPase isoform alpha1a mRNA quickly decreased upon exposure to seawater, while isoform alpha1b levels were unchanged. These results suggest the inability of land-locked Arctic char to acclimate to seawater is due a failure to up-regulate gill Na(+),K(+)-ATPase activity which may be due to their inability to increase Na(+),K(+)-ATPase alpha1b mRNA expression. SN - 1095-6433 UR - https://www.unboundmedicine.com/medline/citation/17561424/Failure_to_up_regulate_gill_Na+K+_ATPase_alpha_subunit_isoform_alpha1b_may_limit_seawater_tolerance_of_land_locked_Arctic_char__Salvelinus_alpinus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(07)01019-7 DB - PRIME DP - Unbound Medicine ER -