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An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population.
Theor Appl Genet. 2007 Aug; 115(3):301-12.TA

Abstract

Diploid A genome species of wheat harbour immense variability for biotic stresses and productivity traits, and these could be transferred efficiently to hexaploid wheat through marker assisted selection, provided the target genes are tagged at diploid level first. Here we report an integrated molecular linkage map of A genome diploid wheat based on 93 recombinant inbred lines (RILs) derived from Triticum boeoticum x Triticum monococcum inter sub-specific cross. The parental lines were analysed with 306 simple sequence repeat (SSR) and 194 RFLP markers, including 66 bin mapped ESTs. Out of 306 SSRs tested for polymorphism, 74 (24.2%) did not show amplification (null) in both the parents. Overall, 171 (73.7%) of the 232 remaining SSR and 98 (50.5%) of the 194 RFLP markers were polymorphic. Both A and D genome specific SSR markers showed similar transferability to A genome of diploid wheat species. The 176 polymorphic markers, that were assayed on a set of 93 RILs, yielded 188 polymorphic loci and 177 of these as well as two additional morphological traits mapped on seven linkage groups with a total map length of 1,262 cM, which is longer than most of the available A genome linkage maps in diploid and hexaploid wheat. About 58 loci showed distorted segregation with majority of these mapping on chromosome 2A(m). With a few exceptions, the position and order of the markers was similar to the ones in other maps of the wheat A genome. Chromosome 1A(m) of T. monococcum and T. boeoticum showed a small paracentric inversion relative to the A genome of hexaploid wheat. The described linkage map could be useful for gene tagging, marker assisted gene introgression from diploid into hexaploid wheat as well as for map based cloning of genes from diploid A genome species and orthologous genes from hexaploid wheat.

Authors+Show Affiliations

Department Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University Ludhiana, Ludhiana, Punjab 141 004, India. kuldeep35@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17565482

Citation

Singh, Kuldeep, et al. "An Integrated Molecular Linkage Map of Diploid Wheat Based On a Triticum Boeoticum X T. Monococcum RIL Population." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 115, no. 3, 2007, pp. 301-12.
Singh K, Ghai M, Garg M, et al. An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population. Theor Appl Genet. 2007;115(3):301-12.
Singh, K., Ghai, M., Garg, M., Chhuneja, P., Kaur, P., Schnurbusch, T., Keller, B., & Dhaliwal, H. S. (2007). An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 115(3), 301-12.
Singh K, et al. An Integrated Molecular Linkage Map of Diploid Wheat Based On a Triticum Boeoticum X T. Monococcum RIL Population. Theor Appl Genet. 2007;115(3):301-12. PubMed PMID: 17565482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population. AU - Singh,Kuldeep, AU - Ghai,Meenu, AU - Garg,Monica, AU - Chhuneja,Parveen, AU - Kaur,Parminder, AU - Schnurbusch,Thorsten, AU - Keller,Beat, AU - Dhaliwal,H S, Y1 - 2007/05/25/ PY - 2006/10/06/received PY - 2007/03/24/accepted PY - 2007/6/15/pubmed PY - 2007/10/24/medline PY - 2007/6/15/entrez SP - 301 EP - 12 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 115 IS - 3 N2 - Diploid A genome species of wheat harbour immense variability for biotic stresses and productivity traits, and these could be transferred efficiently to hexaploid wheat through marker assisted selection, provided the target genes are tagged at diploid level first. Here we report an integrated molecular linkage map of A genome diploid wheat based on 93 recombinant inbred lines (RILs) derived from Triticum boeoticum x Triticum monococcum inter sub-specific cross. The parental lines were analysed with 306 simple sequence repeat (SSR) and 194 RFLP markers, including 66 bin mapped ESTs. Out of 306 SSRs tested for polymorphism, 74 (24.2%) did not show amplification (null) in both the parents. Overall, 171 (73.7%) of the 232 remaining SSR and 98 (50.5%) of the 194 RFLP markers were polymorphic. Both A and D genome specific SSR markers showed similar transferability to A genome of diploid wheat species. The 176 polymorphic markers, that were assayed on a set of 93 RILs, yielded 188 polymorphic loci and 177 of these as well as two additional morphological traits mapped on seven linkage groups with a total map length of 1,262 cM, which is longer than most of the available A genome linkage maps in diploid and hexaploid wheat. About 58 loci showed distorted segregation with majority of these mapping on chromosome 2A(m). With a few exceptions, the position and order of the markers was similar to the ones in other maps of the wheat A genome. Chromosome 1A(m) of T. monococcum and T. boeoticum showed a small paracentric inversion relative to the A genome of hexaploid wheat. The described linkage map could be useful for gene tagging, marker assisted gene introgression from diploid into hexaploid wheat as well as for map based cloning of genes from diploid A genome species and orthologous genes from hexaploid wheat. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/17565482/An_integrated_molecular_linkage_map_of_diploid_wheat_based_on_a_Triticum_boeoticum_x_T__monococcum_RIL_population_ L2 - https://dx.doi.org/10.1007/s00122-007-0543-z DB - PRIME DP - Unbound Medicine ER -