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Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet.
Plant Biotechnol J. 2007 Sep; 5(5):636-45.PB

Abstract

A genomic fatty acid elongation 1 (FAE1) clone was isolated from Crambe abyssinica. The genomic clone corresponds to a 1521-bp open reading frame, which encodes a protein of 507 amino acids. In yeast cells expression of CrFAE led to production of new very long chain monounsaturated fatty acids such as eicosenoic (20:1(delta11)) and erucic (22:1(delta13)) acids. Seed-specific expression in Arabidopsis thaliana resulted in up to a 12-fold increase in the proportion of erucic acid. On the other hand, in transgenic high-erucic Brassica carinata plants, the proportion of erucic acid was as high as 51.9% in the best transgenic line, a net increase of 40% compared to wild type. These results indicate that the CrFAE gene encodes a condensing enzyme involved in the biosynthesis of very long-chain fatty acids utilizing monounsaturated and saturated acyl substrates, with a strong capability for improving the erucic acid content.

Authors+Show Affiliations

Mietkiewska E
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8.
Brost JM
No affiliation info available
Giblin EM
No affiliation info available
Barton DL
No affiliation info available
Taylor DC
No affiliation info available

MeSH

AcetyltransferasesArabidopsisBrassicaCloning, MolecularCrambe PlantDNA, PlantErucic AcidsFatty Acid ElongasesFatty Acids, UnsaturatedGene Expression Regulation, EnzymologicMolecular Sequence DataPlant ProteinsPlants, Genetically ModifiedSaccharomyces cerevisiaeSequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17565584

Citation

Mietkiewska, Elzbieta, et al. "Cloning and Functional Characterization of the Fatty Acid Elongase 1 (FAE1) Gene From High Erucic Crambe Abyssinica Cv. Prophet." Plant Biotechnology Journal, vol. 5, no. 5, 2007, pp. 636-45.
Mietkiewska E, Brost JM, Giblin EM, et al. Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet. Plant Biotechnol J. 2007;5(5):636-45.
Mietkiewska, E., Brost, J. M., Giblin, E. M., Barton, D. L., & Taylor, D. C. (2007). Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet. Plant Biotechnology Journal, 5(5), 636-45.
Mietkiewska E, et al. Cloning and Functional Characterization of the Fatty Acid Elongase 1 (FAE1) Gene From High Erucic Crambe Abyssinica Cv. Prophet. Plant Biotechnol J. 2007;5(5):636-45. PubMed PMID: 17565584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet. AU - Mietkiewska,Elzbieta, AU - Brost,Jennifer M, AU - Giblin,E Michael, AU - Barton,Dennis L, AU - Taylor,David C, Y1 - 2007/06/12/ PY - 2007/6/15/pubmed PY - 2007/10/27/medline PY - 2007/6/15/entrez SP - 636 EP - 45 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 5 IS - 5 N2 - A genomic fatty acid elongation 1 (FAE1) clone was isolated from Crambe abyssinica. The genomic clone corresponds to a 1521-bp open reading frame, which encodes a protein of 507 amino acids. In yeast cells expression of CrFAE led to production of new very long chain monounsaturated fatty acids such as eicosenoic (20:1(delta11)) and erucic (22:1(delta13)) acids. Seed-specific expression in Arabidopsis thaliana resulted in up to a 12-fold increase in the proportion of erucic acid. On the other hand, in transgenic high-erucic Brassica carinata plants, the proportion of erucic acid was as high as 51.9% in the best transgenic line, a net increase of 40% compared to wild type. These results indicate that the CrFAE gene encodes a condensing enzyme involved in the biosynthesis of very long-chain fatty acids utilizing monounsaturated and saturated acyl substrates, with a strong capability for improving the erucic acid content. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/17565584/Cloning_and_functional_characterization_of_the_fatty_acid_elongase_1__FAE1__gene_from_high_erucic_Crambe_abyssinica_cv__Prophet_ L2 - https://doi.org/10.1111/j.1467-7652.2007.00268.x DB - PRIME DP - Unbound Medicine ER -