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Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction.
J Gastroenterol Hepatol. 2007 Jun; 22(6):885-92.JG

Abstract

BACKGROUND AND AIM

The aim of this study was to assess the protective effect of grape seed extract (GSE) against oxidative liver injury and fibrosis induced by biliary obstruction in rats.

METHODS

Wistar albino rats were divided into four groups; control (C), GSE-treated, bile duct ligated (BDL), and BDL and GSE-treated (BDL + GSE) groups. GSE was administered at a dose of 50 mg/kg a day orally for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels were determined to assess liver function and tissue damage, respectively. Tumor necrosis factor-alpha (TNF-alpha) and antioxidant capacity (AOC) were assayed in plasma samples. Liver tissues were taken for determination of the hepatic malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Production of reactive oxidants was monitored by chemiluminescence (CL) assay.

RESULTS

Serum AST, ALT, LDH and plasma TNF-alpha were elevated in the BDL group as compared to the control group and were significantly decreased with GSE treatment. Plasma AOC and hepatic GSH level, depressed by BDL, was elevated back to the control level in the GSE-treated BDL group. Increases in tissue MDA level, MPO activity and collagen content due to BDL were also attenuated by GSE treatment. Furthermore, luminol and lucigenin CL values in the BDL group increased dramatically compared to the control and were reduced by GSE treatment.

DISCUSSION

These results suggest that GSE protects the liver from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation; thus, restoration of oxidant and antioxidant status in the tissue.

Authors+Show Affiliations

Department of Fifth Surgery, Haydarpasa Numune Education and Research Hospital, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17565645

Citation

Dulundu, Ender, et al. "Grape Seed Extract Reduces Oxidative Stress and Fibrosis in Experimental Biliary Obstruction." Journal of Gastroenterology and Hepatology, vol. 22, no. 6, 2007, pp. 885-92.
Dulundu E, Ozel Y, Topaloglu U, et al. Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction. J Gastroenterol Hepatol. 2007;22(6):885-92.
Dulundu, E., Ozel, Y., Topaloglu, U., Toklu, H., Ercan, F., Gedik, N., & Sener, G. (2007). Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction. Journal of Gastroenterology and Hepatology, 22(6), 885-92.
Dulundu E, et al. Grape Seed Extract Reduces Oxidative Stress and Fibrosis in Experimental Biliary Obstruction. J Gastroenterol Hepatol. 2007;22(6):885-92. PubMed PMID: 17565645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction. AU - Dulundu,Ender, AU - Ozel,Yahya, AU - Topaloglu,Umit, AU - Toklu,Hale, AU - Ercan,Feriha, AU - Gedik,Nursal, AU - Sener,Goksel, PY - 2007/6/15/pubmed PY - 2007/9/28/medline PY - 2007/6/15/entrez SP - 885 EP - 92 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 22 IS - 6 N2 - BACKGROUND AND AIM: The aim of this study was to assess the protective effect of grape seed extract (GSE) against oxidative liver injury and fibrosis induced by biliary obstruction in rats. METHODS: Wistar albino rats were divided into four groups; control (C), GSE-treated, bile duct ligated (BDL), and BDL and GSE-treated (BDL + GSE) groups. GSE was administered at a dose of 50 mg/kg a day orally for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels were determined to assess liver function and tissue damage, respectively. Tumor necrosis factor-alpha (TNF-alpha) and antioxidant capacity (AOC) were assayed in plasma samples. Liver tissues were taken for determination of the hepatic malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Production of reactive oxidants was monitored by chemiluminescence (CL) assay. RESULTS: Serum AST, ALT, LDH and plasma TNF-alpha were elevated in the BDL group as compared to the control group and were significantly decreased with GSE treatment. Plasma AOC and hepatic GSH level, depressed by BDL, was elevated back to the control level in the GSE-treated BDL group. Increases in tissue MDA level, MPO activity and collagen content due to BDL were also attenuated by GSE treatment. Furthermore, luminol and lucigenin CL values in the BDL group increased dramatically compared to the control and were reduced by GSE treatment. DISCUSSION: These results suggest that GSE protects the liver from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation; thus, restoration of oxidant and antioxidant status in the tissue. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/17565645/Grape_seed_extract_reduces_oxidative_stress_and_fibrosis_in_experimental_biliary_obstruction_ L2 - https://doi.org/10.1111/j.1440-1746.2007.04875.x DB - PRIME DP - Unbound Medicine ER -