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Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining.
Genetics. 2007 Aug; 176(4):2003-14.G

Abstract

Microhomology-mediated end joining (MMEJ) joins DNA ends via short stretches [5-20 nucleotides (nt)] of direct repeat sequences, yielding deletions of intervening sequences. Non-homologous end joining (NHEJ) and single-strand annealing (SSA) are other error prone processes that anneal single-stranded DNA (ssDNA) via a few bases (<5 nt) or extensive direct repeat homologies (>20 nt). Although the genetic components involved in MMEJ are largely unknown, those in NHEJ and SSA are characterized in some detail. Here, we surveyed the role of NHEJ or SSA factors in joining of double-strand breaks (DSBs) with no complementary DNA ends that rely primarily on MMEJ repair. We found that MMEJ requires the nuclease activity of Mre11/Rad50/Xrs2, 3' flap removal by Rad1/Rad10, Nej1, and DNA synthesis by multiple polymerases including Pol4, Rad30, Rev3, and Pol32. The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ. Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity.

Authors+Show Affiliations

Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA. lees4@uthscsa.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17565964

Citation

Lee, Kihoon, and Sang Eun Lee. "Saccharomyces Cerevisiae Sae2- and Tel1-dependent Single-strand DNA Formation at DNA Break Promotes Microhomology-mediated End Joining." Genetics, vol. 176, no. 4, 2007, pp. 2003-14.
Lee K, Lee SE. Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining. Genetics. 2007;176(4):2003-14.
Lee, K., & Lee, S. E. (2007). Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining. Genetics, 176(4), 2003-14.
Lee K, Lee SE. Saccharomyces Cerevisiae Sae2- and Tel1-dependent Single-strand DNA Formation at DNA Break Promotes Microhomology-mediated End Joining. Genetics. 2007;176(4):2003-14. PubMed PMID: 17565964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining. AU - Lee,Kihoon, AU - Lee,Sang Eun, Y1 - 2007/06/11/ PY - 2007/6/15/pubmed PY - 2007/12/7/medline PY - 2007/6/15/entrez SP - 2003 EP - 14 JF - Genetics JO - Genetics VL - 176 IS - 4 N2 - Microhomology-mediated end joining (MMEJ) joins DNA ends via short stretches [5-20 nucleotides (nt)] of direct repeat sequences, yielding deletions of intervening sequences. Non-homologous end joining (NHEJ) and single-strand annealing (SSA) are other error prone processes that anneal single-stranded DNA (ssDNA) via a few bases (<5 nt) or extensive direct repeat homologies (>20 nt). Although the genetic components involved in MMEJ are largely unknown, those in NHEJ and SSA are characterized in some detail. Here, we surveyed the role of NHEJ or SSA factors in joining of double-strand breaks (DSBs) with no complementary DNA ends that rely primarily on MMEJ repair. We found that MMEJ requires the nuclease activity of Mre11/Rad50/Xrs2, 3' flap removal by Rad1/Rad10, Nej1, and DNA synthesis by multiple polymerases including Pol4, Rad30, Rev3, and Pol32. The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ. Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/17565964/Saccharomyces_cerevisiae_Sae2__and_Tel1_dependent_single_strand_DNA_formation_at_DNA_break_promotes_microhomology_mediated_end_joining_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&amp;pmid=17565964 DB - PRIME DP - Unbound Medicine ER -