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Plasmonic detection of a model analyte in serum by a gold nanorod sensor.
Anal Chem. 2007 Jul 15; 79(14):5278-83.AC

Abstract

We describe the fabrication of a label-free, chip-based biosensor based on the localized surface plasmon resonance (LSPR) of gold nanorods. Gold nanorods were chemisorbed onto a mercaptosilane-modified glass substrate, followed by conjugation of biotin to the nanorods. Streptavidin binding to biotin was monitored by the wavelength shift of the LSPR peak in the UV-vis extinction spectrum of the immobilized gold nanorods due to the change in local refractive index at the gold nanorod surface induced by streptavidin binding. The limit of detection of the sensor is 0.005 microg/mL (94 pM) in PBS and 1 microg/mL (19 nM) in serum, and the dynamic range spans 94 pM to 0.19 microM. The advantages of the nanorod-based sensor over an LSPR sensor that we had previously fabricated from gold nanospheres (Nath, N.; Chilkoti, A. Anal. Chem. 2002, 74, 504-509; J. Fluoresc. 2004, 14, 377-389; Anal. Chem. 2004, 76, 5370-5378) are the significantly lower detection limit and the internal self-reference that the signal of the nanorod sensor provides based on the measurement of peak wavelength shift.

Authors+Show Affiliations

Department of Biomedical Engineering, Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

17567106

Citation

Marinakos, Stella M., et al. "Plasmonic Detection of a Model Analyte in Serum By a Gold Nanorod Sensor." Analytical Chemistry, vol. 79, no. 14, 2007, pp. 5278-83.
Marinakos SM, Chen S, Chilkoti A. Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Anal Chem. 2007;79(14):5278-83.
Marinakos, S. M., Chen, S., & Chilkoti, A. (2007). Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical Chemistry, 79(14), 5278-83.
Marinakos SM, Chen S, Chilkoti A. Plasmonic Detection of a Model Analyte in Serum By a Gold Nanorod Sensor. Anal Chem. 2007 Jul 15;79(14):5278-83. PubMed PMID: 17567106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmonic detection of a model analyte in serum by a gold nanorod sensor. AU - Marinakos,Stella M, AU - Chen,Sihai, AU - Chilkoti,Ashutosh, Y1 - 2007/06/14/ PY - 2007/6/15/pubmed PY - 2007/9/18/medline PY - 2007/6/15/entrez SP - 5278 EP - 83 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 14 N2 - We describe the fabrication of a label-free, chip-based biosensor based on the localized surface plasmon resonance (LSPR) of gold nanorods. Gold nanorods were chemisorbed onto a mercaptosilane-modified glass substrate, followed by conjugation of biotin to the nanorods. Streptavidin binding to biotin was monitored by the wavelength shift of the LSPR peak in the UV-vis extinction spectrum of the immobilized gold nanorods due to the change in local refractive index at the gold nanorod surface induced by streptavidin binding. The limit of detection of the sensor is 0.005 microg/mL (94 pM) in PBS and 1 microg/mL (19 nM) in serum, and the dynamic range spans 94 pM to 0.19 microM. The advantages of the nanorod-based sensor over an LSPR sensor that we had previously fabricated from gold nanospheres (Nath, N.; Chilkoti, A. Anal. Chem. 2002, 74, 504-509; J. Fluoresc. 2004, 14, 377-389; Anal. Chem. 2004, 76, 5370-5378) are the significantly lower detection limit and the internal self-reference that the signal of the nanorod sensor provides based on the measurement of peak wavelength shift. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17567106/Plasmonic_detection_of_a_model_analyte_in_serum_by_a_gold_nanorod_sensor_ L2 - https://doi.org/10.1021/ac0706527 DB - PRIME DP - Unbound Medicine ER -