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Cross-reactive T-cell responses to the nonstructural regions of dengue viruses among dengue fever and dengue hemorrhagic fever patients in Malaysia.
Clin Vaccine Immunol. 2007 Aug; 14(8):969-77.CV

Abstract

Dengue virus infections are a major cause of morbidity and mortality in tropical and subtropical areas in the world. Attempts to develop effective vaccines have been hampered by the lack of understanding of the pathogenesis of the disease and the absence of suitable experimental models for dengue viral infection. The magnitude of T-cell responses has been reported to correlate with dengue disease severity. Sixty Malaysian adults with dengue viral infections were investigated for their dengue virus-specific T-cell responses to 32 peptides antigens from the structural and nonstructural regions from a dengue virus isolate. Seventeen different peptides from the C, E, NS2B, NS3, NS4A, NS4B, and NS5 regions were found to evoke significant responses in a gamma interferon enzyme-linked immunospot (ELISPOT) assay of samples from 13 selected patients with dengue fever (DF) and dengue hemorrhagic fever (DHF). NS3 and predominantly NS3(422-431) were found to be important T-cell targets. The highest peaks of T-cell responses observed were in responses to NS3(422-431) and NS5(563-571) in DHF patients. We also found almost a sevenfold increase in T-cell response in three DHF patients compared to three DF patient responses to peptide NS3(422-431). A large number of patients' T cells also responded to the NS2B(97-106) region. The ELISPOT analyses also revealed high frequencies of T cells that recognize both serotype-specific and cross-reactive dengue virus antigens in patients with DHF.

Authors+Show Affiliations

Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17567768

Citation

Appanna, Ramapraba, et al. "Cross-reactive T-cell Responses to the Nonstructural Regions of Dengue Viruses Among Dengue Fever and Dengue Hemorrhagic Fever Patients in Malaysia." Clinical and Vaccine Immunology : CVI, vol. 14, no. 8, 2007, pp. 969-77.
Appanna R, Huat TL, See LL, et al. Cross-reactive T-cell responses to the nonstructural regions of dengue viruses among dengue fever and dengue hemorrhagic fever patients in Malaysia. Clin Vaccine Immunol. 2007;14(8):969-77.
Appanna, R., Huat, T. L., See, L. L., Tan, P. L., Vadivelu, J., & Devi, S. (2007). Cross-reactive T-cell responses to the nonstructural regions of dengue viruses among dengue fever and dengue hemorrhagic fever patients in Malaysia. Clinical and Vaccine Immunology : CVI, 14(8), 969-77.
Appanna R, et al. Cross-reactive T-cell Responses to the Nonstructural Regions of Dengue Viruses Among Dengue Fever and Dengue Hemorrhagic Fever Patients in Malaysia. Clin Vaccine Immunol. 2007;14(8):969-77. PubMed PMID: 17567768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-reactive T-cell responses to the nonstructural regions of dengue viruses among dengue fever and dengue hemorrhagic fever patients in Malaysia. AU - Appanna,Ramapraba, AU - Huat,Tan Lian, AU - See,Lucy Lum Chai, AU - Tan,Phoay Lay, AU - Vadivelu,Jamuna, AU - Devi,Shamala, Y1 - 2007/06/13/ PY - 2007/6/15/pubmed PY - 2007/10/16/medline PY - 2007/6/15/entrez SP - 969 EP - 77 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 14 IS - 8 N2 - Dengue virus infections are a major cause of morbidity and mortality in tropical and subtropical areas in the world. Attempts to develop effective vaccines have been hampered by the lack of understanding of the pathogenesis of the disease and the absence of suitable experimental models for dengue viral infection. The magnitude of T-cell responses has been reported to correlate with dengue disease severity. Sixty Malaysian adults with dengue viral infections were investigated for their dengue virus-specific T-cell responses to 32 peptides antigens from the structural and nonstructural regions from a dengue virus isolate. Seventeen different peptides from the C, E, NS2B, NS3, NS4A, NS4B, and NS5 regions were found to evoke significant responses in a gamma interferon enzyme-linked immunospot (ELISPOT) assay of samples from 13 selected patients with dengue fever (DF) and dengue hemorrhagic fever (DHF). NS3 and predominantly NS3(422-431) were found to be important T-cell targets. The highest peaks of T-cell responses observed were in responses to NS3(422-431) and NS5(563-571) in DHF patients. We also found almost a sevenfold increase in T-cell response in three DHF patients compared to three DF patient responses to peptide NS3(422-431). A large number of patients' T cells also responded to the NS2B(97-106) region. The ELISPOT analyses also revealed high frequencies of T cells that recognize both serotype-specific and cross-reactive dengue virus antigens in patients with DHF. SN - 1556-6811 UR - https://www.unboundmedicine.com/medline/citation/17567768/Cross_reactive_T_cell_responses_to_the_nonstructural_regions_of_dengue_viruses_among_dengue_fever_and_dengue_hemorrhagic_fever_patients_in_Malaysia_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=17567768 DB - PRIME DP - Unbound Medicine ER -