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Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron.
EMBO J 2007; 26(13):3157-68EJ

Abstract

Iron homeostasis requires subtle control systems, as iron is both essential and toxic. In Aspergillus nidulans, iron represses iron acquisition via the GATA factor SreA, and induces iron-dependent pathways at the transcriptional level, by a so far unknown mechanism. Here, we demonstrate that iron-dependent pathways (e.g., heme biosynthesis) are repressed during iron-depleted conditions by physical interaction of HapX with the CCAAT-binding core complex (CBC). Proteome analysis identified putative HapX targets. Mutual transcriptional control between hapX and sreA and synthetic lethality resulting from deletion of both regulatory genes indicate a tight interplay of these control systems. Expression of genes encoding CBC subunits was not influenced by iron availability, and their deletion was deleterious during iron-depleted and iron-replete conditions. Expression of hapX was repressed by iron and its deletion was deleterious during iron-depleted conditions only. These data indicate that the CBC has a general role and that HapX function is confined to iron-depleted conditions. Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae.

Authors+Show Affiliations

Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), and Friedrich-Schiller-University Jena, Jena, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17568774

Citation

Hortschansky, Peter, et al. "Interaction of HapX With the CCAAT-binding Complex--a Novel Mechanism of Gene Regulation By Iron." The EMBO Journal, vol. 26, no. 13, 2007, pp. 3157-68.
Hortschansky P, Eisendle M, Al-Abdallah Q, et al. Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron. EMBO J. 2007;26(13):3157-68.
Hortschansky, P., Eisendle, M., Al-Abdallah, Q., Schmidt, A. D., Bergmann, S., Thön, M., ... Haas, H. (2007). Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron. The EMBO Journal, 26(13), pp. 3157-68.
Hortschansky P, et al. Interaction of HapX With the CCAAT-binding Complex--a Novel Mechanism of Gene Regulation By Iron. EMBO J. 2007 Jul 11;26(13):3157-68. PubMed PMID: 17568774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron. AU - Hortschansky,Peter, AU - Eisendle,Martin, AU - Al-Abdallah,Qusai, AU - Schmidt,André D, AU - Bergmann,Sebastian, AU - Thön,Marcel, AU - Kniemeyer,Olaf, AU - Abt,Beate, AU - Seeber,Birgit, AU - Werner,Ernst R, AU - Kato,Masashi, AU - Brakhage,Axel A, AU - Haas,Hubertus, Y1 - 2007/06/14/ PY - 2006/08/17/received PY - 2007/05/16/accepted PY - 2007/6/15/pubmed PY - 2007/12/6/medline PY - 2007/6/15/entrez SP - 3157 EP - 68 JF - The EMBO journal JO - EMBO J. VL - 26 IS - 13 N2 - Iron homeostasis requires subtle control systems, as iron is both essential and toxic. In Aspergillus nidulans, iron represses iron acquisition via the GATA factor SreA, and induces iron-dependent pathways at the transcriptional level, by a so far unknown mechanism. Here, we demonstrate that iron-dependent pathways (e.g., heme biosynthesis) are repressed during iron-depleted conditions by physical interaction of HapX with the CCAAT-binding core complex (CBC). Proteome analysis identified putative HapX targets. Mutual transcriptional control between hapX and sreA and synthetic lethality resulting from deletion of both regulatory genes indicate a tight interplay of these control systems. Expression of genes encoding CBC subunits was not influenced by iron availability, and their deletion was deleterious during iron-depleted and iron-replete conditions. Expression of hapX was repressed by iron and its deletion was deleterious during iron-depleted conditions only. These data indicate that the CBC has a general role and that HapX function is confined to iron-depleted conditions. Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/17568774/Interaction_of_HapX_with_the_CCAAT_binding_complex__a_novel_mechanism_of_gene_regulation_by_iron_ L2 - http://emboj.embopress.org/cgi/pmidlookup?view=long&pmid=17568774 DB - PRIME DP - Unbound Medicine ER -