Tags

Type your tag names separated by a space and hit enter

Analysis of a chlorosulfolipid from Ochromonas danica by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(14):2188-94.RC

Abstract

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry (MS) is an established tool for analyzing high mass molecules, such as proteins, whereas it attracts far less interest in the field of lipid analysis. In the study reported here a new chlorosulfolipid (CSL), 3,8,12,15-tetrachloroeicosane-1,17,18-triyl tris(hydrogen sulfate), was identified from the alga Ochromonas danica and de novo characterized by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-ToF) MS in negative ion mode. This method provides an effective alternative for the analysis of compounds directly derived from organic cell extracts. For MALDI analyses several frequently used solid MALDI matrices as well as some ionic liquid matrices (ILMs) were tested to enhance the analyte response to UV-laser and its ionization. The molecular weight of the observed compound could be determined as Li-, Na- and K-adducts [M+Me-2H]-. The characteristic isotopic patterns of the measured ions and the well-allocated molecular fragments by MS1, MS2 and MS3 indicate the fourfold chlorination and threefold sulfation of the investigated compound. The MS fragmentation alongside of the chlorine-bearing C-atoms is accompanied by the generation of a double bond at the opposite fragment in MS1. This obtained fragmentation pattern provides an insight into the allocation of the chlorine-bearing C-atoms along the carbon chain.

Authors+Show Affiliations

Institute of Bioprocess Engineering, Friedrich-Alexander-University Erlangen - Nuremberg 91052 Erlangen, Germany. Kai.Darsow@bvt.cbi.uni-erlangen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17569098

Citation

Darsow, Kai H., et al. "Analysis of a Chlorosulfolipid From Ochromonas Danica By Matrix-assisted Laser Desorption/ionization Quadrupole Ion Trap Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 14, 2007, pp. 2188-94.
Darsow KH, Lange HA, Resch M, et al. Analysis of a chlorosulfolipid from Ochromonas danica by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(14):2188-94.
Darsow, K. H., Lange, H. A., Resch, M., Walter, C., & Buchholz, R. (2007). Analysis of a chlorosulfolipid from Ochromonas danica by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(14), 2188-94.
Darsow KH, et al. Analysis of a Chlorosulfolipid From Ochromonas Danica By Matrix-assisted Laser Desorption/ionization Quadrupole Ion Trap Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(14):2188-94. PubMed PMID: 17569098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of a chlorosulfolipid from Ochromonas danica by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. AU - Darsow,Kai H, AU - Lange,Harald A, AU - Resch,Martin, AU - Walter,Christian, AU - Buchholz,Rainer, PY - 2007/6/15/pubmed PY - 2007/8/24/medline PY - 2007/6/15/entrez SP - 2188 EP - 94 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 14 N2 - Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry (MS) is an established tool for analyzing high mass molecules, such as proteins, whereas it attracts far less interest in the field of lipid analysis. In the study reported here a new chlorosulfolipid (CSL), 3,8,12,15-tetrachloroeicosane-1,17,18-triyl tris(hydrogen sulfate), was identified from the alga Ochromonas danica and de novo characterized by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-ToF) MS in negative ion mode. This method provides an effective alternative for the analysis of compounds directly derived from organic cell extracts. For MALDI analyses several frequently used solid MALDI matrices as well as some ionic liquid matrices (ILMs) were tested to enhance the analyte response to UV-laser and its ionization. The molecular weight of the observed compound could be determined as Li-, Na- and K-adducts [M+Me-2H]-. The characteristic isotopic patterns of the measured ions and the well-allocated molecular fragments by MS1, MS2 and MS3 indicate the fourfold chlorination and threefold sulfation of the investigated compound. The MS fragmentation alongside of the chlorine-bearing C-atoms is accompanied by the generation of a double bond at the opposite fragment in MS1. This obtained fragmentation pattern provides an insight into the allocation of the chlorine-bearing C-atoms along the carbon chain. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17569098/Analysis_of_a_chlorosulfolipid_from_Ochromonas_danica_by_matrix_assisted_laser_desorption/ionization_quadrupole_ion_trap_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -