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Studies on direct electron transfer and biocatalytic properties of hemoglobin in polyacrylonitrile matrix.
Bioelectrochemistry. 2007 Nov; 71(2):198-203.B

Abstract

The direct electrochemistry of hemoglobin (Hb) immobilized in polyacrylonitrile (PAN) modified glassy carbon electrode was described. The protein-PAN film exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks for Hb Fe(III)/Fe(II) redox couple in a pH 7.0 phosphate buffer. The formal potential of Hb heme Fe(III)/Fe(II) couple varied linearly with the increase of pH in the range of 5.0-9.0 with a slope of 54 mV pH(-1), which implied that a proton transfer was accompanied with each electron transfer in the electrochemical reaction. Position of Soret absorption band of Hb-PAN film suggested that the Hb kept its secondary structure similar to its native state in the PAN matrix. The Hb in PAN matrix acted as a biologic catalyst to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H(2)O(2) concentration ranging from 8.3 x 10(-6) to 5 x 10(-4) mol L(-1) with a detection limit of 8.3 x 10(-6) mol L(-1) at 3 sigma. The apparent Michaelis-Menten constant K(M)(app) for H(2)O(2) sensor was estimated to be 0.9 mmol L(-1).

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17569598

Citation

Shan, Dan, et al. "Studies On Direct Electron Transfer and Biocatalytic Properties of Hemoglobin in Polyacrylonitrile Matrix." Bioelectrochemistry (Amsterdam, Netherlands), vol. 71, no. 2, 2007, pp. 198-203.
Shan D, Wang S, Zhu D, et al. Studies on direct electron transfer and biocatalytic properties of hemoglobin in polyacrylonitrile matrix. Bioelectrochemistry. 2007;71(2):198-203.
Shan, D., Wang, S., Zhu, D., & Xue, H. (2007). Studies on direct electron transfer and biocatalytic properties of hemoglobin in polyacrylonitrile matrix. Bioelectrochemistry (Amsterdam, Netherlands), 71(2), 198-203.
Shan D, et al. Studies On Direct Electron Transfer and Biocatalytic Properties of Hemoglobin in Polyacrylonitrile Matrix. Bioelectrochemistry. 2007;71(2):198-203. PubMed PMID: 17569598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on direct electron transfer and biocatalytic properties of hemoglobin in polyacrylonitrile matrix. AU - Shan,Dan, AU - Wang,Shanxia, AU - Zhu,Daobin, AU - Xue,Huaiguo, Y1 - 2007/05/21/ PY - 2006/09/24/received PY - 2007/04/17/revised PY - 2007/04/30/accepted PY - 2007/6/16/pubmed PY - 2008/2/27/medline PY - 2007/6/16/entrez SP - 198 EP - 203 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 71 IS - 2 N2 - The direct electrochemistry of hemoglobin (Hb) immobilized in polyacrylonitrile (PAN) modified glassy carbon electrode was described. The protein-PAN film exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks for Hb Fe(III)/Fe(II) redox couple in a pH 7.0 phosphate buffer. The formal potential of Hb heme Fe(III)/Fe(II) couple varied linearly with the increase of pH in the range of 5.0-9.0 with a slope of 54 mV pH(-1), which implied that a proton transfer was accompanied with each electron transfer in the electrochemical reaction. Position of Soret absorption band of Hb-PAN film suggested that the Hb kept its secondary structure similar to its native state in the PAN matrix. The Hb in PAN matrix acted as a biologic catalyst to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H(2)O(2) concentration ranging from 8.3 x 10(-6) to 5 x 10(-4) mol L(-1) with a detection limit of 8.3 x 10(-6) mol L(-1) at 3 sigma. The apparent Michaelis-Menten constant K(M)(app) for H(2)O(2) sensor was estimated to be 0.9 mmol L(-1). SN - 1567-5394 UR - https://www.unboundmedicine.com/medline/citation/17569598/Studies_on_direct_electron_transfer_and_biocatalytic_properties_of_hemoglobin_in_polyacrylonitrile_matrix_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(07)00040-0 DB - PRIME DP - Unbound Medicine ER -