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Alpha3beta4-nicotinic receptors mediate adrenergic nerve- and peptidergic (CGRP) nerve-dependent vasodilation induced by nicotine in rat mesenteric arteries.
Br J Pharmacol. 2007 Aug; 151(8):1216-23.BJ

Abstract

BACKGROUND AND PURPOSE

Previous studies demonstrated that nicotine-induced endothelium-independent vasodilation is mediated by perivascular adrenergic nerves and nerves releasing calcitonin gene-related peptide (CGRPergic nerves). We characterized the nicotinic acetylcholine (ACh) receptor subtype underlying the vasodilation in response to nicotine in rat mesenteric arteries.

EXPERIMENTAL APPROACH

Rat mesenteric vascular beds without endothelium were contracted by perfusion with Krebs solution containing methoxamine and the perfusion pressure was measured with a pressure transducer.

KEY RESULTS

Perfusion of nicotine (1-100 microM) for 1 min caused a concentration-dependent decrease in perfusion pressure due to vasodilation. Perfusion of (+/-)-epibatidine (1-100 nM) (non-selective agonist) or (-)-cytisine (1-100 microM) (partial agonist for nicotinic beta2 subtype and full agonist for nicotinic beta4 subtype) induced vasodilation in a concentration-dependent manner. Vasodilation induced by nicotine, (-)-cytisine- and (+/-)-epibatidine was markedly attenuated by guanethidine (5 microM) and pretreatment with capsaicin (1 microM). Mecamylamine (relatively selective antagonist for alpha3beta4 subtype), but not dihydro-beta-erythroidine (selective antagonist for alpha4beta2 subtype) or alpha-bungarotoxin (selective antagonist for alpha7 subtype), markedly inhibited nicotine-induced vasodilation. Nicotine-induced vasodilation was inhibited by methyllycaconitine at high concentrations (>1 microM), which non-selectively antagonize nicotinic receptors, while a low concentration of 10 nM, which selectively antagonizes alpha7 subtype, had no effect. (-)-Cytisine and (+/-)-epibatidine-induced vasodilation were abolished by mecamylamine.

CONCLUSION AND IMPLICATIONS

These results suggest that the nicotinic alpha3beta4 receptor subtype, but not the alpha7 and alpha4beta2 subtypes, is responsible for the vasodilation in rat mesenteric arteries induced by nicotine- and nicotinic ACh receptor agonists through stimulation of adrenergic and CGRPergic perivascular nerves.

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17572697

Citation

Eguchi, S, et al. "Alpha3beta4-nicotinic Receptors Mediate Adrenergic Nerve- and Peptidergic (CGRP) Nerve-dependent Vasodilation Induced By Nicotine in Rat Mesenteric Arteries." British Journal of Pharmacology, vol. 151, no. 8, 2007, pp. 1216-23.
Eguchi S, Miyashita S, Kitamura Y, et al. Alpha3beta4-nicotinic receptors mediate adrenergic nerve- and peptidergic (CGRP) nerve-dependent vasodilation induced by nicotine in rat mesenteric arteries. Br J Pharmacol. 2007;151(8):1216-23.
Eguchi, S., Miyashita, S., Kitamura, Y., & Kawasaki, H. (2007). Alpha3beta4-nicotinic receptors mediate adrenergic nerve- and peptidergic (CGRP) nerve-dependent vasodilation induced by nicotine in rat mesenteric arteries. British Journal of Pharmacology, 151(8), 1216-23.
Eguchi S, et al. Alpha3beta4-nicotinic Receptors Mediate Adrenergic Nerve- and Peptidergic (CGRP) Nerve-dependent Vasodilation Induced By Nicotine in Rat Mesenteric Arteries. Br J Pharmacol. 2007;151(8):1216-23. PubMed PMID: 17572697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alpha3beta4-nicotinic receptors mediate adrenergic nerve- and peptidergic (CGRP) nerve-dependent vasodilation induced by nicotine in rat mesenteric arteries. AU - Eguchi,S, AU - Miyashita,S, AU - Kitamura,Y, AU - Kawasaki,H, Y1 - 2007/06/18/ PY - 2007/6/19/pubmed PY - 2007/11/1/medline PY - 2007/6/19/entrez SP - 1216 EP - 23 JF - British journal of pharmacology JO - Br J Pharmacol VL - 151 IS - 8 N2 - BACKGROUND AND PURPOSE: Previous studies demonstrated that nicotine-induced endothelium-independent vasodilation is mediated by perivascular adrenergic nerves and nerves releasing calcitonin gene-related peptide (CGRPergic nerves). We characterized the nicotinic acetylcholine (ACh) receptor subtype underlying the vasodilation in response to nicotine in rat mesenteric arteries. EXPERIMENTAL APPROACH: Rat mesenteric vascular beds without endothelium were contracted by perfusion with Krebs solution containing methoxamine and the perfusion pressure was measured with a pressure transducer. KEY RESULTS: Perfusion of nicotine (1-100 microM) for 1 min caused a concentration-dependent decrease in perfusion pressure due to vasodilation. Perfusion of (+/-)-epibatidine (1-100 nM) (non-selective agonist) or (-)-cytisine (1-100 microM) (partial agonist for nicotinic beta2 subtype and full agonist for nicotinic beta4 subtype) induced vasodilation in a concentration-dependent manner. Vasodilation induced by nicotine, (-)-cytisine- and (+/-)-epibatidine was markedly attenuated by guanethidine (5 microM) and pretreatment with capsaicin (1 microM). Mecamylamine (relatively selective antagonist for alpha3beta4 subtype), but not dihydro-beta-erythroidine (selective antagonist for alpha4beta2 subtype) or alpha-bungarotoxin (selective antagonist for alpha7 subtype), markedly inhibited nicotine-induced vasodilation. Nicotine-induced vasodilation was inhibited by methyllycaconitine at high concentrations (>1 microM), which non-selectively antagonize nicotinic receptors, while a low concentration of 10 nM, which selectively antagonizes alpha7 subtype, had no effect. (-)-Cytisine and (+/-)-epibatidine-induced vasodilation were abolished by mecamylamine. CONCLUSION AND IMPLICATIONS: These results suggest that the nicotinic alpha3beta4 receptor subtype, but not the alpha7 and alpha4beta2 subtypes, is responsible for the vasodilation in rat mesenteric arteries induced by nicotine- and nicotinic ACh receptor agonists through stimulation of adrenergic and CGRPergic perivascular nerves. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/17572697/Alpha3beta4_nicotinic_receptors_mediate_adrenergic_nerve__and_peptidergic__CGRP__nerve_dependent_vasodilation_induced_by_nicotine_in_rat_mesenteric_arteries_ L2 - https://doi.org/10.1038/sj.bjp.0707331 DB - PRIME DP - Unbound Medicine ER -