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Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma.
Respir Med. 2007 Sep; 101(9):1947-53.RM

Abstract

BACKGROUND

Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time.

METHODS

Biomarkers in serum and BAL fluid from asthma (n=13) and COPD (n=25) patients were measured using commercially available ELISA kits.

RESULTS

We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P<0.05). In both groups, BAL IL-8 correlated with SLPI (r=0.577, P<0.01 and r=0.589, P<0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r=0.576, P<0.01), while in asthma patients BAL sICAM-1 correlated with FEV(1)/FVC (r=0.418, P<0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P<0.01) and serum sICAM-1 negatively correlated with FEV(1)/FVC (r=-0.582, P<0.05).

CONCLUSION

Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma.

Authors+Show Affiliations

Department of Otolaryngology and Head and Neck Surgery, University Hospital, 20502 Malmö, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17574828

Citation

Hollander, Camilla, et al. "Serum and Bronchial Lavage Fluid Concentrations of IL-8, SLPI, sCD14 and sICAM-1 in Patients With COPD and Asthma." Respiratory Medicine, vol. 101, no. 9, 2007, pp. 1947-53.
Hollander C, Sitkauskiene B, Sakalauskas R, et al. Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. Respir Med. 2007;101(9):1947-53.
Hollander, C., Sitkauskiene, B., Sakalauskas, R., Westin, U., & Janciauskiene, S. M. (2007). Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. Respiratory Medicine, 101(9), 1947-53.
Hollander C, et al. Serum and Bronchial Lavage Fluid Concentrations of IL-8, SLPI, sCD14 and sICAM-1 in Patients With COPD and Asthma. Respir Med. 2007;101(9):1947-53. PubMed PMID: 17574828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. AU - Hollander,Camilla, AU - Sitkauskiene,Brigita, AU - Sakalauskas,Raimundas, AU - Westin,Ulla, AU - Janciauskiene,Sabina M, Y1 - 2007/06/15/ PY - 2006/11/16/received PY - 2007/04/10/revised PY - 2007/04/13/accepted PY - 2007/6/19/pubmed PY - 2007/11/10/medline PY - 2007/6/19/entrez SP - 1947 EP - 53 JF - Respiratory medicine JO - Respir Med VL - 101 IS - 9 N2 - BACKGROUND: Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time. METHODS: Biomarkers in serum and BAL fluid from asthma (n=13) and COPD (n=25) patients were measured using commercially available ELISA kits. RESULTS: We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P<0.05). In both groups, BAL IL-8 correlated with SLPI (r=0.577, P<0.01 and r=0.589, P<0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r=0.576, P<0.01), while in asthma patients BAL sICAM-1 correlated with FEV(1)/FVC (r=0.418, P<0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P<0.01) and serum sICAM-1 negatively correlated with FEV(1)/FVC (r=-0.582, P<0.05). CONCLUSION: Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/17574828/Serum_and_bronchial_lavage_fluid_concentrations_of_IL_8_SLPI_sCD14_and_sICAM_1_in_patients_with_COPD_and_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(07)00151-5 DB - PRIME DP - Unbound Medicine ER -