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In vitro benzo[a]pyrene diol epoxide-induced DNA adducts and risk of squamous cell carcinoma of head and neck.
Cancer Res. 2007 Jun 15; 67(12):5628-34.CR

Abstract

In this large confirmatory study of 803 patients with squamous cell carcinoma of head and neck (SCCHN) and 839 controls frequency matched by age, sex, and ethnicity, we further examined potential predictors of benzo[a]pyrene diol epoxide (BPDE)-induced adduct levels and their associations with SCCHN risk. BPDE-DNA adduct levels were determined by the (32)P-postlabeling method in peripheral lymphocytes after in vitro challenged by BPDE. We also genotyped for GSTM1 null, GSTT1 null, GSTP1 Ile(105)Val, and GSTP1 Ala(114)Val. Potential predictors of BPDE-DNA adducts were evaluated by stratification and multivariate linear regression analyses and the association between adduct levels and SCCHN risk by multivariate logistic regression analyses. We found that mean BPDE-DNA adduct levels (the relative adduct labeling x 10(7) +/- SD) were significantly higher in cases (77.6 +/- 111.8) than in controls (57.3 +/- 98.3; P < 0.001). Using the median control value (29.22) as a cutoff, 63% of the cases were distributed above this level (adjusted odds ratio, 1.71; 95% confidence interval, 1.39-2.10). A significant dose-response relationship was observed between adduct quartiles and SCCHN risk (P(trend) < 0.001). Multivariate linear regression analysis revealed that ethnicity and smoking were significant predictors of BPDE-DNA adduct levels in controls. In conclusion, we confirmed the previously reported association between in vitro BPDE-induced DNA adduct levels and SCCHN risk, and the assay may help identify individuals at high risk of developing smoking-related cancers.

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Authors+Show Affiliations

Li D
Department of Gastrointestinal Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Wang LE
No affiliation info available
Chang P
No affiliation info available
El-Naggar AK
No affiliation info available
Sturgis EM
No affiliation info available
Wei Q
No affiliation info available

MeSH

AgedAlcohol DrinkingBenzopyrenesCarcinogensCarcinoma, Squamous CellCase-Control StudiesDNA AdductsFemaleGenotypeGlutathione S-Transferase piGlutathione TransferaseHead and Neck NeoplasmsHumansIn Vitro TechniquesMaleMiddle AgedRisk FactorsSmoking

Pub Type(s)

Clinical Trial
Journal Article
Research Support, N.I.H., Extramural
Validation Study

Language

eng

PubMed ID

17575128

Citation

Li, Donghui, et al. "In Vitro Benzo[a]pyrene Diol Epoxide-induced DNA Adducts and Risk of Squamous Cell Carcinoma of Head and Neck." Cancer Research, vol. 67, no. 12, 2007, pp. 5628-34.
Li D, Wang LE, Chang P, et al. In vitro benzo[a]pyrene diol epoxide-induced DNA adducts and risk of squamous cell carcinoma of head and neck. Cancer Res. 2007;67(12):5628-34.
Li, D., Wang, L. E., Chang, P., El-Naggar, A. K., Sturgis, E. M., & Wei, Q. (2007). In vitro benzo[a]pyrene diol epoxide-induced DNA adducts and risk of squamous cell carcinoma of head and neck. Cancer Research, 67(12), 5628-34.
Li D, et al. In Vitro Benzo[a]pyrene Diol Epoxide-induced DNA Adducts and Risk of Squamous Cell Carcinoma of Head and Neck. Cancer Res. 2007 Jun 15;67(12):5628-34. PubMed PMID: 17575128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro benzo[a]pyrene diol epoxide-induced DNA adducts and risk of squamous cell carcinoma of head and neck. AU - Li,Donghui, AU - Wang,Li-E, AU - Chang,Ping, AU - El-Naggar,Adel K, AU - Sturgis,Erich M, AU - Wei,Qingyi, PY - 2007/6/19/pubmed PY - 2007/9/26/medline PY - 2007/6/19/entrez SP - 5628 EP - 34 JF - Cancer research JO - Cancer Res VL - 67 IS - 12 N2 - In this large confirmatory study of 803 patients with squamous cell carcinoma of head and neck (SCCHN) and 839 controls frequency matched by age, sex, and ethnicity, we further examined potential predictors of benzo[a]pyrene diol epoxide (BPDE)-induced adduct levels and their associations with SCCHN risk. BPDE-DNA adduct levels were determined by the (32)P-postlabeling method in peripheral lymphocytes after in vitro challenged by BPDE. We also genotyped for GSTM1 null, GSTT1 null, GSTP1 Ile(105)Val, and GSTP1 Ala(114)Val. Potential predictors of BPDE-DNA adducts were evaluated by stratification and multivariate linear regression analyses and the association between adduct levels and SCCHN risk by multivariate logistic regression analyses. We found that mean BPDE-DNA adduct levels (the relative adduct labeling x 10(7) +/- SD) were significantly higher in cases (77.6 +/- 111.8) than in controls (57.3 +/- 98.3; P < 0.001). Using the median control value (29.22) as a cutoff, 63% of the cases were distributed above this level (adjusted odds ratio, 1.71; 95% confidence interval, 1.39-2.10). A significant dose-response relationship was observed between adduct quartiles and SCCHN risk (P(trend) < 0.001). Multivariate linear regression analysis revealed that ethnicity and smoking were significant predictors of BPDE-DNA adduct levels in controls. In conclusion, we confirmed the previously reported association between in vitro BPDE-induced DNA adduct levels and SCCHN risk, and the assay may help identify individuals at high risk of developing smoking-related cancers. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/17575128/In_vitro_benzo[a]pyrene_diol_epoxide_induced_DNA_adducts_and_risk_of_squamous_cell_carcinoma_of_head_and_neck_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=17575128 DB - PRIME DP - Unbound Medicine ER -