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Effect of syndecan-1, syndecan-4, and glypican-1 on turkey muscle satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2.
Poult Sci. 2007 Jul; 86(7):1406-13.PS

Abstract

The membrane-associated heparan sulfate proteoglycan families consisting of the syndecans and glypicans are low-affinity receptors for fibroblast growth factor 2 (FGF2). Fibroblast growth factor 2 is a potent stimulator of skeletal muscle cell proliferation and a strong inhibitor of differentiation. Because syndecan-1, syndecan-4, and glypican-1 potentially play unique, but pivotal, roles in muscle cell proliferation and differentiation, these proteoglycans were examined for their effect on muscle cell proliferation and differentiation and FGF2 responsiveness. In the present study, turkey Randombred Control 2 line myogenic satellite cells were transfected with expression vector constructions of syndecan-1, syndecan-4, or glypican-1 to assay their role during muscle development and the effect on FGF2 responsiveness. During proliferation, only syndecan-1 increased proliferation. Both syndecan-4 and glypican-1 decreased proliferation at 72 h but generally did not affect the proliferation process. There was no interaction between the transfected gene and cell proliferation response to FGF2. Glypican-1 increased differentiation early in the process (24 h), and at later times differentiation was decreased by glypican-1. Both syndecan-1 and syndecan-4 overexpression decreased differentiation. During differentiation, except for glypican-1 at 48 h of differentiation, there was no interaction between gene treatment and FGF2 responsiveness. This result indicates that FGF2 responsiveness was not affected by the overexpression of syndecan-1, syndecan-4, and glypican-1 during differentiation. These data demonstrate that syndecan-1, syndecan-4, or glypican-1 differentially affect the processes of turkey muscle cell proliferation and differentiation, and can regulate these developmental stages in an FGF2-independent manner.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA. USA. velleman.1@osu.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17575189

Citation

Velleman, S G., et al. "Effect of Syndecan-1, Syndecan-4, and Glypican-1 On Turkey Muscle Satellite Cell Proliferation, Differentiation, and Responsiveness to Fibroblast Growth Factor 2." Poultry Science, vol. 86, no. 7, 2007, pp. 1406-13.
Velleman SG, Coy CS, McFarland DC. Effect of syndecan-1, syndecan-4, and glypican-1 on turkey muscle satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2. Poult Sci. 2007;86(7):1406-13.
Velleman, S. G., Coy, C. S., & McFarland, D. C. (2007). Effect of syndecan-1, syndecan-4, and glypican-1 on turkey muscle satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2. Poultry Science, 86(7), 1406-13.
Velleman SG, Coy CS, McFarland DC. Effect of Syndecan-1, Syndecan-4, and Glypican-1 On Turkey Muscle Satellite Cell Proliferation, Differentiation, and Responsiveness to Fibroblast Growth Factor 2. Poult Sci. 2007;86(7):1406-13. PubMed PMID: 17575189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of syndecan-1, syndecan-4, and glypican-1 on turkey muscle satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2. AU - Velleman,S G, AU - Coy,C S, AU - McFarland,D C, PY - 2007/6/19/pubmed PY - 2007/8/30/medline PY - 2007/6/19/entrez SP - 1406 EP - 13 JF - Poultry science JO - Poult Sci VL - 86 IS - 7 N2 - The membrane-associated heparan sulfate proteoglycan families consisting of the syndecans and glypicans are low-affinity receptors for fibroblast growth factor 2 (FGF2). Fibroblast growth factor 2 is a potent stimulator of skeletal muscle cell proliferation and a strong inhibitor of differentiation. Because syndecan-1, syndecan-4, and glypican-1 potentially play unique, but pivotal, roles in muscle cell proliferation and differentiation, these proteoglycans were examined for their effect on muscle cell proliferation and differentiation and FGF2 responsiveness. In the present study, turkey Randombred Control 2 line myogenic satellite cells were transfected with expression vector constructions of syndecan-1, syndecan-4, or glypican-1 to assay their role during muscle development and the effect on FGF2 responsiveness. During proliferation, only syndecan-1 increased proliferation. Both syndecan-4 and glypican-1 decreased proliferation at 72 h but generally did not affect the proliferation process. There was no interaction between the transfected gene and cell proliferation response to FGF2. Glypican-1 increased differentiation early in the process (24 h), and at later times differentiation was decreased by glypican-1. Both syndecan-1 and syndecan-4 overexpression decreased differentiation. During differentiation, except for glypican-1 at 48 h of differentiation, there was no interaction between gene treatment and FGF2 responsiveness. This result indicates that FGF2 responsiveness was not affected by the overexpression of syndecan-1, syndecan-4, and glypican-1 during differentiation. These data demonstrate that syndecan-1, syndecan-4, or glypican-1 differentially affect the processes of turkey muscle cell proliferation and differentiation, and can regulate these developmental stages in an FGF2-independent manner. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/17575189/Effect_of_syndecan_1_syndecan_4_and_glypican_1_on_turkey_muscle_satellite_cell_proliferation_differentiation_and_responsiveness_to_fibroblast_growth_factor_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)40354-4 DB - PRIME DP - Unbound Medicine ER -