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Ordered expression of virulence genes in Salmonella enterica serovar typhimurium.
Folia Microbiol (Praha). 2007; 52(2):107-14.FM

Abstract

Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes of Salmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB and sopE2) were active in Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related to S. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB and pipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity of phoP and ssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes.

Authors+Show Affiliations

Veterinary Research Institute, 621 00 Brno, Czechia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17575908

Citation

Papezova, K, et al. "Ordered Expression of Virulence Genes in Salmonella Enterica Serovar Typhimurium." Folia Microbiologica, vol. 52, no. 2, 2007, pp. 107-14.
Papezova K, Gregorova D, Jonuschies J, et al. Ordered expression of virulence genes in Salmonella enterica serovar typhimurium. Folia Microbiol (Praha). 2007;52(2):107-14.
Papezova, K., Gregorova, D., Jonuschies, J., & Rychlik, I. (2007). Ordered expression of virulence genes in Salmonella enterica serovar typhimurium. Folia Microbiologica, 52(2), 107-14.
Papezova K, et al. Ordered Expression of Virulence Genes in Salmonella Enterica Serovar Typhimurium. Folia Microbiol (Praha). 2007;52(2):107-14. PubMed PMID: 17575908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ordered expression of virulence genes in Salmonella enterica serovar typhimurium. AU - Papezova,K, AU - Gregorova,D, AU - Jonuschies,J, AU - Rychlik,I, PY - 2007/6/20/pubmed PY - 2007/7/13/medline PY - 2007/6/20/entrez SP - 107 EP - 14 JF - Folia microbiologica JO - Folia Microbiol (Praha) VL - 52 IS - 2 N2 - Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes of Salmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB and sopE2) were active in Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related to S. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB and pipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity of phoP and ssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes. SN - 0015-5632 UR - https://www.unboundmedicine.com/medline/citation/17575908/Ordered_expression_of_virulence_genes_in_Salmonella_enterica_serovar_typhimurium_ DB - PRIME DP - Unbound Medicine ER -