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Antibacterial activity of plant extracts from northwestern Argentina.
J Appl Microbiol 2007; 102(6):1450-61JA

Abstract

AIMS

To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity.

METHODS AND RESULTS

Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD(50)) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 microg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 microg ml(-1)) against Acinetobacterfreundii (303). These data were lower than their LD(50). Tripodanthus acutifolius tincture showed lower MIC (110 microg of EM per ml) and minimal bactericidal concentration (MBC) (220 microg of EM per ml) than cefotaxim (MIC and MBC of 320 microg ml(-1)) for Pseudomonasaeruginosa. This extract also showed a MIC/MBC of 110/220 microg of EM per ml, lower than oxacillin (MIC/MBC of 160/220 microg ml(-1)) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3',4',5,7-pentahydroxyflavone 3-beta-rhamnosilglucoside), iso-quercitrin (3,3',4',5,7-pentahydroxyflavone 3-beta-glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion.

CONCLUSIONS

Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina.

SIGNIFICANCE AND IMPACT OF THE STUDY

These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.

Authors+Show Affiliations

Cátedra de Fitoquímica, Instituto de Estudios Vegetales Dr. A.R. Sampietro, Facultad de Bioquímica, Química y Farmacia Universidad Nacional de Tucumán Ayacucho 471 (4000) San Miguel de Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17578409

Citation

Soberón, J R., et al. "Antibacterial Activity of Plant Extracts From Northwestern Argentina." Journal of Applied Microbiology, vol. 102, no. 6, 2007, pp. 1450-61.
Soberón JR, Sgariglia MA, Sampietro DA, et al. Antibacterial activity of plant extracts from northwestern Argentina. J Appl Microbiol. 2007;102(6):1450-61.
Soberón, J. R., Sgariglia, M. A., Sampietro, D. A., Quiroga, E. N., & Vattuone, M. A. (2007). Antibacterial activity of plant extracts from northwestern Argentina. Journal of Applied Microbiology, 102(6), pp. 1450-61.
Soberón JR, et al. Antibacterial Activity of Plant Extracts From Northwestern Argentina. J Appl Microbiol. 2007;102(6):1450-61. PubMed PMID: 17578409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activity of plant extracts from northwestern Argentina. AU - Soberón,J R, AU - Sgariglia,M A, AU - Sampietro,D A, AU - Quiroga,E N, AU - Vattuone,M A, PY - 2007/6/21/pubmed PY - 2007/12/14/medline PY - 2007/6/21/entrez SP - 1450 EP - 61 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 102 IS - 6 N2 - AIMS: To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. METHODS AND RESULTS: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD(50)) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 microg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 microg ml(-1)) against Acinetobacterfreundii (303). These data were lower than their LD(50). Tripodanthus acutifolius tincture showed lower MIC (110 microg of EM per ml) and minimal bactericidal concentration (MBC) (220 microg of EM per ml) than cefotaxim (MIC and MBC of 320 microg ml(-1)) for Pseudomonasaeruginosa. This extract also showed a MIC/MBC of 110/220 microg of EM per ml, lower than oxacillin (MIC/MBC of 160/220 microg ml(-1)) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3',4',5,7-pentahydroxyflavone 3-beta-rhamnosilglucoside), iso-quercitrin (3,3',4',5,7-pentahydroxyflavone 3-beta-glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. CONCLUSIONS: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. SIGNIFICANCE AND IMPACT OF THE STUDY: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/17578409/Antibacterial_activity_of_plant_extracts_from_northwestern_Argentina_ L2 - https://doi.org/10.1111/j.1365-2672.2006.03229.x DB - PRIME DP - Unbound Medicine ER -