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Performance characteristics of a polyclonal enzyme immunoassay for the quantitation of Histoplasma antigen in human urine samples.
Am J Clin Pathol. 2007 Jul; 128(1):18-22.AJ

Abstract

An antigen detection assay was prepared with rabbit anti- Histoplasma antibodies to detect and quantitate Histoplasma capsulatum antigen in urine samples. By using a 4-parameter curve fit, the assay calibration ranges from 2 to 1,000 enzyme immunoassay (EIA) units. We compared results for 99 urine samples with those of a reference laboratory, half of which tested positive or equivocal by that reference laboratory. Performance characteristics were further defined by studying the assay linearity, precision, percentage of positive agreement, and percentage of negative agreement. An acceptable correlation with the reference laboratory (R2=0.7174) was obtained with results ranging from less than 2 (negative samples) to 132 EIA units by the new method. Compared with the reference laboratory, the percentage of agreement for positive samples, excluding equivocal samples, was 92% and for negative samples was 98%. Crossreactivity occurred with culture filtrates of Paracoccidioides brasiliensis, Coccidioides immitis, and Blastomyces dermatiditis. No cross-reactivity was observed with Candida albicans or Aspergillus fumigatus culture filtrates. The current EIA for the detection and quantitation of H capsulatum antigen in urine specimens is a valid assay that agrees well with results from an established reference laboratory.

Authors+Show Affiliations

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17580268

Citation

Cloud, Joann L., et al. "Performance Characteristics of a Polyclonal Enzyme Immunoassay for the Quantitation of Histoplasma Antigen in Human Urine Samples." American Journal of Clinical Pathology, vol. 128, no. 1, 2007, pp. 18-22.
Cloud JL, Bauman SK, Neary BP, et al. Performance characteristics of a polyclonal enzyme immunoassay for the quantitation of Histoplasma antigen in human urine samples. Am J Clin Pathol. 2007;128(1):18-22.
Cloud, J. L., Bauman, S. K., Neary, B. P., Ludwig, K. G., & Ashwood, E. R. (2007). Performance characteristics of a polyclonal enzyme immunoassay for the quantitation of Histoplasma antigen in human urine samples. American Journal of Clinical Pathology, 128(1), 18-22.
Cloud JL, et al. Performance Characteristics of a Polyclonal Enzyme Immunoassay for the Quantitation of Histoplasma Antigen in Human Urine Samples. Am J Clin Pathol. 2007;128(1):18-22. PubMed PMID: 17580268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance characteristics of a polyclonal enzyme immunoassay for the quantitation of Histoplasma antigen in human urine samples. AU - Cloud,Joann L, AU - Bauman,Sean K, AU - Neary,Brandon P, AU - Ludwig,Katie G, AU - Ashwood,Edward R, PY - 2007/6/21/pubmed PY - 2007/7/27/medline PY - 2007/6/21/entrez SP - 18 EP - 22 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 128 IS - 1 N2 - An antigen detection assay was prepared with rabbit anti- Histoplasma antibodies to detect and quantitate Histoplasma capsulatum antigen in urine samples. By using a 4-parameter curve fit, the assay calibration ranges from 2 to 1,000 enzyme immunoassay (EIA) units. We compared results for 99 urine samples with those of a reference laboratory, half of which tested positive or equivocal by that reference laboratory. Performance characteristics were further defined by studying the assay linearity, precision, percentage of positive agreement, and percentage of negative agreement. An acceptable correlation with the reference laboratory (R2=0.7174) was obtained with results ranging from less than 2 (negative samples) to 132 EIA units by the new method. Compared with the reference laboratory, the percentage of agreement for positive samples, excluding equivocal samples, was 92% and for negative samples was 98%. Crossreactivity occurred with culture filtrates of Paracoccidioides brasiliensis, Coccidioides immitis, and Blastomyces dermatiditis. No cross-reactivity was observed with Candida albicans or Aspergillus fumigatus culture filtrates. The current EIA for the detection and quantitation of H capsulatum antigen in urine specimens is a valid assay that agrees well with results from an established reference laboratory. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/17580268/Performance_characteristics_of_a_polyclonal_enzyme_immunoassay_for_the_quantitation_of_Histoplasma_antigen_in_human_urine_samples_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/Q7879TYDW5D93QK7 DB - PRIME DP - Unbound Medicine ER -