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Can nitric oxide-generating compounds improve the oxidative stress response in experimentally diabetic rats?
Clin Exp Pharmacol Physiol. 2007 Jul; 34(7):586-93.CE

Abstract

1. The present study was designed to evaluate the protective effects of the nitric oxide (NO)-generating compounds L-arginine (L-Arg) and sodium nitroprusside (SNP) on oxidative stress markers in streptozotocin (STZ)-diabetic rats. 2. Diabetes was induced after a single intraperitoneal injection of STZ (60 mg/kg). Rats were divided into non-diabetic (control), diabetic and treated diabetic groups. The treated diabetic groups were supplemented with L-Arg (300 mg/kg), SNP (3 mg/kg per day) or glibenclamide (0.6 mg/kg per day) orally for 4 weeks. 3. At the end of the experiment, fasted rats were killed by cervical decapitation. Blood was collected for estimation of glucose, haemoglobin, glycosylated haemoglobin (HbA(1c)), total cholesterol, high-density lipoprotein-cholesterol and triglycerides. Thiobarbituric acid-reactive substances (TBARS; an index of lipid peroxidation), superoxide dismutase, glutathione peroxidase, catalase, nitrate/nitrite (NO(x)) and reduced glutathione (GSH) were estimated in liver and kidney homogenates. 4. A significant increase was observed in plasma glucose levels and HbA(1c), with a concomitant decrease in haemoglobin levels, in diabetic rats. These alterations reverted back to near normal after treatment with the NO-generating compounds. A loss of bodyweight, polydipsia, polyphagia and elevated levels of serum cholesterol and triglycerides were observed in diabetic rats. Hyperglycaemia was accompanied by a significant increase in tissue TBARS and a decrease in NO(x), GSH and anti-oxidant enzymes, whereas, supplementation with L-Arg and SNP significantly reduced TBARS levels and increased GSH and anti-oxidant enzyme activities. Linear regression analysis indicated that blood glucose and TBARS had a significant positive correlation with HbA(1c), whereas a negative correlation was observed between GSH and NO(x). 5. It is concluded that NO-generating compounds improve most of the biochemical abnormalities and anti-oxidant levels in diabetic rats. The beneficial effects of NO-generating compounds can be attributed to the generation of NO and/or enhanced anti-oxidant enzyme activities.

Authors+Show Affiliations

Biochemistry Department and Tumor Marker Oncology Research Unit, Faculty of Pharmacy Boy, Al-Azhar University, Cairo, Egypt. amohamadin@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17581213

Citation

Mohamadin, Ahmed M A., et al. "Can Nitric Oxide-generating Compounds Improve the Oxidative Stress Response in Experimentally Diabetic Rats?" Clinical and Experimental Pharmacology & Physiology, vol. 34, no. 7, 2007, pp. 586-93.
Mohamadin AM, Hammad LN, El-Bab MF, et al. Can nitric oxide-generating compounds improve the oxidative stress response in experimentally diabetic rats? Clin Exp Pharmacol Physiol. 2007;34(7):586-93.
Mohamadin, A. M., Hammad, L. N., El-Bab, M. F., & Gawad, H. S. (2007). Can nitric oxide-generating compounds improve the oxidative stress response in experimentally diabetic rats? Clinical and Experimental Pharmacology & Physiology, 34(7), 586-93.
Mohamadin AM, et al. Can Nitric Oxide-generating Compounds Improve the Oxidative Stress Response in Experimentally Diabetic Rats. Clin Exp Pharmacol Physiol. 2007;34(7):586-93. PubMed PMID: 17581213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Can nitric oxide-generating compounds improve the oxidative stress response in experimentally diabetic rats? AU - Mohamadin,Ahmed M A, AU - Hammad,Lamiaa N A, AU - El-Bab,Mohamed Fath, AU - Gawad,Hala S Abdel, PY - 2007/6/22/pubmed PY - 2007/8/2/medline PY - 2007/6/22/entrez SP - 586 EP - 93 JF - Clinical and experimental pharmacology & physiology JO - Clin. Exp. Pharmacol. Physiol. VL - 34 IS - 7 N2 - 1. The present study was designed to evaluate the protective effects of the nitric oxide (NO)-generating compounds L-arginine (L-Arg) and sodium nitroprusside (SNP) on oxidative stress markers in streptozotocin (STZ)-diabetic rats. 2. Diabetes was induced after a single intraperitoneal injection of STZ (60 mg/kg). Rats were divided into non-diabetic (control), diabetic and treated diabetic groups. The treated diabetic groups were supplemented with L-Arg (300 mg/kg), SNP (3 mg/kg per day) or glibenclamide (0.6 mg/kg per day) orally for 4 weeks. 3. At the end of the experiment, fasted rats were killed by cervical decapitation. Blood was collected for estimation of glucose, haemoglobin, glycosylated haemoglobin (HbA(1c)), total cholesterol, high-density lipoprotein-cholesterol and triglycerides. Thiobarbituric acid-reactive substances (TBARS; an index of lipid peroxidation), superoxide dismutase, glutathione peroxidase, catalase, nitrate/nitrite (NO(x)) and reduced glutathione (GSH) were estimated in liver and kidney homogenates. 4. A significant increase was observed in plasma glucose levels and HbA(1c), with a concomitant decrease in haemoglobin levels, in diabetic rats. These alterations reverted back to near normal after treatment with the NO-generating compounds. A loss of bodyweight, polydipsia, polyphagia and elevated levels of serum cholesterol and triglycerides were observed in diabetic rats. Hyperglycaemia was accompanied by a significant increase in tissue TBARS and a decrease in NO(x), GSH and anti-oxidant enzymes, whereas, supplementation with L-Arg and SNP significantly reduced TBARS levels and increased GSH and anti-oxidant enzyme activities. Linear regression analysis indicated that blood glucose and TBARS had a significant positive correlation with HbA(1c), whereas a negative correlation was observed between GSH and NO(x). 5. It is concluded that NO-generating compounds improve most of the biochemical abnormalities and anti-oxidant levels in diabetic rats. The beneficial effects of NO-generating compounds can be attributed to the generation of NO and/or enhanced anti-oxidant enzyme activities. SN - 0305-1870 UR - https://www.unboundmedicine.com/medline/citation/17581213/Can_nitric_oxide_generating_compounds_improve_the_oxidative_stress_response_in_experimentally_diabetic_rats L2 - https://doi.org/10.1111/j.1440-1681.2007.04622.x DB - PRIME DP - Unbound Medicine ER -