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The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland.
J Clin Microbiol 2007; 45(8):2554-63JC

Abstract

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) carrying pvl is an emerging problem worldwide. CA-MRSA tends to harbor staphylococcal cassette chromosome mec type IV (SCCmec IV), to be non-multiantibiotic resistant, and to have different genotypes from the local hospital-acquired MRSA (HA-MRSA). However, in Ireland, 80% of HA-MRSA isolates have the non-multiantibiotic-resistant genotype ST22-MRSA-IV. This study investigated MRSA isolates from Ireland (CA-MRSA, health care-associated MRSA, and HA-MRSA) for the carriage of pvl and determined the genotypic characteristics of all pvl-positive isolates identified. All 1,389 MRSA isolates were investigated by antibiogram-resistogram typing and SmaI DNA macrorestriction analysis. pvl-positive isolates were further characterized by multilocus sequence typing and SCCmec, agr, and toxin gene typing. Twenty-five (1.8%) MRSA isolates belonging to six genotypes (ST30, ST8, ST22, ST80, ST5, and ST154) harbored pvl. Nineteen of these (76%) were CA-MRSA isolates, but a prospective study of MRSA isolates from 401 patients showed that only 6.7% (2/30) of patients with CA-MRSA yielded pvl-positive isolates. Thus, pvl cannot be used as a sole marker for CA-MRSA. Fifty-two percent of pvl-positive MRSA isolates were recovered from patients with skin and soft tissue infections; thirty-six percent were from patients of non-Irish ethnic origin, reflecting the increasing heterogeneity of the Irish population due to immigration. All 25 pvl-positive isolates carried SCCmec IV; 14 (56%) harbored SCCmec IV.1 or IV.3, and the remaining 11 isolates could not be subtyped. This study demonstrates that pvl is not a reliable marker for CA-MRSA in Ireland and reveals the emergence and importation of diverse genotypes of pvl-positive MRSA in Ireland.

Authors+Show Affiliations

National MRSA Reference Laboratory, St. James's Hospital, James's St., Dublin 8, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17581935

Citation

Rossney, Angela S., et al. "The Emergence and Importation of Diverse Genotypes of Methicillin-resistant Staphylococcus Aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene (pvl) Reveal That Pvl Is a Poor Marker for Community-acquired MRSA Strains in Ireland." Journal of Clinical Microbiology, vol. 45, no. 8, 2007, pp. 2554-63.
Rossney AS, Shore AC, Morgan PM, et al. The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland. J Clin Microbiol. 2007;45(8):2554-63.
Rossney, A. S., Shore, A. C., Morgan, P. M., Fitzgibbon, M. M., O'Connell, B., & Coleman, D. C. (2007). The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland. Journal of Clinical Microbiology, 45(8), pp. 2554-63.
Rossney AS, et al. The Emergence and Importation of Diverse Genotypes of Methicillin-resistant Staphylococcus Aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene (pvl) Reveal That Pvl Is a Poor Marker for Community-acquired MRSA Strains in Ireland. J Clin Microbiol. 2007;45(8):2554-63. PubMed PMID: 17581935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland. AU - Rossney,Angela S, AU - Shore,Anna C, AU - Morgan,Pamela M, AU - Fitzgibbon,Margaret M, AU - O'Connell,Brian, AU - Coleman,David C, Y1 - 2007/06/20/ PY - 2007/6/22/pubmed PY - 2007/11/7/medline PY - 2007/6/22/entrez SP - 2554 EP - 63 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 45 IS - 8 N2 - Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) carrying pvl is an emerging problem worldwide. CA-MRSA tends to harbor staphylococcal cassette chromosome mec type IV (SCCmec IV), to be non-multiantibiotic resistant, and to have different genotypes from the local hospital-acquired MRSA (HA-MRSA). However, in Ireland, 80% of HA-MRSA isolates have the non-multiantibiotic-resistant genotype ST22-MRSA-IV. This study investigated MRSA isolates from Ireland (CA-MRSA, health care-associated MRSA, and HA-MRSA) for the carriage of pvl and determined the genotypic characteristics of all pvl-positive isolates identified. All 1,389 MRSA isolates were investigated by antibiogram-resistogram typing and SmaI DNA macrorestriction analysis. pvl-positive isolates were further characterized by multilocus sequence typing and SCCmec, agr, and toxin gene typing. Twenty-five (1.8%) MRSA isolates belonging to six genotypes (ST30, ST8, ST22, ST80, ST5, and ST154) harbored pvl. Nineteen of these (76%) were CA-MRSA isolates, but a prospective study of MRSA isolates from 401 patients showed that only 6.7% (2/30) of patients with CA-MRSA yielded pvl-positive isolates. Thus, pvl cannot be used as a sole marker for CA-MRSA. Fifty-two percent of pvl-positive MRSA isolates were recovered from patients with skin and soft tissue infections; thirty-six percent were from patients of non-Irish ethnic origin, reflecting the increasing heterogeneity of the Irish population due to immigration. All 25 pvl-positive isolates carried SCCmec IV; 14 (56%) harbored SCCmec IV.1 or IV.3, and the remaining 11 isolates could not be subtyped. This study demonstrates that pvl is not a reliable marker for CA-MRSA in Ireland and reveals the emergence and importation of diverse genotypes of pvl-positive MRSA in Ireland. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/17581935/The_emergence_and_importation_of_diverse_genotypes_of_methicillin_resistant_Staphylococcus_aureus__MRSA__harboring_the_Panton_Valentine_leukocidin_gene__pvl__reveal_that_pvl_is_a_poor_marker_for_community_acquired_MRSA_strains_in_Ireland_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=17581935 DB - PRIME DP - Unbound Medicine ER -