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A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles.
Biosens Bioelectron. 2007 Oct 31; 23(3):341-7.BB

Abstract

An ultrasensitive and highly specific electrochemical aptasensor for detection of thrombin based on gold nanoparticles and thiocyanuric acid is presented. For this proposed aptasensor, aptamerI was immobilized on the magnetic nanoparticles, aptamerII was labeled with gold nanoparticles. The magnetic nanoparticle was used for separation and collection, and gold nanoparticle offered excellent electrochemical signal transduction. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle/thrombin/gold nanoparticle was fabricated, and the signal amplification was further implemented by forming network-like thiocyanuric acid/gold nanoparticles. A significant sensitivity enhancement had been obtained, and the detection limit was down to 7.82 aM. The presence of other proteins such as BSA and lysozyme did not affect the detection of thrombin, which indicates a high specificity of thrombin detection could be achieved. This electrochemical aptasensor is expected to have wide applications in protein monitoring and disease diagnosis.

Authors+Show Affiliations

Department of Chemistry, East China Normal University, Shanghai 200062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17583489

Citation

Zheng, Jing, et al. "A New Amplification Strategy for Ultrasensitive Electrochemical Aptasensor With Network-like Thiocyanuric Acid/gold Nanoparticles." Biosensors & Bioelectronics, vol. 23, no. 3, 2007, pp. 341-7.
Zheng J, Feng W, Lin L, et al. A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. Biosens Bioelectron. 2007;23(3):341-7.
Zheng, J., Feng, W., Lin, L., Zhang, F., Cheng, G., He, P., & Fang, Y. (2007). A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. Biosensors & Bioelectronics, 23(3), 341-7.
Zheng J, et al. A New Amplification Strategy for Ultrasensitive Electrochemical Aptasensor With Network-like Thiocyanuric Acid/gold Nanoparticles. Biosens Bioelectron. 2007 Oct 31;23(3):341-7. PubMed PMID: 17583489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. AU - Zheng,Jing, AU - Feng,Wanjuan, AU - Lin,Li, AU - Zhang,Fan, AU - Cheng,Guifang, AU - He,Pingang, AU - Fang,Yuzhi, Y1 - 2007/05/03/ PY - 2007/01/11/received PY - 2007/04/20/revised PY - 2007/04/24/accepted PY - 2007/6/23/pubmed PY - 2008/1/18/medline PY - 2007/6/23/entrez SP - 341 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 3 N2 - An ultrasensitive and highly specific electrochemical aptasensor for detection of thrombin based on gold nanoparticles and thiocyanuric acid is presented. For this proposed aptasensor, aptamerI was immobilized on the magnetic nanoparticles, aptamerII was labeled with gold nanoparticles. The magnetic nanoparticle was used for separation and collection, and gold nanoparticle offered excellent electrochemical signal transduction. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle/thrombin/gold nanoparticle was fabricated, and the signal amplification was further implemented by forming network-like thiocyanuric acid/gold nanoparticles. A significant sensitivity enhancement had been obtained, and the detection limit was down to 7.82 aM. The presence of other proteins such as BSA and lysozyme did not affect the detection of thrombin, which indicates a high specificity of thrombin detection could be achieved. This electrochemical aptasensor is expected to have wide applications in protein monitoring and disease diagnosis. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17583489/A_new_amplification_strategy_for_ultrasensitive_electrochemical_aptasensor_with_network_like_thiocyanuric_acid/gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(07)00245-X DB - PRIME DP - Unbound Medicine ER -