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Nitrergic and purinergic interplay in inhibitory transmission in rat gastric fundus.
Auton Autacoid Pharmacol. 2007 Jul; 27(3):151-7.AA

Abstract

1 This study was undertaken to analyse the involvement of ATP in non-adrenergic non- cholinergic (NANC) relaxation and possible interplay between nitrergic and purinergic systems in rat gastric fundus. 2 Experiments were performed in vitro on strips of longitudinal muscle from rat gastric fundus, recording the mechanical activity as changes in isometric force. In addition, NO release induced by different experimental conditions was assayed. 3 Under NANC conditions in serotonin-precontracted strips, electrical field stimulation (EFS) elicited a tetrodotoxin (TTX)-sensitive relaxation accompanied by nitric oxide (NO) release. This effect was antagonized by pretreatment with the NO synthase antagonist Nomega-nitro-L-arginine (L-NA) or by desensitization of purinergic receptors. Purinergic desensitization was also able to further antagonize the residual EFS-induced relaxation remaining after L-NA treatment. Exogenously applied NO [delivered as sodium nitroprusside (SNP)] or ATP (and related purines) induced concentration-dependent, TTX-insensitive relaxant responses. ATP also induced the release of NO. A reduction in the responses to ATP was observed in the presence of L-NA. In contrast, SNP-induced relaxation remained unchanged after desensitization of purinergic receptors. Finally, apamin, a blocker of the small conductance Ca2+ -dependent K+ channels, reduced the amplitude of the muscular relaxation evoked by either EFS, ATP or SNP. 4 In conclusion, this study provides evidence that in rat gastric fundus, ATP is one of the inhibitory transmitters released from NANC intramural neurones acting directly on the muscle, through receptors coupled to apamin-sensitive Ca2+ -dependent K+ channels and, indirectly, through the stimulation of NO production.

Authors+Show Affiliations

Dipartimento di Biologia cellulare e dello Sviluppo, Laboratorio di Fisiologia Generale, Università degli Studi di Palermo, Viale delle Scienze, Parco D'Orleans II, Palermo, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17584445

Citation

Vetri, T, et al. "Nitrergic and Purinergic Interplay in Inhibitory Transmission in Rat Gastric Fundus." Autonomic & Autacoid Pharmacology, vol. 27, no. 3, 2007, pp. 151-7.
Vetri T, Bonvissuto F, Marino A, et al. Nitrergic and purinergic interplay in inhibitory transmission in rat gastric fundus. Auton Autacoid Pharmacol. 2007;27(3):151-7.
Vetri, T., Bonvissuto, F., Marino, A., & Postorino, A. (2007). Nitrergic and purinergic interplay in inhibitory transmission in rat gastric fundus. Autonomic & Autacoid Pharmacology, 27(3), 151-7.
Vetri T, et al. Nitrergic and Purinergic Interplay in Inhibitory Transmission in Rat Gastric Fundus. Auton Autacoid Pharmacol. 2007;27(3):151-7. PubMed PMID: 17584445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitrergic and purinergic interplay in inhibitory transmission in rat gastric fundus. AU - Vetri,T, AU - Bonvissuto,F, AU - Marino,A, AU - Postorino,A, PY - 2007/6/23/pubmed PY - 2007/9/5/medline PY - 2007/6/23/entrez SP - 151 EP - 7 JF - Autonomic & autacoid pharmacology JO - Auton Autacoid Pharmacol VL - 27 IS - 3 N2 - 1 This study was undertaken to analyse the involvement of ATP in non-adrenergic non- cholinergic (NANC) relaxation and possible interplay between nitrergic and purinergic systems in rat gastric fundus. 2 Experiments were performed in vitro on strips of longitudinal muscle from rat gastric fundus, recording the mechanical activity as changes in isometric force. In addition, NO release induced by different experimental conditions was assayed. 3 Under NANC conditions in serotonin-precontracted strips, electrical field stimulation (EFS) elicited a tetrodotoxin (TTX)-sensitive relaxation accompanied by nitric oxide (NO) release. This effect was antagonized by pretreatment with the NO synthase antagonist Nomega-nitro-L-arginine (L-NA) or by desensitization of purinergic receptors. Purinergic desensitization was also able to further antagonize the residual EFS-induced relaxation remaining after L-NA treatment. Exogenously applied NO [delivered as sodium nitroprusside (SNP)] or ATP (and related purines) induced concentration-dependent, TTX-insensitive relaxant responses. ATP also induced the release of NO. A reduction in the responses to ATP was observed in the presence of L-NA. In contrast, SNP-induced relaxation remained unchanged after desensitization of purinergic receptors. Finally, apamin, a blocker of the small conductance Ca2+ -dependent K+ channels, reduced the amplitude of the muscular relaxation evoked by either EFS, ATP or SNP. 4 In conclusion, this study provides evidence that in rat gastric fundus, ATP is one of the inhibitory transmitters released from NANC intramural neurones acting directly on the muscle, through receptors coupled to apamin-sensitive Ca2+ -dependent K+ channels and, indirectly, through the stimulation of NO production. SN - 1474-8665 UR - https://www.unboundmedicine.com/medline/citation/17584445/Nitrergic_and_purinergic_interplay_in_inhibitory_transmission_in_rat_gastric_fundus_ L2 - https://doi.org/10.1111/j.1474-8673.2007.00406.x DB - PRIME DP - Unbound Medicine ER -