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Temporomandibular joint inflammation decreases the voltage-gated K+ channel subtype 1.4-immunoreactivity of trigeminal ganglion neurons in rats.
Eur J Pain. 2008 Feb; 12(2):189-95.EJ

Abstract

Voltage-gated K+ (Kv) channels are one of the important physiological regulators of the membrane potentials in excitable cells, including sensory ganglion neurons. The aim of the present study was to investigate whether temporomandibular joint (TMJ) inflammation alters expression of Kv channel subtype 1.4 (Kv1.4) of trigeminal ganglion (TRG) neurons innervating TMJ relating allodynia (pain caused by normally innoxious stimulation), by using both behavioral and immunohistochemical techniques. TMJ inflammation was induced by injection of Complete Freund's Adjuvant (CFA) into the rat TMJ. The threshold for escape from mechanical stimulation applied to the orofacial area in TMJ inflamed rats was significantly lower than that in naïve rats. TMJ afferents were identified by fluorogold (FG) labeling. The mean numbers of Kv1.4-/neurofilament (NF) 200(myelinated fiber marker) positive- and negative-immunoreactivities FG-labeled small-/medium-diameter TRG neurons in inflamed rats were significantly decreased when compared with those in the naïve rats. These findings suggest that TMJ inflammation reduces the expression of Kv1.4 subunits in the small-/medium sized (Adelta-/C-) TRG neurons and this may contribute to trigeminal inflammatory allodynia in TMJ disorder. These results lead us to suggest that Kv channel openers may be a potential therapeutic agents for prevention of mechanical allodynia.

Authors+Show Affiliations

Department of Physiology, Nippon Dental University, 1-9-20, Fujimi-cho, Chiyoda-ku, Tokyo 102-8159, Japan. m-takeda@tokyo.ndu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17584507

Citation

Takeda, Mamoru, et al. "Temporomandibular Joint Inflammation Decreases the Voltage-gated K+ Channel Subtype 1.4-immunoreactivity of Trigeminal Ganglion Neurons in Rats." European Journal of Pain (London, England), vol. 12, no. 2, 2008, pp. 189-95.
Takeda M, Tanimoto T, Nasu M, et al. Temporomandibular joint inflammation decreases the voltage-gated K+ channel subtype 1.4-immunoreactivity of trigeminal ganglion neurons in rats. Eur J Pain. 2008;12(2):189-95.
Takeda, M., Tanimoto, T., Nasu, M., & Matsumoto, S. (2008). Temporomandibular joint inflammation decreases the voltage-gated K+ channel subtype 1.4-immunoreactivity of trigeminal ganglion neurons in rats. European Journal of Pain (London, England), 12(2), 189-95.
Takeda M, et al. Temporomandibular Joint Inflammation Decreases the Voltage-gated K+ Channel Subtype 1.4-immunoreactivity of Trigeminal Ganglion Neurons in Rats. Eur J Pain. 2008;12(2):189-95. PubMed PMID: 17584507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporomandibular joint inflammation decreases the voltage-gated K+ channel subtype 1.4-immunoreactivity of trigeminal ganglion neurons in rats. AU - Takeda,Mamoru, AU - Tanimoto,Takeshi, AU - Nasu,Masanori, AU - Matsumoto,Shigeji, Y1 - 2007/06/20/ PY - 2007/02/22/received PY - 2007/04/04/revised PY - 2007/04/22/accepted PY - 2007/6/23/pubmed PY - 2008/2/21/medline PY - 2007/6/23/entrez SP - 189 EP - 95 JF - European journal of pain (London, England) JO - Eur J Pain VL - 12 IS - 2 N2 - Voltage-gated K+ (Kv) channels are one of the important physiological regulators of the membrane potentials in excitable cells, including sensory ganglion neurons. The aim of the present study was to investigate whether temporomandibular joint (TMJ) inflammation alters expression of Kv channel subtype 1.4 (Kv1.4) of trigeminal ganglion (TRG) neurons innervating TMJ relating allodynia (pain caused by normally innoxious stimulation), by using both behavioral and immunohistochemical techniques. TMJ inflammation was induced by injection of Complete Freund's Adjuvant (CFA) into the rat TMJ. The threshold for escape from mechanical stimulation applied to the orofacial area in TMJ inflamed rats was significantly lower than that in naïve rats. TMJ afferents were identified by fluorogold (FG) labeling. The mean numbers of Kv1.4-/neurofilament (NF) 200(myelinated fiber marker) positive- and negative-immunoreactivities FG-labeled small-/medium-diameter TRG neurons in inflamed rats were significantly decreased when compared with those in the naïve rats. These findings suggest that TMJ inflammation reduces the expression of Kv1.4 subunits in the small-/medium sized (Adelta-/C-) TRG neurons and this may contribute to trigeminal inflammatory allodynia in TMJ disorder. These results lead us to suggest that Kv channel openers may be a potential therapeutic agents for prevention of mechanical allodynia. SN - 1532-2149 UR - https://www.unboundmedicine.com/medline/citation/17584507/Temporomandibular_joint_inflammation_decreases_the_voltage_gated_K+_channel_subtype_1_4_immunoreactivity_of_trigeminal_ganglion_neurons_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-3801(07)00562-9 DB - PRIME DP - Unbound Medicine ER -