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Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB.
Acta Pharmacol Sin. 2007 Jul; 28(7):1007-14.AP

Abstract

AIM

To investigate the mechanism of polypeptide from Chlamys farreri (PCF) protecting HaCaT cells from apoptosis induced by UVA plus UVB in vitro.

METHODS

An apoptotic model of UV irradiation-induced HaCaT cells was established. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, agarose gel electrophoresis, biochemical methods, and Western blotting were employed in the study.

RESULTS

PCF inhibited the UV irradiation-induced apoptosis of HaCaT cells. PCF strongly reduced the intracellular reactive oxygen species level, enhanced activities of superoxide dismutase and glutathione peroxidase and increased the total anti-oxidative capacity in HaCaT cells following UV irradiation. Furthermore, we found that PCF could inhibit the phosphorylation of c-Jun amino-terminal kinase and the activity of caspase-3 in a concentration-dependent manner.

CONCLUSION

PCF protected HaCaT cells from apoptosis induced by UVA plus UVB, mainly through decreasing the intracellular ROS level and increasing the activities of anti-oxidative enzymes to block the ROS-JNK-caspase-3-apoptosis signaling pathway.

Authors+Show Affiliations

Medical College, Qingdao University, Qingdao 266021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17588337

Citation

Gao, Ming-qing, et al. "Molecular Mechanisms of Polypeptide From Chlamys Farreri Protecting HaCaT Cells From Apoptosis Induced By UVA Plus UVB." Acta Pharmacologica Sinica, vol. 28, no. 7, 2007, pp. 1007-14.
Gao MQ, Guo SB, Chen XH, et al. Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB. Acta Pharmacol Sin. 2007;28(7):1007-14.
Gao, M. Q., Guo, S. B., Chen, X. H., Du, W., & Wang, C. B. (2007). Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB. Acta Pharmacologica Sinica, 28(7), 1007-14.
Gao MQ, et al. Molecular Mechanisms of Polypeptide From Chlamys Farreri Protecting HaCaT Cells From Apoptosis Induced By UVA Plus UVB. Acta Pharmacol Sin. 2007;28(7):1007-14. PubMed PMID: 17588337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB. AU - Gao,Ming-qing, AU - Guo,Shen-bo, AU - Chen,Xue-hong, AU - Du,Wei, AU - Wang,Chun-bo, PY - 2007/6/26/pubmed PY - 2009/4/21/medline PY - 2007/6/26/entrez SP - 1007 EP - 14 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 28 IS - 7 N2 - AIM: To investigate the mechanism of polypeptide from Chlamys farreri (PCF) protecting HaCaT cells from apoptosis induced by UVA plus UVB in vitro. METHODS: An apoptotic model of UV irradiation-induced HaCaT cells was established. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, agarose gel electrophoresis, biochemical methods, and Western blotting were employed in the study. RESULTS: PCF inhibited the UV irradiation-induced apoptosis of HaCaT cells. PCF strongly reduced the intracellular reactive oxygen species level, enhanced activities of superoxide dismutase and glutathione peroxidase and increased the total anti-oxidative capacity in HaCaT cells following UV irradiation. Furthermore, we found that PCF could inhibit the phosphorylation of c-Jun amino-terminal kinase and the activity of caspase-3 in a concentration-dependent manner. CONCLUSION: PCF protected HaCaT cells from apoptosis induced by UVA plus UVB, mainly through decreasing the intracellular ROS level and increasing the activities of anti-oxidative enzymes to block the ROS-JNK-caspase-3-apoptosis signaling pathway. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/17588337/Molecular_mechanisms_of_polypeptide_from_Chlamys_farreri_protecting_HaCaT_cells_from_apoptosis_induced_by_UVA_plus_UVB_ L2 - https://doi.org/10.1111/j.1745-7254.2007.00594.x DB - PRIME DP - Unbound Medicine ER -