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N-acetylcysteine attenuates lung ischemia-reperfusion injury after lung transplantation.
Ann Thorac Surg. 2007 Jul; 84(1):240-6; discussion 246.AT

Abstract

BACKGROUND

Early acute graft dysfunction continues to be a problem after lung transplantation and results in significant postoperative morbidity and mortality. This study assessed the protective effect of N-acetylcysteine (NAC) on posttransplant lung ischemia-reperfusion injury.

METHODS

Rat single-lung transplantation was performed in two experimental groups (n = 5) after 18 hours of cold (4 degrees C) ischemia. Group I was the ischemic control (IC) group. In group II (NAC), donor and recipient animals were treated with an intraperitoneal injection of 150 mg/kg NAC 15 minutes before harvest, and recipient animals were treated again before reperfusion. After 2 hours of reperfusion, oxygenation was measured. Lung tissue was assessed for lipid peroxidation, neutrophil infiltration, and reduced glutathione level. Peak airway pressure was recorded throughout the reperfusion period.

RESULTS

Rats treated with NAC showed significantly better oxygenation (184.5 +/- 83.3 mm Hg versus 67.3 +/- 16.4 mm Hg, p = 0.016) and reduced lipid peroxidation (7.34 +/- 1.9 micromol/g versus 17.46 +/- 10.6 micromol/g, p = 0.016). Lung tissue reduced glutathione levels were 6.8 +/- 0.9 microM in the IC group and 20.6 +/- 2.4 microM in the NAC group (p = 0.004). Peak airway pressure at the end of the reperfusion period was 14.4 +/- 1.6 cm H2O in the NAC group, and 19.2 +/- 2.2 cm H2O in the IC group (p = 0.008). Myeloperoxidase activity and the ratio of wet-to-dry weight did not differ between the groups.

CONCLUSIONS

In this model, exogenously administered NAC effectively protected the lungs from reperfusion injury after prolonged ischemia.

Authors+Show Affiliations

Division of Thoracic Surgery, University of Zurich, Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17588422

Citation

Inci, Ilhan, et al. "N-acetylcysteine Attenuates Lung Ischemia-reperfusion Injury After Lung Transplantation." The Annals of Thoracic Surgery, vol. 84, no. 1, 2007, pp. 240-6; discussion 246.
Inci I, Zhai W, Arni S, et al. N-acetylcysteine attenuates lung ischemia-reperfusion injury after lung transplantation. Ann Thorac Surg. 2007;84(1):240-6; discussion 246.
Inci, I., Zhai, W., Arni, S., Hillinger, S., Vogt, P., & Weder, W. (2007). N-acetylcysteine attenuates lung ischemia-reperfusion injury after lung transplantation. The Annals of Thoracic Surgery, 84(1), 240-6; discussion 246.
Inci I, et al. N-acetylcysteine Attenuates Lung Ischemia-reperfusion Injury After Lung Transplantation. Ann Thorac Surg. 2007;84(1):240-6; discussion 246. PubMed PMID: 17588422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-acetylcysteine attenuates lung ischemia-reperfusion injury after lung transplantation. AU - Inci,Ilhan, AU - Zhai,Wei, AU - Arni,Stephan, AU - Hillinger,Sven, AU - Vogt,Peter, AU - Weder,Walter, PY - 2007/01/10/received PY - 2007/03/23/revised PY - 2007/03/26/accepted PY - 2007/6/26/pubmed PY - 2007/7/21/medline PY - 2007/6/26/entrez SP - 240-6; discussion 246 JF - The Annals of thoracic surgery JO - Ann Thorac Surg VL - 84 IS - 1 N2 - BACKGROUND: Early acute graft dysfunction continues to be a problem after lung transplantation and results in significant postoperative morbidity and mortality. This study assessed the protective effect of N-acetylcysteine (NAC) on posttransplant lung ischemia-reperfusion injury. METHODS: Rat single-lung transplantation was performed in two experimental groups (n = 5) after 18 hours of cold (4 degrees C) ischemia. Group I was the ischemic control (IC) group. In group II (NAC), donor and recipient animals were treated with an intraperitoneal injection of 150 mg/kg NAC 15 minutes before harvest, and recipient animals were treated again before reperfusion. After 2 hours of reperfusion, oxygenation was measured. Lung tissue was assessed for lipid peroxidation, neutrophil infiltration, and reduced glutathione level. Peak airway pressure was recorded throughout the reperfusion period. RESULTS: Rats treated with NAC showed significantly better oxygenation (184.5 +/- 83.3 mm Hg versus 67.3 +/- 16.4 mm Hg, p = 0.016) and reduced lipid peroxidation (7.34 +/- 1.9 micromol/g versus 17.46 +/- 10.6 micromol/g, p = 0.016). Lung tissue reduced glutathione levels were 6.8 +/- 0.9 microM in the IC group and 20.6 +/- 2.4 microM in the NAC group (p = 0.004). Peak airway pressure at the end of the reperfusion period was 14.4 +/- 1.6 cm H2O in the NAC group, and 19.2 +/- 2.2 cm H2O in the IC group (p = 0.008). Myeloperoxidase activity and the ratio of wet-to-dry weight did not differ between the groups. CONCLUSIONS: In this model, exogenously administered NAC effectively protected the lungs from reperfusion injury after prolonged ischemia. SN - 1552-6259 UR - https://www.unboundmedicine.com/medline/citation/17588422/N_acetylcysteine_attenuates_lung_ischemia_reperfusion_injury_after_lung_transplantation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-4975(07)00692-3 DB - PRIME DP - Unbound Medicine ER -