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Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies.
Analyst. 2007 Jul; 132(7):679-86.A

Abstract

Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold.

Authors+Show Affiliations

Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada H4P 2R2. ghinwa.naja@nrc.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17592587

Citation

Naja, Ghinwa, et al. "Raman-based Detection of Bacteria Using Silver Nanoparticles Conjugated With Antibodies." The Analyst, vol. 132, no. 7, 2007, pp. 679-86.
Naja G, Bouvrette P, Hrapovic S, et al. Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies. Analyst. 2007;132(7):679-86.
Naja, G., Bouvrette, P., Hrapovic, S., & Luong, J. H. (2007). Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies. The Analyst, 132(7), 679-86.
Naja G, et al. Raman-based Detection of Bacteria Using Silver Nanoparticles Conjugated With Antibodies. Analyst. 2007;132(7):679-86. PubMed PMID: 17592587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies. AU - Naja,Ghinwa, AU - Bouvrette,Pierre, AU - Hrapovic,Sabahudin, AU - Luong,John H T, Y1 - 2007/06/05/ PY - 2007/6/27/pubmed PY - 2007/11/9/medline PY - 2007/6/27/entrez SP - 679 EP - 86 JF - The Analyst JO - Analyst VL - 132 IS - 7 N2 - Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/17592587/Raman_based_detection_of_bacteria_using_silver_nanoparticles_conjugated_with_antibodies_ DB - PRIME DP - Unbound Medicine ER -