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Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases.

Abstract

BACKGROUND

Use of effective scolicidal agents during puncture, aspiration or injection of a scolicidal agent and reaspiration (PAIR) and surgery for hydatid cysts are essential to reduce the recurrence rate. In this in vitro study, we tried to determine the scolicidal property of a new agent, octenidine dihydrochloride, and of various agents in different concentrations and exposure times.

MATERIAL AND METHODS

Echinococcus granulosus protoscoleces were obtained from six patients with liver (n=3) and lung (n=3) hydatid cysts. Various concentrations of octenidine dihydrochloride (0.1%, 0.01% and 0.001% diluted form), povidone iodine (10%, 1% and 0.1% diluted) and 20% saline were used in this study. Viability of protoscoleces was determined with dye-uptake (0.1% eosin) and flame cell activity.

RESULTS

Octenidine dihydrochloride 0.1% had strong scolicidal effect in 15 min and octenidine dihydrochloride 0.01% in 30 min. Sixty percent of protoscoleces lost viability at 5 min with octenidine dihydrochloride 0.1%. Viability ratio decreased to 20% at 10 min, and all of them died at 15 min. Povidone iodine 10% and 1% had strong scolicidal effects after 15- and 30 min of exposure, respectively. Saline 20% killed all the protoscoleces in 30-min exposure.

CONCLUSION

Because of the rapid and strong scolocidal effectiveness of octenidine dihydrochloride on protoscoleces, it may be used as a scolocidal agent during both perioperative and in the PAIR method.

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  • Authors+Show Affiliations

    ,

    Department of Microbiology, Afyon Kocatepe University School of Medicine, Afyon, Turkey. ihciftci@hotmail.com

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    Source

    MeSH

    Animals
    Antiparasitic Agents
    Echinococcosis, Hepatic
    Echinococcosis, Pulmonary
    Echinococcus granulosus
    Humans
    In Vitro Techniques
    Injections
    Perioperative Care
    Povidone-Iodine
    Pyridines
    Sodium Chloride

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    17595938

    Citation

    Ciftci, Ihsan Hakki, et al. "Effect of Octenidine Dihydrochloride On Viability of Protoscoleces in Hepatic and Pulmonary Hydatid Diseases." Journal of the National Medical Association, vol. 99, no. 6, 2007, pp. 674-7.
    Ciftci IH, Esme H, Sahin DA, et al. Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases. J Natl Med Assoc. 2007;99(6):674-7.
    Ciftci, I. H., Esme, H., Sahin, D. A., Solak, O., Sezer, M., & Dilek, O. N. (2007). Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases. Journal of the National Medical Association, 99(6), pp. 674-7.
    Ciftci IH, et al. Effect of Octenidine Dihydrochloride On Viability of Protoscoleces in Hepatic and Pulmonary Hydatid Diseases. J Natl Med Assoc. 2007;99(6):674-7. PubMed PMID: 17595938.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases. AU - Ciftci,Ihsan Hakki, AU - Esme,Hidir, AU - Sahin,Dursun Ali, AU - Solak,Okan, AU - Sezer,Murat, AU - Dilek,Osman Nuri, PY - 2007/6/29/pubmed PY - 2007/9/29/medline PY - 2007/6/29/entrez SP - 674 EP - 7 JF - Journal of the National Medical Association JO - J Natl Med Assoc VL - 99 IS - 6 N2 - BACKGROUND: Use of effective scolicidal agents during puncture, aspiration or injection of a scolicidal agent and reaspiration (PAIR) and surgery for hydatid cysts are essential to reduce the recurrence rate. In this in vitro study, we tried to determine the scolicidal property of a new agent, octenidine dihydrochloride, and of various agents in different concentrations and exposure times. MATERIAL AND METHODS: Echinococcus granulosus protoscoleces were obtained from six patients with liver (n=3) and lung (n=3) hydatid cysts. Various concentrations of octenidine dihydrochloride (0.1%, 0.01% and 0.001% diluted form), povidone iodine (10%, 1% and 0.1% diluted) and 20% saline were used in this study. Viability of protoscoleces was determined with dye-uptake (0.1% eosin) and flame cell activity. RESULTS: Octenidine dihydrochloride 0.1% had strong scolicidal effect in 15 min and octenidine dihydrochloride 0.01% in 30 min. Sixty percent of protoscoleces lost viability at 5 min with octenidine dihydrochloride 0.1%. Viability ratio decreased to 20% at 10 min, and all of them died at 15 min. Povidone iodine 10% and 1% had strong scolicidal effects after 15- and 30 min of exposure, respectively. Saline 20% killed all the protoscoleces in 30-min exposure. CONCLUSION: Because of the rapid and strong scolocidal effectiveness of octenidine dihydrochloride on protoscoleces, it may be used as a scolocidal agent during both perioperative and in the PAIR method. SN - 0027-9684 UR - https://www.unboundmedicine.com/medline/citation/17595938/Effect_of_octenidine_dihydrochloride_on_viability_of_protoscoleces_in_hepatic_and_pulmonary_hydatid_diseases_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/17595938/ DB - PRIME DP - Unbound Medicine ER -