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Detection of circulating galactomannan in serum samples for diagnosis of Penicillium marneffei infection and cryptococcosis among patients infected with human immunodeficiency virus.
J Clin Microbiol. 2007 Sep; 45(9):2858-62.JC

Abstract

Galactomannan (GM) is a heteropolysaccharide in the cell walls of most Aspergillus and Penicillium species. Cross-reactivity of Cryptococcus neoformans galactoxylomannan in an Aspergillus GM test has also been reported. In this study, we used a Platelia Aspergillus enzyme immunoassay kit (Bio-Rad) to test serum samples obtained from 48 human immunodeficiency virus (HIV)-infected patients (15 with penicilliosis [7 with fungemia alone, 4 with cavitary lung lesions alone, 3 with both fungemia and cavitary lung lesions, and 1 with disseminated disease], 22 with cryptococcosis [11 with fungemia alone, 5 with cavitary lung lesions, 3 with both, and 3 with meningitis alone], and 11 without any invasive fungal infection [control]) for GM levels. None of the patients had aspergillosis or concurrent use of piperacillin-tazobactam or amoxicillin-clavulanate. The median time between diagnosis of fungal infection and collection of serum samples was 0 days for penicilliosis and 1.5 days for cryptococcosis. Of patients with penicilliosis, cryptococcosis, and controls, 73.3%, 13.6%, and 9%, respectively, had GM optical density (OD) indices of >0.5 (P = 0.0001). GM OD indices were higher for penicilliosis (median OD index, 4.419; range, 0.158 to >20) than for cryptococcosis (median, 0.247; range, 0.112 to 3.849) cases (P < 0.001). Patients with fungemic penicilliosis had higher OD indices (median, 10.628; range, 0.401 to >20) than patients with nonfungemic penicilliosis (median, 0.378; range, 0.158 to 4.419) and patients with cryptococcemia (median, 0.231; range, 0.112 to 1.168) (P < 0.001). Of the 15 patients with cavitary lung lesions, those with penicilliosis had higher antigen levels (median OD index, 1.641; range, 0.247 to >20) than those with cryptococcosis (median, 0.227; range, 0.112 to 3.849) (P = 0.011). This study showed that the GM OD index was significantly elevated for HIV patients with penicilliosis. The use of the GM antigen assay may facilitate earlier diagnosis of Penicillium marneffei infection for HIV-infected patients in areas of endemicity.

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Authors+Show Affiliations

Huang YT
Department of Internal Medicine, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei 100, Taiwan.
Hung CC
No affiliation info available
Liao CH
No affiliation info available
Sun HY
No affiliation info available
Chang SC
No affiliation info available
Chen YC
No affiliation info available

MeSH

AdultAgedAged, 80 and overCryptococcosisCryptococcusFemaleFungemiaHIV InfectionsHumansLung Diseases, FungalMaleMannansMiddle AgedMycosesPenicilliumSerum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17596363

Citation

Huang, Yu-Tsung, et al. "Detection of Circulating Galactomannan in Serum Samples for Diagnosis of Penicillium Marneffei Infection and Cryptococcosis Among Patients Infected With Human Immunodeficiency Virus." Journal of Clinical Microbiology, vol. 45, no. 9, 2007, pp. 2858-62.
Huang YT, Hung CC, Liao CH, et al. Detection of circulating galactomannan in serum samples for diagnosis of Penicillium marneffei infection and cryptococcosis among patients infected with human immunodeficiency virus. J Clin Microbiol. 2007;45(9):2858-62.
Huang, Y. T., Hung, C. C., Liao, C. H., Sun, H. Y., Chang, S. C., & Chen, Y. C. (2007). Detection of circulating galactomannan in serum samples for diagnosis of Penicillium marneffei infection and cryptococcosis among patients infected with human immunodeficiency virus. Journal of Clinical Microbiology, 45(9), 2858-62.
Huang YT, et al. Detection of Circulating Galactomannan in Serum Samples for Diagnosis of Penicillium Marneffei Infection and Cryptococcosis Among Patients Infected With Human Immunodeficiency Virus. J Clin Microbiol. 2007;45(9):2858-62. PubMed PMID: 17596363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of circulating galactomannan in serum samples for diagnosis of Penicillium marneffei infection and cryptococcosis among patients infected with human immunodeficiency virus. AU - Huang,Yu-Tsung, AU - Hung,Chien-Ching, AU - Liao,Chun-Hsing, AU - Sun,Hsin-Yun, AU - Chang,Shan-Chwen, AU - Chen,Yee-Chun, Y1 - 2007/06/27/ PY - 2007/6/29/pubmed PY - 2007/11/7/medline PY - 2007/6/29/entrez SP - 2858 EP - 62 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 45 IS - 9 N2 - Galactomannan (GM) is a heteropolysaccharide in the cell walls of most Aspergillus and Penicillium species. Cross-reactivity of Cryptococcus neoformans galactoxylomannan in an Aspergillus GM test has also been reported. In this study, we used a Platelia Aspergillus enzyme immunoassay kit (Bio-Rad) to test serum samples obtained from 48 human immunodeficiency virus (HIV)-infected patients (15 with penicilliosis [7 with fungemia alone, 4 with cavitary lung lesions alone, 3 with both fungemia and cavitary lung lesions, and 1 with disseminated disease], 22 with cryptococcosis [11 with fungemia alone, 5 with cavitary lung lesions, 3 with both, and 3 with meningitis alone], and 11 without any invasive fungal infection [control]) for GM levels. None of the patients had aspergillosis or concurrent use of piperacillin-tazobactam or amoxicillin-clavulanate. The median time between diagnosis of fungal infection and collection of serum samples was 0 days for penicilliosis and 1.5 days for cryptococcosis. Of patients with penicilliosis, cryptococcosis, and controls, 73.3%, 13.6%, and 9%, respectively, had GM optical density (OD) indices of >0.5 (P = 0.0001). GM OD indices were higher for penicilliosis (median OD index, 4.419; range, 0.158 to >20) than for cryptococcosis (median, 0.247; range, 0.112 to 3.849) cases (P < 0.001). Patients with fungemic penicilliosis had higher OD indices (median, 10.628; range, 0.401 to >20) than patients with nonfungemic penicilliosis (median, 0.378; range, 0.158 to 4.419) and patients with cryptococcemia (median, 0.231; range, 0.112 to 1.168) (P < 0.001). Of the 15 patients with cavitary lung lesions, those with penicilliosis had higher antigen levels (median OD index, 1.641; range, 0.247 to >20) than those with cryptococcosis (median, 0.227; range, 0.112 to 3.849) (P = 0.011). This study showed that the GM OD index was significantly elevated for HIV patients with penicilliosis. The use of the GM antigen assay may facilitate earlier diagnosis of Penicillium marneffei infection for HIV-infected patients in areas of endemicity. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/17596363/Detection_of_circulating_galactomannan_in_serum_samples_for_diagnosis_of_Penicillium_marneffei_infection_and_cryptococcosis_among_patients_infected_with_human_immunodeficiency_virus_ DB - PRIME DP - Unbound Medicine ER -