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Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells.
J Neurosci. 2007 Jun 27; 27(26):7028-40.JN

Abstract

Neuronal progenitors in the mammalian brain derive from radial glia or specialized astrocytes. In developing neural retina, radial glia-like Müller cells are generated late in neurogenesis and are not considered to be neuronal progenitors, but they do proliferate after injury and can express neuronal markers, suggesting a latent neurogenic capacity. To examine the neurogenic capacity of retinal glial cells, we used lineage tracing in transgenic zebrafish with a glial-specific promoter (gfap, for glial fibrillary acid protein) driving green fluorescent protein in differentiated Müller glia. We found that all Müller glia in the zebrafish retina express low levels of the multipotent progenitor marker Pax6 (paired box gene 6), and they proliferate at a low frequency in the intact, uninjured retina. Müller glia-derived progenitors express Crx (cone rod homeobox) and are late retinal progenitors that generate the rod photoreceptor lineage in the postembryonic retina. These Müller glia-derived progenitors also remain competent to produce earlier neuronal lineages, in that they respond to loss of cone photoreceptors by specifically regenerating the missing neurons. We conclude that zebrafish Müller glia function as multipotent retinal stem cells that generate retinal neurons by homeostatic and regenerative developmental mechanisms.

Authors+Show Affiliations

Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48109-1048, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17596452

Citation

Bernardos, Rebecca L., et al. "Late-stage Neuronal Progenitors in the Retina Are Radial Müller Glia That Function as Retinal Stem Cells." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 27, no. 26, 2007, pp. 7028-40.
Bernardos RL, Barthel LK, Meyers JR, et al. Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells. J Neurosci. 2007;27(26):7028-40.
Bernardos, R. L., Barthel, L. K., Meyers, J. R., & Raymond, P. A. (2007). Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 27(26), 7028-40.
Bernardos RL, et al. Late-stage Neuronal Progenitors in the Retina Are Radial Müller Glia That Function as Retinal Stem Cells. J Neurosci. 2007 Jun 27;27(26):7028-40. PubMed PMID: 17596452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells. AU - Bernardos,Rebecca L, AU - Barthel,Linda K, AU - Meyers,Jason R, AU - Raymond,Pamela A, PY - 2007/6/29/pubmed PY - 2007/8/2/medline PY - 2007/6/29/entrez SP - 7028 EP - 40 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 27 IS - 26 N2 - Neuronal progenitors in the mammalian brain derive from radial glia or specialized astrocytes. In developing neural retina, radial glia-like Müller cells are generated late in neurogenesis and are not considered to be neuronal progenitors, but they do proliferate after injury and can express neuronal markers, suggesting a latent neurogenic capacity. To examine the neurogenic capacity of retinal glial cells, we used lineage tracing in transgenic zebrafish with a glial-specific promoter (gfap, for glial fibrillary acid protein) driving green fluorescent protein in differentiated Müller glia. We found that all Müller glia in the zebrafish retina express low levels of the multipotent progenitor marker Pax6 (paired box gene 6), and they proliferate at a low frequency in the intact, uninjured retina. Müller glia-derived progenitors express Crx (cone rod homeobox) and are late retinal progenitors that generate the rod photoreceptor lineage in the postembryonic retina. These Müller glia-derived progenitors also remain competent to produce earlier neuronal lineages, in that they respond to loss of cone photoreceptors by specifically regenerating the missing neurons. We conclude that zebrafish Müller glia function as multipotent retinal stem cells that generate retinal neurons by homeostatic and regenerative developmental mechanisms. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/17596452/Late_stage_neuronal_progenitors_in_the_retina_are_radial_Müller_glia_that_function_as_retinal_stem_cells_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=17596452 DB - PRIME DP - Unbound Medicine ER -