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Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair.
J Chromatogr A. 2007 Sep 07; 1163(1-2):43-8.JC

Abstract

We developed a rapid sample preparation method for the toxicological analysis of methamphetamine and amphetamine (the major metabolite of methamphetamine) in human hair by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), to facilitate fast screening and quantitation. Two milligrams of hair were mechanically micropulverized for 5 min in a 2-ml plastic tube together with 100 microl of an aqueous solvent containing 10% acetonitrile, 100 mM trifluoroacetic acid and the corresponding deuterium analogues as internal standards. The pulverizing highly disintegrated the hair components, simultaneously allowing the extraction of any drugs present in the hair. After filtering the suspension with a membrane-filter unit, the clear filtrate was directly analyzed by HPLC-MS/MS. No evaporation processes were required for sample preparation. Method optimization and validation study were carried out using real-case specimens and fortified samples in which the drugs had been artificially absorbed, respectively. Concentration ranges for quantitation were 0.040-125 and 0.040-25 ng/mg for methamphetamine and amphetamine, respectively. Real-case specimens were analyzed by the method presented here and by conventional ones to verify the applicability of our method to real-world analysis. Our method took less than 30 min for a set of chromatograms to be obtained from a washed hair sample.

Authors+Show Affiliations

National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. miyaguchi@nrips.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17597137

Citation

Miyaguchi, Hajime, et al. "Development of a Micropulverized Extraction Method for Rapid Toxicological Analysis of Methamphetamine in Hair." Journal of Chromatography. A, vol. 1163, no. 1-2, 2007, pp. 43-8.
Miyaguchi H, Kakuta M, Iwata YT, et al. Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair. J Chromatogr A. 2007;1163(1-2):43-8.
Miyaguchi, H., Kakuta, M., Iwata, Y. T., Matsuda, H., Tazawa, H., Kimura, H., & Inoue, H. (2007). Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair. Journal of Chromatography. A, 1163(1-2), 43-8.
Miyaguchi H, et al. Development of a Micropulverized Extraction Method for Rapid Toxicological Analysis of Methamphetamine in Hair. J Chromatogr A. 2007 Sep 7;1163(1-2):43-8. PubMed PMID: 17597137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair. AU - Miyaguchi,Hajime, AU - Kakuta,Masaya, AU - Iwata,Yuko T, AU - Matsuda,Hideaki, AU - Tazawa,Hidekatsu, AU - Kimura,Hiroko, AU - Inoue,Hiroyuki, Y1 - 2007/06/16/ PY - 2007/03/14/received PY - 2007/05/25/revised PY - 2007/06/12/accepted PY - 2007/6/29/pubmed PY - 2007/12/6/medline PY - 2007/6/29/entrez SP - 43 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1163 IS - 1-2 N2 - We developed a rapid sample preparation method for the toxicological analysis of methamphetamine and amphetamine (the major metabolite of methamphetamine) in human hair by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), to facilitate fast screening and quantitation. Two milligrams of hair were mechanically micropulverized for 5 min in a 2-ml plastic tube together with 100 microl of an aqueous solvent containing 10% acetonitrile, 100 mM trifluoroacetic acid and the corresponding deuterium analogues as internal standards. The pulverizing highly disintegrated the hair components, simultaneously allowing the extraction of any drugs present in the hair. After filtering the suspension with a membrane-filter unit, the clear filtrate was directly analyzed by HPLC-MS/MS. No evaporation processes were required for sample preparation. Method optimization and validation study were carried out using real-case specimens and fortified samples in which the drugs had been artificially absorbed, respectively. Concentration ranges for quantitation were 0.040-125 and 0.040-25 ng/mg for methamphetamine and amphetamine, respectively. Real-case specimens were analyzed by the method presented here and by conventional ones to verify the applicability of our method to real-world analysis. Our method took less than 30 min for a set of chromatograms to be obtained from a washed hair sample. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17597137/Development_of_a_micropulverized_extraction_method_for_rapid_toxicological_analysis_of_methamphetamine_in_hair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)01060-6 DB - PRIME DP - Unbound Medicine ER -