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Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia.
J Med Virol. 2007 Aug; 79(8):1128-37.JM

Abstract

Although no outbreaks of hepatitis E have been reported in Mongolia, a significant proportion of the general population had antibodies to hepatitis E virus (HEV). To investigate whether pigs are possible reservoirs of HEV in Mongolia, serum samples obtained from 243 2- or 3-month-old pigs on four swine farms surrounding Ulaanbaatar, the capital city of Mongolia, were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 223 pigs (91.8%) tested positive for anti-HEV, while 89 pigs (36.6%) had detectable HEV RNA. The 89 HEV isolates obtained from the viremic pigs were 78.7-100% identical to each other, and 80.9-85.9% similar to the prototype genotype 3 HEV isolate (US1) in the 412-nucleotide (nt) sequence within open reading frame 2. They were classified into two novel phylogenetic groups within genotype 3, differing by 16.4-21.3%. The swMN06-A1288 and swMN06-C1056 isolates, representing each of the two clusters within genotype 3, had a genomic length of nucleotides (nt) 7,222 nt and 7,223 nt, respectively, excluding the poly(A) tail, and shared only 81.6% over the entire genome. Upon comparison with the 25-reported genotype 3 HEV isolates over the entire genome, swMN06-A1288 had identities of merely up to 84.9%, while swMN06-C1056 of only up to 85.9%. Phylogenetic analysis confirmed the remote relatedness of the Mongolian swine isolates to the genotype 3 HEV isolates reported thus far. These results indicate that farm pigs in Mongolia are frequently infected with presumably indigenous HEV strains of genotype 3 and could be a source of HEV infections in humans in Mongolia.

Authors+Show Affiliations

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Tochigi-Ken, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17597486

Citation

Lorenzo, Felipe R., et al. "Analysis of the Full-length Genome of Hepatitis E Virus Isolates Obtained From Farm Pigs in Mongolia." Journal of Medical Virology, vol. 79, no. 8, 2007, pp. 1128-37.
Lorenzo FR, Tsatsralt-Od B, Ganbat S, et al. Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia. J Med Virol. 2007;79(8):1128-37.
Lorenzo, F. R., Tsatsralt-Od, B., Ganbat, S., Takahashi, M., & Okamoto, H. (2007). Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia. Journal of Medical Virology, 79(8), 1128-37.
Lorenzo FR, et al. Analysis of the Full-length Genome of Hepatitis E Virus Isolates Obtained From Farm Pigs in Mongolia. J Med Virol. 2007;79(8):1128-37. PubMed PMID: 17597486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the full-length genome of hepatitis E virus isolates obtained from farm pigs in Mongolia. AU - Lorenzo,Felipe R, AU - Tsatsralt-Od,Bira, AU - Ganbat,Sanjaa, AU - Takahashi,Masaharu, AU - Okamoto,Hiroaki, PY - 2007/6/29/pubmed PY - 2007/8/4/medline PY - 2007/6/29/entrez SP - 1128 EP - 37 JF - Journal of medical virology JO - J Med Virol VL - 79 IS - 8 N2 - Although no outbreaks of hepatitis E have been reported in Mongolia, a significant proportion of the general population had antibodies to hepatitis E virus (HEV). To investigate whether pigs are possible reservoirs of HEV in Mongolia, serum samples obtained from 243 2- or 3-month-old pigs on four swine farms surrounding Ulaanbaatar, the capital city of Mongolia, were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 223 pigs (91.8%) tested positive for anti-HEV, while 89 pigs (36.6%) had detectable HEV RNA. The 89 HEV isolates obtained from the viremic pigs were 78.7-100% identical to each other, and 80.9-85.9% similar to the prototype genotype 3 HEV isolate (US1) in the 412-nucleotide (nt) sequence within open reading frame 2. They were classified into two novel phylogenetic groups within genotype 3, differing by 16.4-21.3%. The swMN06-A1288 and swMN06-C1056 isolates, representing each of the two clusters within genotype 3, had a genomic length of nucleotides (nt) 7,222 nt and 7,223 nt, respectively, excluding the poly(A) tail, and shared only 81.6% over the entire genome. Upon comparison with the 25-reported genotype 3 HEV isolates over the entire genome, swMN06-A1288 had identities of merely up to 84.9%, while swMN06-C1056 of only up to 85.9%. Phylogenetic analysis confirmed the remote relatedness of the Mongolian swine isolates to the genotype 3 HEV isolates reported thus far. These results indicate that farm pigs in Mongolia are frequently infected with presumably indigenous HEV strains of genotype 3 and could be a source of HEV infections in humans in Mongolia. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/17597486/Analysis_of_the_full_length_genome_of_hepatitis_E_virus_isolates_obtained_from_farm_pigs_in_Mongolia_ L2 - https://doi.org/10.1002/jmv.20905 DB - PRIME DP - Unbound Medicine ER -