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Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees.
Vet Microbiol. 2007 Dec 15; 125(3-4):290-303.VM

Abstract

Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L) determinants. Four isolates (5%), which correlated with the Tc-resistant phenotypes, were found to carry the tet(K) determinant, whereas none carried the tet(L) determinant. P. larvae cells were also screened for the presence of extrachromosomal DNA and evidence obtained that tetracycline resistance is plasmid-encoded. A few P. larvae isolates were found to be able to transfer the tet(K) determinant to Bacillus subtilis, suggesting that a conjugation mechanism may be involved in the transfer of the tetracycline-resistant phenotype. Minimum inhibitory concentrations to tetracycline were determined for 75 isolates of P. larvae from different geographical origins and found to range between 0.062 and 128 microg tetracyclineml(-1), with MIC(50) and MIC(90) values of 1 and 4, respectively. According to results from P. larvae populations, isolates could be considered as susceptible when their MICs were <4, intermediate for MICs values 4-8 and resistant for MICs > or = 16. To our knowledge, this is the first report of Tc(r)Paenibacillus species carrying a tet(K) gene, and also the first record of P. larvae strains carrying tet(K) determinants and its correlation with the presence of extrachromosomal DNA.

Authors+Show Affiliations

Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Argentina. amalippi@netverk.com.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17601687

Citation

Alippi, Adriana M., et al. "Evidence for Plasmid-mediated Tetracycline Resistance in Paenibacillus Larvae, the Causal Agent of American Foulbrood (AFB) Disease in Honeybees." Veterinary Microbiology, vol. 125, no. 3-4, 2007, pp. 290-303.
Alippi AM, López AC, Reynaldi FJ, et al. Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. Vet Microbiol. 2007;125(3-4):290-303.
Alippi, A. M., López, A. C., Reynaldi, F. J., Grasso, D. H., & Aguilar, O. M. (2007). Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. Veterinary Microbiology, 125(3-4), 290-303.
Alippi AM, et al. Evidence for Plasmid-mediated Tetracycline Resistance in Paenibacillus Larvae, the Causal Agent of American Foulbrood (AFB) Disease in Honeybees. Vet Microbiol. 2007 Dec 15;125(3-4):290-303. PubMed PMID: 17601687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. AU - Alippi,Adriana M, AU - López,Ana C, AU - Reynaldi,Francisco J, AU - Grasso,Daniel H, AU - Aguilar,O Mario, Y1 - 2007/05/25/ PY - 2006/06/08/received PY - 2006/11/05/revised PY - 2007/05/16/accepted PY - 2007/7/3/pubmed PY - 2008/2/15/medline PY - 2007/7/3/entrez SP - 290 EP - 303 JF - Veterinary microbiology JO - Vet Microbiol VL - 125 IS - 3-4 N2 - Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L) determinants. Four isolates (5%), which correlated with the Tc-resistant phenotypes, were found to carry the tet(K) determinant, whereas none carried the tet(L) determinant. P. larvae cells were also screened for the presence of extrachromosomal DNA and evidence obtained that tetracycline resistance is plasmid-encoded. A few P. larvae isolates were found to be able to transfer the tet(K) determinant to Bacillus subtilis, suggesting that a conjugation mechanism may be involved in the transfer of the tetracycline-resistant phenotype. Minimum inhibitory concentrations to tetracycline were determined for 75 isolates of P. larvae from different geographical origins and found to range between 0.062 and 128 microg tetracyclineml(-1), with MIC(50) and MIC(90) values of 1 and 4, respectively. According to results from P. larvae populations, isolates could be considered as susceptible when their MICs were <4, intermediate for MICs values 4-8 and resistant for MICs > or = 16. To our knowledge, this is the first report of Tc(r)Paenibacillus species carrying a tet(K) gene, and also the first record of P. larvae strains carrying tet(K) determinants and its correlation with the presence of extrachromosomal DNA. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17601687/Evidence_for_plasmid_mediated_tetracycline_resistance_in_Paenibacillus_larvae_the_causal_agent_of_American_Foulbrood__AFB__disease_in_honeybees_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00263-5 DB - PRIME DP - Unbound Medicine ER -