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Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina.
Int J Biol Macromol. 2007 Oct 01; 41(4):369-75.IJ

Abstract

Since sulphated polysaccharides have antiviral activity in vitro, we examined the structure and antiretroviral activity of native sulphated galactans extracted from the red algae, Grateloupia filicina (GFP) and Grateloupia longifolia (GLP). The sulphate contents of GFP and GLPE (the 1,4-alpha-d-glucan-glucanohydrolase digest of GLP) were 25.7 and 18.5%, respectively. The sulphate ester groups were located at carbon 2 for GFP and at carbon 2 and 6 for GLPE. Antiretroviral activity was investigated with a primary isolate (PI) of HIV-1 and human peripheral blood mononuclear cells (PBMCs) rather than T-cell line adapted (TCLA) HIV-1 and T-cell lines because it is more representative of the in vivo situation. Both compounds and their derivatives had potent anti-HIV-1 activity when added at the time of infection, and 2h post-infection (EC50s 0.010-0.003microM, EC(90s) 0.87-0.33microM) and low cytotoxicity. Their potential medical application as virucidal vaginal formulations is discussed.

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Authors+Show Affiliations

Wang SC
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.
Bligh SW
No affiliation info available
Shi SS
No affiliation info available
Wang ZT
No affiliation info available
Hu ZB
No affiliation info available
Crowder J
No affiliation info available
Branford-White C
No affiliation info available
Vella C
No affiliation info available

MeSH

Antiviral AgentsCell SurvivalCells, CulturedChromatography, GelGalactansHIV-1Inhibitory Concentration 50Leukocytes, MononuclearMolecular WeightNuclear Magnetic Resonance, BiomolecularPolysaccharidesRhodophytaSpectrophotometry, InfraredSulfur CompoundsVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17602734

Citation

Wang, S C., et al. "Structural Features and anti-HIV-1 Activity of Novel Polysaccharides From Red Algae Grateloupia Longifolia and Grateloupia Filicina." International Journal of Biological Macromolecules, vol. 41, no. 4, 2007, pp. 369-75.
Wang SC, Bligh SW, Shi SS, et al. Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina. Int J Biol Macromol. 2007;41(4):369-75.
Wang, S. C., Bligh, S. W., Shi, S. S., Wang, Z. T., Hu, Z. B., Crowder, J., Branford-White, C., & Vella, C. (2007). Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina. International Journal of Biological Macromolecules, 41(4), 369-75.
Wang SC, et al. Structural Features and anti-HIV-1 Activity of Novel Polysaccharides From Red Algae Grateloupia Longifolia and Grateloupia Filicina. Int J Biol Macromol. 2007 Oct 1;41(4):369-75. PubMed PMID: 17602734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina. AU - Wang,S C, AU - Bligh,S W A, AU - Shi,S S, AU - Wang,Z T, AU - Hu,Z B, AU - Crowder,J, AU - Branford-White,C, AU - Vella,C, Y1 - 2007/05/29/ PY - 2007/03/02/received PY - 2007/05/08/revised PY - 2007/05/14/accepted PY - 2007/7/3/pubmed PY - 2007/11/8/medline PY - 2007/7/3/entrez SP - 369 EP - 75 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 41 IS - 4 N2 - Since sulphated polysaccharides have antiviral activity in vitro, we examined the structure and antiretroviral activity of native sulphated galactans extracted from the red algae, Grateloupia filicina (GFP) and Grateloupia longifolia (GLP). The sulphate contents of GFP and GLPE (the 1,4-alpha-d-glucan-glucanohydrolase digest of GLP) were 25.7 and 18.5%, respectively. The sulphate ester groups were located at carbon 2 for GFP and at carbon 2 and 6 for GLPE. Antiretroviral activity was investigated with a primary isolate (PI) of HIV-1 and human peripheral blood mononuclear cells (PBMCs) rather than T-cell line adapted (TCLA) HIV-1 and T-cell lines because it is more representative of the in vivo situation. Both compounds and their derivatives had potent anti-HIV-1 activity when added at the time of infection, and 2h post-infection (EC50s 0.010-0.003microM, EC(90s) 0.87-0.33microM) and low cytotoxicity. Their potential medical application as virucidal vaginal formulations is discussed. SN - 0141-8130 UR - https://www.unboundmedicine.com/medline/citation/17602734/Structural_features_and_anti_HIV_1_activity_of_novel_polysaccharides_from_red_algae_Grateloupia_longifolia_and_Grateloupia_filicina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(07)00135-3 DB - PRIME DP - Unbound Medicine ER -