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Simultaneous qualitation and quantification of thirteen bioactive compounds in Flos lonicerae by high-performance liquid chromatography with diode array detector and mass spectrometry.
Chem Pharm Bull (Tokyo). 2007 Jul; 55(7):1073-6.CP

Abstract

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of thirteen bioactive compounds in Flos Lonicerae. The optimal chromatographic conditions were obtained on a C(18) column (250x4.6 mm, 5.0 microm) with the column temperature at 30 degrees C. The mobile phase was composed of (A) acetic acid aqueous (0.4%, v/v) and (B) acetonitrile using a gradient elution, the flow rate was 1 ml/min. Detection wavelengths were set at 240 nm for iridoids (loganin, sweroside, secoxyloganin and centauroside), 330 nm for phenolic acids (chlorogenic acid, caffeic acid, 4,5-di-O-caffeoyl quinic acid and 3,4-di-O-caffeoyl quinic acid) and 360 nm for flavonoids (rutin, hyperoside, quercetin-3-O-glucoside, luteolin-7-O-glucoside and lonicerin). The identities of the peaks were accomplished by comparing retention times, UV and mass data with reference compounds under the same conditions. All calibration curves showed good linear regression (r(2)>0.9983) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.78--1.85% and 1.13--2.36%, respectively, and the overall recoveries of 91.3--104.2% for the thirteen compounds analyzed. The verified method was successfully applied to quantitative determination of the three types of bioactive compounds in ten commercial Flos Lonicerae samples from different markets in China. The analytical results demonstrated that the contents of the thirteen analytes were relatively variant.

Authors+Show Affiliations

Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17603205

Citation

Qian, Zheng-Ming, et al. "Simultaneous Qualitation and Quantification of Thirteen Bioactive Compounds in Flos Lonicerae By High-performance Liquid Chromatography With Diode Array Detector and Mass Spectrometry." Chemical & Pharmaceutical Bulletin, vol. 55, no. 7, 2007, pp. 1073-6.
Qian ZM, Li HJ, Li P, et al. Simultaneous qualitation and quantification of thirteen bioactive compounds in Flos lonicerae by high-performance liquid chromatography with diode array detector and mass spectrometry. Chem Pharm Bull (Tokyo). 2007;55(7):1073-6.
Qian, Z. M., Li, H. J., Li, P., Ren, M. T., & Tang, D. (2007). Simultaneous qualitation and quantification of thirteen bioactive compounds in Flos lonicerae by high-performance liquid chromatography with diode array detector and mass spectrometry. Chemical & Pharmaceutical Bulletin, 55(7), 1073-6.
Qian ZM, et al. Simultaneous Qualitation and Quantification of Thirteen Bioactive Compounds in Flos Lonicerae By High-performance Liquid Chromatography With Diode Array Detector and Mass Spectrometry. Chem Pharm Bull (Tokyo). 2007;55(7):1073-6. PubMed PMID: 17603205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous qualitation and quantification of thirteen bioactive compounds in Flos lonicerae by high-performance liquid chromatography with diode array detector and mass spectrometry. AU - Qian,Zheng-Ming, AU - Li,Hui-Jun, AU - Li,Ping, AU - Ren,Mei-Ting, AU - Tang,Dan, PY - 2007/7/3/pubmed PY - 2007/9/13/medline PY - 2007/7/3/entrez SP - 1073 EP - 6 JF - Chemical & pharmaceutical bulletin JO - Chem Pharm Bull (Tokyo) VL - 55 IS - 7 N2 - A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of thirteen bioactive compounds in Flos Lonicerae. The optimal chromatographic conditions were obtained on a C(18) column (250x4.6 mm, 5.0 microm) with the column temperature at 30 degrees C. The mobile phase was composed of (A) acetic acid aqueous (0.4%, v/v) and (B) acetonitrile using a gradient elution, the flow rate was 1 ml/min. Detection wavelengths were set at 240 nm for iridoids (loganin, sweroside, secoxyloganin and centauroside), 330 nm for phenolic acids (chlorogenic acid, caffeic acid, 4,5-di-O-caffeoyl quinic acid and 3,4-di-O-caffeoyl quinic acid) and 360 nm for flavonoids (rutin, hyperoside, quercetin-3-O-glucoside, luteolin-7-O-glucoside and lonicerin). The identities of the peaks were accomplished by comparing retention times, UV and mass data with reference compounds under the same conditions. All calibration curves showed good linear regression (r(2)>0.9983) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.78--1.85% and 1.13--2.36%, respectively, and the overall recoveries of 91.3--104.2% for the thirteen compounds analyzed. The verified method was successfully applied to quantitative determination of the three types of bioactive compounds in ten commercial Flos Lonicerae samples from different markets in China. The analytical results demonstrated that the contents of the thirteen analytes were relatively variant. SN - 0009-2363 UR - https://www.unboundmedicine.com/medline/citation/17603205/Simultaneous_qualitation_and_quantification_of_thirteen_bioactive_compounds_in_Flos_lonicerae_by_high_performance_liquid_chromatography_with_diode_array_detector_and_mass_spectrometry_ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/cpb/55.1073?from=PubMed DB - PRIME DP - Unbound Medicine ER -