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Using BOX-PCR to exclude a clonal outbreak of melioidosis.
BMC Infect Dis. 2007 Jun 30; 7:68.BI

Abstract

BACKGROUND

Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories.

METHODS

We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates.

RESULTS

BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters.

CONCLUSION

Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains.

Authors+Show Affiliations

Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia. bart@menzies.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17603903

Citation

Currie, Bart J., et al. "Using BOX-PCR to Exclude a Clonal Outbreak of Melioidosis." BMC Infectious Diseases, vol. 7, 2007, p. 68.
Currie BJ, Gal D, Mayo M, et al. Using BOX-PCR to exclude a clonal outbreak of melioidosis. BMC Infect Dis. 2007;7:68.
Currie, B. J., Gal, D., Mayo, M., Ward, L., Godoy, D., Spratt, B. G., & LiPuma, J. J. (2007). Using BOX-PCR to exclude a clonal outbreak of melioidosis. BMC Infectious Diseases, 7, 68.
Currie BJ, et al. Using BOX-PCR to Exclude a Clonal Outbreak of Melioidosis. BMC Infect Dis. 2007 Jun 30;7:68. PubMed PMID: 17603903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using BOX-PCR to exclude a clonal outbreak of melioidosis. AU - Currie,Bart J, AU - Gal,Daniel, AU - Mayo,Mark, AU - Ward,Linda, AU - Godoy,Daniel, AU - Spratt,Brian G, AU - LiPuma,John J, Y1 - 2007/06/30/ PY - 2007/04/18/received PY - 2007/06/30/accepted PY - 2007/7/3/pubmed PY - 2007/8/22/medline PY - 2007/7/3/entrez SP - 68 EP - 68 JF - BMC infectious diseases JO - BMC Infect Dis VL - 7 N2 - BACKGROUND: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. METHODS: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. RESULTS: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. CONCLUSION: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/17603903/Using_BOX_PCR_to_exclude_a_clonal_outbreak_of_melioidosis_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-7-68 DB - PRIME DP - Unbound Medicine ER -