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Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes.
Aquat Toxicol. 2007 Aug 15; 84(1):11-8.AT

Abstract

Little is known of the antioxidant role of selenium (Se) in aquatic invertebrates. We investigated the effects of Se on mercury-induced DNA damage in haemocytes from Mytilus edulis using alkaline single cell gel electrophoresis, that is, the Comet assay. The basal percentage tail DNA value for mussel haemocytes was 9.8+/-0.2% (mean+/-S.E.M., n=70). Exposing mussels to Hg(2+) (nominal concentration 20 microgL(-1)) for three days led to an increase in tail DNA to 61.1+/-1.8% (n=10). With added Se (as selenite, nominal concentration 4 microgL(-1)), Hg-induced DNA damage was reduced to 39.5+/-3.1% (n=10). Se pre-exposure also provided some protection against Hg-induced DNA damage (% tail DNA=51.0+/-2.9%, n=10). Basal glutathione peroxidase (GPx) activity in cell-free haemolymph was 93.7+/-3.5 nmol min(-1)mg(-1) (mean+/-S.E.M., n=70). Increases in GPx activity were seen when Se was added during and/or after exposure to Hg. For example, a 3-4-fold increase was seen after three days exposure to Hg in the presence of added Se. Interestingly GPx activity doubled after three days in the presence of added Se alone, but was unchanged after exposure to HgCl(2) alone. These results suggest that the availability of Se in the natural environment could affect the antioxidant status of mussels, and consequently could affect levels of DNA damage.

Authors+Show Affiliations

Ecotoxicology and Stress Biology Research Centre, School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon, PL4 8AA, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17604852

Citation

Tran, Damien, et al. "Protective Effects of Selenium On Mercury-induced DNA Damage in Mussel Haemocytes." Aquatic Toxicology (Amsterdam, Netherlands), vol. 84, no. 1, 2007, pp. 11-8.
Tran D, Moody AJ, Fisher AS, et al. Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes. Aquat Toxicol. 2007;84(1):11-8.
Tran, D., Moody, A. J., Fisher, A. S., Foulkes, M. E., & Jha, A. N. (2007). Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes. Aquatic Toxicology (Amsterdam, Netherlands), 84(1), 11-8.
Tran D, et al. Protective Effects of Selenium On Mercury-induced DNA Damage in Mussel Haemocytes. Aquat Toxicol. 2007 Aug 15;84(1):11-8. PubMed PMID: 17604852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes. AU - Tran,Damien, AU - Moody,A John, AU - Fisher,Andrew S, AU - Foulkes,Michael E, AU - Jha,Awadhesh N, Y1 - 2007/05/24/ PY - 2007/04/13/received PY - 2007/05/17/revised PY - 2007/05/17/accepted PY - 2007/7/3/pubmed PY - 2007/11/7/medline PY - 2007/7/3/entrez SP - 11 EP - 8 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 84 IS - 1 N2 - Little is known of the antioxidant role of selenium (Se) in aquatic invertebrates. We investigated the effects of Se on mercury-induced DNA damage in haemocytes from Mytilus edulis using alkaline single cell gel electrophoresis, that is, the Comet assay. The basal percentage tail DNA value for mussel haemocytes was 9.8+/-0.2% (mean+/-S.E.M., n=70). Exposing mussels to Hg(2+) (nominal concentration 20 microgL(-1)) for three days led to an increase in tail DNA to 61.1+/-1.8% (n=10). With added Se (as selenite, nominal concentration 4 microgL(-1)), Hg-induced DNA damage was reduced to 39.5+/-3.1% (n=10). Se pre-exposure also provided some protection against Hg-induced DNA damage (% tail DNA=51.0+/-2.9%, n=10). Basal glutathione peroxidase (GPx) activity in cell-free haemolymph was 93.7+/-3.5 nmol min(-1)mg(-1) (mean+/-S.E.M., n=70). Increases in GPx activity were seen when Se was added during and/or after exposure to Hg. For example, a 3-4-fold increase was seen after three days exposure to Hg in the presence of added Se. Interestingly GPx activity doubled after three days in the presence of added Se alone, but was unchanged after exposure to HgCl(2) alone. These results suggest that the availability of Se in the natural environment could affect the antioxidant status of mussels, and consequently could affect levels of DNA damage. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/17604852/Protective_effects_of_selenium_on_mercury_induced_DNA_damage_in_mussel_haemocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(07)00204-4 DB - PRIME DP - Unbound Medicine ER -