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[Expression of adhesion molecule CD44 on subpopulations of lymphocytes in hypertrophied adenoids in children with otitis media with effusion].
Otolaryngol Pol. 2007; 61(1):58-62.OP

Abstract

INTRODUCTION

The CD44 on lymphocytes binding to its ligand hyaluronan can mediate primary adhesion (rolling interactions) of lymphocytes on high endothelial venules (HEV). This adhesion molecule plays an important role to maturation and proliferation of lymphocytes in the secondary lymphoid organs. CD44 has a crucial role in migrate of lymphocytes to inflammatory sites. The AIM of this study was evaluation of the percentage and MFI (mean fluorescence intensity) of lymphocytes CD4+, CD8+, CD19+ with expression of surface adhesive molecule CD44 in hypertrophied adenoids in children with otitis media with effusion.

MATERIAL AND METHODS

42 children with otitis media with effusion and 30 children with hypertrophied adenoids were tested. Children were also divided into two subgroups: young (below 5 years) and older children (above 5 years old). Expression of adhesion molecule CD28 on lymphocytes of adenoids tissue was estimated by flow cytometry method.

RESULTS

This study showed significantly higher percentage of lymphocytes CD19+CD44+ in children with otitis media with effusion (OME 87,83%) than in comparative group with hypertrophied adenoids (AH 85,61%). We did not find difference between OME and AH in mean fluorescence intensity of subpopulations lymphocytes T and B with expression CD44.

CONCLUSIONS

Adhesion molecule CD44 is important for developing of effectors lymphocytes in hypertrophy adenoid. Increase percentage of lymphocytes CD19 with expression CD28, especially in older children confirms their participation in developing and forming of immunological response in otitis media with effusion.

Authors+Show Affiliations

Ratomski K
Zakład Laboratoryjnej Diagnostyki Pediatrycznej AM w Białymstoku. zldpam@poczta.fm
Zelazowska-Rutkowska B
No affiliation info available
Wysocka J
No affiliation info available
Skotnicka B
No affiliation info available

MeSH

AdenoidsAdolescentAntigens, CD19BiomarkersCD4 AntigensCD8 AntigensChildChild, PreschoolFemaleFlow CytometryHumansHyaluronan ReceptorsHypertrophyInfantMaleOtitis Media with EffusionSeverity of Illness IndexStatistics, NonparametricT-Lymphocytes

Pub Type(s)

Comparative Study
Journal Article

Language

pol

PubMed ID

17605420

Citation

Ratomski, Karol, et al. "[Expression of Adhesion Molecule CD44 On Subpopulations of Lymphocytes in Hypertrophied Adenoids in Children With Otitis Media With Effusion]." Otolaryngologia Polska = the Polish Otolaryngology, vol. 61, no. 1, 2007, pp. 58-62.
Ratomski K, Zelazowska-Rutkowska B, Wysocka J, et al. [Expression of adhesion molecule CD44 on subpopulations of lymphocytes in hypertrophied adenoids in children with otitis media with effusion]. Otolaryngol Pol. 2007;61(1):58-62.
Ratomski, K., Zelazowska-Rutkowska, B., Wysocka, J., & Skotnicka, B. (2007). [Expression of adhesion molecule CD44 on subpopulations of lymphocytes in hypertrophied adenoids in children with otitis media with effusion]. Otolaryngologia Polska = the Polish Otolaryngology, 61(1), 58-62.
Ratomski K, et al. [Expression of Adhesion Molecule CD44 On Subpopulations of Lymphocytes in Hypertrophied Adenoids in Children With Otitis Media With Effusion]. Otolaryngol Pol. 2007;61(1):58-62. PubMed PMID: 17605420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of adhesion molecule CD44 on subpopulations of lymphocytes in hypertrophied adenoids in children with otitis media with effusion]. AU - Ratomski,Karol, AU - Zelazowska-Rutkowska,Beata, AU - Wysocka,Jolanta, AU - Skotnicka,Bozena, PY - 2007/7/4/pubmed PY - 2007/8/31/medline PY - 2007/7/4/entrez SP - 58 EP - 62 JF - Otolaryngologia polska = The Polish otolaryngology JO - Otolaryngol Pol VL - 61 IS - 1 N2 - INTRODUCTION: The CD44 on lymphocytes binding to its ligand hyaluronan can mediate primary adhesion (rolling interactions) of lymphocytes on high endothelial venules (HEV). This adhesion molecule plays an important role to maturation and proliferation of lymphocytes in the secondary lymphoid organs. CD44 has a crucial role in migrate of lymphocytes to inflammatory sites. The AIM of this study was evaluation of the percentage and MFI (mean fluorescence intensity) of lymphocytes CD4+, CD8+, CD19+ with expression of surface adhesive molecule CD44 in hypertrophied adenoids in children with otitis media with effusion. MATERIAL AND METHODS: 42 children with otitis media with effusion and 30 children with hypertrophied adenoids were tested. Children were also divided into two subgroups: young (below 5 years) and older children (above 5 years old). Expression of adhesion molecule CD28 on lymphocytes of adenoids tissue was estimated by flow cytometry method. RESULTS: This study showed significantly higher percentage of lymphocytes CD19+CD44+ in children with otitis media with effusion (OME 87,83%) than in comparative group with hypertrophied adenoids (AH 85,61%). We did not find difference between OME and AH in mean fluorescence intensity of subpopulations lymphocytes T and B with expression CD44. CONCLUSIONS: Adhesion molecule CD44 is important for developing of effectors lymphocytes in hypertrophy adenoid. Increase percentage of lymphocytes CD19 with expression CD28, especially in older children confirms their participation in developing and forming of immunological response in otitis media with effusion. SN - 0030-6657 UR - https://www.unboundmedicine.com/medline/citation/17605420/[Expression_of_adhesion_molecule_CD44_on_subpopulations_of_lymphocytes_in_hypertrophied_adenoids_in_children_with_otitis_media_with_effusion]_ DB - PRIME DP - Unbound Medicine ER -