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Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection.
J Pharm Biomed Anal. 2007 Oct 18; 45(2):362-6.JP

Abstract

In this paper, a capillary electrophoresis (CE) system with in-column fiber optics light-emitting diode (LED) induced fluorescence detection was developed for the determination of penicillamine (PA). The influence of buffer concentration, buffer pH, applied voltage and injection time was systematically investigated. Optimum separation conditions were obtained with 10 mM borate buffer at pH 9.1, applied voltage 20 kV and 8 s hydrodynamic injection at 30 mbar. The detection system displayed linear dynamic range from 3.2 x 10(-7) to 4.8 x 10(-5) mol L(-1) with a correlation coefficient of 0.9991 and good repeatability (R.S.D.=2.46%). The method was applied to the determination of PA in commercial tablets and human plasma, which the recoveries of standard PA added to tablets and human plasma sample were found to be in the range of 96.26-102.68 and 91.10-99.35%, respectively. The proposed method is cheap, rapid, easy, and accurate, and can be successfully applied to the formulation analysis and bioanalysis.

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Authors+Show Affiliations

Yang X
College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
Yuan H
No affiliation info available
Wang C
No affiliation info available
Su X
No affiliation info available
Hu L
No affiliation info available
Xiao D
No affiliation info available

MeSH

Electrophoresis, CapillaryFiber Optic TechnologyHumansIndicators and ReagentsNaphthalenesOptical FibersPenicillaminePharmaceutical PreparationsReproducibility of ResultsSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17606355

Citation

Yang, Xiupei, et al. "Determination of Penicillamine in Pharmaceuticals and Human Plasma By Capillary Electrophoresis With In-column Fiber Optics Light-emitting Diode Induced Fluorescence Detection." Journal of Pharmaceutical and Biomedical Analysis, vol. 45, no. 2, 2007, pp. 362-6.
Yang X, Yuan H, Wang C, et al. Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection. J Pharm Biomed Anal. 2007;45(2):362-6.
Yang, X., Yuan, H., Wang, C., Su, X., Hu, L., & Xiao, D. (2007). Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection. Journal of Pharmaceutical and Biomedical Analysis, 45(2), 362-6.
Yang X, et al. Determination of Penicillamine in Pharmaceuticals and Human Plasma By Capillary Electrophoresis With In-column Fiber Optics Light-emitting Diode Induced Fluorescence Detection. J Pharm Biomed Anal. 2007 Oct 18;45(2):362-6. PubMed PMID: 17606355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection. AU - Yang,Xiupei, AU - Yuan,Hongyan, AU - Wang,Chunling, AU - Su,Xiaodong, AU - Hu,Li, AU - Xiao,Dan, Y1 - 2007/05/25/ PY - 2007/02/11/received PY - 2007/04/18/revised PY - 2007/05/20/accepted PY - 2007/7/4/pubmed PY - 2007/12/14/medline PY - 2007/7/4/entrez SP - 362 EP - 6 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 45 IS - 2 N2 - In this paper, a capillary electrophoresis (CE) system with in-column fiber optics light-emitting diode (LED) induced fluorescence detection was developed for the determination of penicillamine (PA). The influence of buffer concentration, buffer pH, applied voltage and injection time was systematically investigated. Optimum separation conditions were obtained with 10 mM borate buffer at pH 9.1, applied voltage 20 kV and 8 s hydrodynamic injection at 30 mbar. The detection system displayed linear dynamic range from 3.2 x 10(-7) to 4.8 x 10(-5) mol L(-1) with a correlation coefficient of 0.9991 and good repeatability (R.S.D.=2.46%). The method was applied to the determination of PA in commercial tablets and human plasma, which the recoveries of standard PA added to tablets and human plasma sample were found to be in the range of 96.26-102.68 and 91.10-99.35%, respectively. The proposed method is cheap, rapid, easy, and accurate, and can be successfully applied to the formulation analysis and bioanalysis. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17606355/Determination_of_penicillamine_in_pharmaceuticals_and_human_plasma_by_capillary_electrophoresis_with_in_column_fiber_optics_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(07)00277-4 DB - PRIME DP - Unbound Medicine ER -