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Determination of norfloxacin in rat liver perfusate using capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 01; 856(1-2):381-5.JC

Abstract

A capillary zone electrophoresis method has been developed for the direct determination of norfloxacin in the physiological perfusate of isolated rat liver. Norfloxacin and the internal standard triamterene were detected using laser-induced fluorescence (LIF) detection with the excitation and emission wavelength of 325 and 435 nm, respectively. The background electrolyte (BGE) was 50 mM phosphate buffer (pH 4.6). The effect of pH and concentration of BGE on the electrophoretic migration and fluorescence response of analytes were examined. Calibration curves were linear over a wide range of 0.01-100 microg/mL. The limit of quantitation was 0.01 microg/mL. The intra- and inter-day relative standard deviation was 3.7%, or less, and the accuracy was 93.2% of the nominal concentration. No endogenous substances were found to interfere. The method was used to characterize the steady-state and transient pharmacokinetics of norfloxacin in the rat liver.

Authors+Show Affiliations

Department of Pharmacy, Chia-Nan University of Pharmacy and Science, No. 60, Section 1, Erh-Jen Road, Jen-Te, Tainan 71710, Taiwan. hccl@mail.chna.edu.twNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17606416

Citation

Cheng, Ching-Ling, et al. "Determination of Norfloxacin in Rat Liver Perfusate Using Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 856, no. 1-2, 2007, pp. 381-5.
Cheng CL, Fu CH, Chou CH. Determination of norfloxacin in rat liver perfusate using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;856(1-2):381-5.
Cheng, C. L., Fu, C. H., & Chou, C. H. (2007). Determination of norfloxacin in rat liver perfusate using capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 856(1-2), 381-5.
Cheng CL, Fu CH, Chou CH. Determination of Norfloxacin in Rat Liver Perfusate Using Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):381-5. PubMed PMID: 17606416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of norfloxacin in rat liver perfusate using capillary electrophoresis with laser-induced fluorescence detection. AU - Cheng,Ching-Ling, AU - Fu,Chia-Hung, AU - Chou,Chen-Hsi, Y1 - 2007/06/22/ PY - 2007/02/27/received PY - 2007/06/10/revised PY - 2007/06/17/accepted PY - 2007/7/4/pubmed PY - 2007/12/6/medline PY - 2007/7/4/entrez SP - 381 EP - 5 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 856 IS - 1-2 N2 - A capillary zone electrophoresis method has been developed for the direct determination of norfloxacin in the physiological perfusate of isolated rat liver. Norfloxacin and the internal standard triamterene were detected using laser-induced fluorescence (LIF) detection with the excitation and emission wavelength of 325 and 435 nm, respectively. The background electrolyte (BGE) was 50 mM phosphate buffer (pH 4.6). The effect of pH and concentration of BGE on the electrophoretic migration and fluorescence response of analytes were examined. Calibration curves were linear over a wide range of 0.01-100 microg/mL. The limit of quantitation was 0.01 microg/mL. The intra- and inter-day relative standard deviation was 3.7%, or less, and the accuracy was 93.2% of the nominal concentration. No endogenous substances were found to interfere. The method was used to characterize the steady-state and transient pharmacokinetics of norfloxacin in the rat liver. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17606416/Determination_of_norfloxacin_in_rat_liver_perfusate_using_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00435-7 DB - PRIME DP - Unbound Medicine ER -