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Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection.
Singapore Med J. 2007 Jul; 48(7):669-73.SM

Abstract

INTRODUCTION

The aim of this report is to establish an accurate diagnosis of acute dengue virus infection early, in order to provide timely information for the management of patients and early public health control of dengue outbreak.

METHODS

224 serum samples from patients with a clinical diagnosis of acute dengue infection, which were subsequently confirmed by laboratory tests, were used to evaluate the performance of a commercially-available dengue NS1 antigen-capture ELISA kit.

RESULTS

The dengue NS1 antigen-capture ELISA gave an overall sensitivity rate of 93.3 percent (209/224). The sensitivity rate was significantly higher in acute primary dengue (97.4 percent) than in acute secondary dengue (68.8 percent). In comparison, the virus isolation gave an overall positive isolation rate of 64.7 percent, with a positive rate of 70.8 percent and 28.1 percent, for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 63.4 percent, with a positive rate of 62.5 percent and 68.8 percent, for acute primary dengue and acute secondary dengue, respectively. Of the 224 acute serum samples from patients with laboratory-confirmed acute dengue infection, dengue IgM was detected in 88 specimens, comprising 68 acute primary dengue specimens and 20 acute secondary dengue specimens. NS1 antigen-capture ELISA kit gave an overall sensitivity rate of 88.6 percent in the presence of anti-dengue IgM and 96.3 percent in the absence of anti-dengue IgM.

CONCLUSION

Of the 224 acute serum samples, the sample ages of 166 acute serum samples are known. The positive detection rate of dengue NS1 antigen-capture ELISA, on the whole, was higher than the other three established diagnostic test methods for laboratory diagnosis of acute dengue infection.

Authors+Show Affiliations

The National Public Health Laboratory, Ministry of Health, Lot 1853 Kg Melayu, Sungai Buloh 47000, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17609831

Citation

Kumarasamy, V, et al. "Evaluating the Sensitivity of a Commercial Dengue NS1 Antigen-capture ELISA for Early Diagnosis of Acute Dengue Virus Infection." Singapore Medical Journal, vol. 48, no. 7, 2007, pp. 669-73.
Kumarasamy V, Chua SK, Hassan Z, et al. Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. Singapore Med J. 2007;48(7):669-73.
Kumarasamy, V., Chua, S. K., Hassan, Z., Wahab, A. H., Chem, Y. K., Mohamad, M., & Chua, K. B. (2007). Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. Singapore Medical Journal, 48(7), 669-73.
Kumarasamy V, et al. Evaluating the Sensitivity of a Commercial Dengue NS1 Antigen-capture ELISA for Early Diagnosis of Acute Dengue Virus Infection. Singapore Med J. 2007;48(7):669-73. PubMed PMID: 17609831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. AU - Kumarasamy,V, AU - Chua,S K, AU - Hassan,Z, AU - Wahab,A H A, AU - Chem,Y K, AU - Mohamad,M, AU - Chua,K B, PY - 2007/7/5/pubmed PY - 2007/8/1/medline PY - 2007/7/5/entrez SP - 669 EP - 73 JF - Singapore medical journal JO - Singapore Med J VL - 48 IS - 7 N2 - INTRODUCTION: The aim of this report is to establish an accurate diagnosis of acute dengue virus infection early, in order to provide timely information for the management of patients and early public health control of dengue outbreak. METHODS: 224 serum samples from patients with a clinical diagnosis of acute dengue infection, which were subsequently confirmed by laboratory tests, were used to evaluate the performance of a commercially-available dengue NS1 antigen-capture ELISA kit. RESULTS: The dengue NS1 antigen-capture ELISA gave an overall sensitivity rate of 93.3 percent (209/224). The sensitivity rate was significantly higher in acute primary dengue (97.4 percent) than in acute secondary dengue (68.8 percent). In comparison, the virus isolation gave an overall positive isolation rate of 64.7 percent, with a positive rate of 70.8 percent and 28.1 percent, for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 63.4 percent, with a positive rate of 62.5 percent and 68.8 percent, for acute primary dengue and acute secondary dengue, respectively. Of the 224 acute serum samples from patients with laboratory-confirmed acute dengue infection, dengue IgM was detected in 88 specimens, comprising 68 acute primary dengue specimens and 20 acute secondary dengue specimens. NS1 antigen-capture ELISA kit gave an overall sensitivity rate of 88.6 percent in the presence of anti-dengue IgM and 96.3 percent in the absence of anti-dengue IgM. CONCLUSION: Of the 224 acute serum samples, the sample ages of 166 acute serum samples are known. The positive detection rate of dengue NS1 antigen-capture ELISA, on the whole, was higher than the other three established diagnostic test methods for laboratory diagnosis of acute dengue infection. SN - 0037-5675 UR - https://www.unboundmedicine.com/medline/citation/17609831/Evaluating_the_sensitivity_of_a_commercial_dengue_NS1_antigen_capture_ELISA_for_early_diagnosis_of_acute_dengue_virus_infection_ L2 - http://smj.sma.org.sg/4807/4807a12.pdf DB - PRIME DP - Unbound Medicine ER -