Tags

Type your tag names separated by a space and hit enter

Surface-activated chemical ionization ion trap mass spectrometry for the analysis of cocaine and benzoylecgonine in hair after extraction and sample dilution.
Rapid Commun Mass Spectrom. 2007; 21(15):2515-23.RC

Abstract

Surface-activated chemical ionization (SACI) was employed for the analysis of cocaine and its metabolite, benzoylecgonine, extracted from hair. Following decontamination and acid hydrolysis procedures on the hair sample, the sample solution was diluted (1:10) and directly analyzed by liquid chromatography/surface-activated chemical ionization multiple collisional stage single reaction monitoring mass spectrometry (LC/SACI-MS(3)-SRM) without solid-phase extraction (SPE) pre-purification and concentration procedures. To increase the selectivity of the method, MS(3) was chosen instead of the less selective MS/MS. This data was compared with that achieved using gas chromatography/mass spectrometry (GC/MS), the reference method used by the Italian Government Institute of Health protocol. The limits of detection (LODs) were 0.003 ng/(mg hair) for cocaine and 0.02 ng/(mg hair) for benzoylecgonine and the limits of quantitation (LOQs) were 0.01 ng/(mg hair) for cocaine and 0.04 ng/(mg hair) for benzoylecgonine. The squared correlation coefficient (R(2)) of the calibration curve was 0.9887-0.9980 for cocaine and 0.9987-0.9997 for benzoylecgonine. The percent accuracy error was 2-5% for both cocaine and benzoylecgonine using the LC/SACI-MS(3)-SRM approach, whereas it was higher for benzoylecgonine (20-25%) using the LC/SACI-MS/MS-SRM approach compared with the GC/MS data due to hair matrix contamination. In both cases, high precision was achieved (1-3% precision error), which confirmed the stability of the developed methods.

Authors+Show Affiliations

ISB - Ion Source & Biotechnologies, Milano, Italy. simone.cristoni@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Validation Study

Language

eng

PubMed ID

17610217

Citation

Cristoni, Simone, et al. "Surface-activated Chemical Ionization Ion Trap Mass Spectrometry for the Analysis of Cocaine and Benzoylecgonine in Hair After Extraction and Sample Dilution." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 15, 2007, pp. 2515-23.
Cristoni S, Basso E, Gerthoux P, et al. Surface-activated chemical ionization ion trap mass spectrometry for the analysis of cocaine and benzoylecgonine in hair after extraction and sample dilution. Rapid Commun Mass Spectrom. 2007;21(15):2515-23.
Cristoni, S., Basso, E., Gerthoux, P., Mocarelli, P., Gonella, E., Brambilla, M., Crotti, S., & Bernardi, L. R. (2007). Surface-activated chemical ionization ion trap mass spectrometry for the analysis of cocaine and benzoylecgonine in hair after extraction and sample dilution. Rapid Communications in Mass Spectrometry : RCM, 21(15), 2515-23.
Cristoni S, et al. Surface-activated Chemical Ionization Ion Trap Mass Spectrometry for the Analysis of Cocaine and Benzoylecgonine in Hair After Extraction and Sample Dilution. Rapid Commun Mass Spectrom. 2007;21(15):2515-23. PubMed PMID: 17610217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-activated chemical ionization ion trap mass spectrometry for the analysis of cocaine and benzoylecgonine in hair after extraction and sample dilution. AU - Cristoni,Simone, AU - Basso,Elisa, AU - Gerthoux,Piermario, AU - Mocarelli,Paolo, AU - Gonella,Elisabetta, AU - Brambilla,Maura, AU - Crotti,Sara, AU - Bernardi,Luigi Rossi, PY - 2007/7/5/pubmed PY - 2007/10/30/medline PY - 2007/7/5/entrez SP - 2515 EP - 23 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 15 N2 - Surface-activated chemical ionization (SACI) was employed for the analysis of cocaine and its metabolite, benzoylecgonine, extracted from hair. Following decontamination and acid hydrolysis procedures on the hair sample, the sample solution was diluted (1:10) and directly analyzed by liquid chromatography/surface-activated chemical ionization multiple collisional stage single reaction monitoring mass spectrometry (LC/SACI-MS(3)-SRM) without solid-phase extraction (SPE) pre-purification and concentration procedures. To increase the selectivity of the method, MS(3) was chosen instead of the less selective MS/MS. This data was compared with that achieved using gas chromatography/mass spectrometry (GC/MS), the reference method used by the Italian Government Institute of Health protocol. The limits of detection (LODs) were 0.003 ng/(mg hair) for cocaine and 0.02 ng/(mg hair) for benzoylecgonine and the limits of quantitation (LOQs) were 0.01 ng/(mg hair) for cocaine and 0.04 ng/(mg hair) for benzoylecgonine. The squared correlation coefficient (R(2)) of the calibration curve was 0.9887-0.9980 for cocaine and 0.9987-0.9997 for benzoylecgonine. The percent accuracy error was 2-5% for both cocaine and benzoylecgonine using the LC/SACI-MS(3)-SRM approach, whereas it was higher for benzoylecgonine (20-25%) using the LC/SACI-MS/MS-SRM approach compared with the GC/MS data due to hair matrix contamination. In both cases, high precision was achieved (1-3% precision error), which confirmed the stability of the developed methods. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17610217/Surface_activated_chemical_ionization_ion_trap_mass_spectrometry_for_the_analysis_of_cocaine_and_benzoylecgonine_in_hair_after_extraction_and_sample_dilution_ L2 - https://doi.org/10.1002/rcm.3107 DB - PRIME DP - Unbound Medicine ER -