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Generation of a conditional Ppap2b/Lpp3 null allele.
Genesis. 2007 Jul; 45(7):465-9.G

Abstract

Lpp3, formerly known as Pap2b, is a lipid phosphohydrolase enzyme. Some of its substrates and products are lipids with potent biological and signaling activities such as phosphatidic acid, lysophosphatidic acid, sphingosine-1-phosphate, diacylglycerol, sphingosine, and ceramide. Lpp3 is dynamically expressed during development and is widely distributed in adult tissues. Targeted inactivation of Lpp3 gene (Ppap2b) in the mouse results in embryonic lethality because of defects in extraembryonic vascular development and gastrulation. To study the participation of Lpp3 later in development and in specific cell lineages we generated a conditional allele of Ppap2b. This was accomplished by flanking critical exons, responsible for its catalytic activity with loxP sites. A generalized Cre-mediated recombination of this allele yielded a phenotype fundamentally identical to our previously reported Ppap2b null allele.

Authors+Show Affiliations

Cancer and Developmental Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17610274

Citation

Escalante-Alcalde, Diana, et al. "Generation of a Conditional Ppap2b/Lpp3 Null Allele." Genesis (New York, N.Y. : 2000), vol. 45, no. 7, 2007, pp. 465-9.
Escalante-Alcalde D, Sánchez-Sánchez R, Stewart CL. Generation of a conditional Ppap2b/Lpp3 null allele. Genesis. 2007;45(7):465-9.
Escalante-Alcalde, D., Sánchez-Sánchez, R., & Stewart, C. L. (2007). Generation of a conditional Ppap2b/Lpp3 null allele. Genesis (New York, N.Y. : 2000), 45(7), 465-9.
Escalante-Alcalde D, Sánchez-Sánchez R, Stewart CL. Generation of a Conditional Ppap2b/Lpp3 Null Allele. Genesis. 2007;45(7):465-9. PubMed PMID: 17610274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a conditional Ppap2b/Lpp3 null allele. AU - Escalante-Alcalde,Diana, AU - Sánchez-Sánchez,Roberto, AU - Stewart,Colin L, PY - 2007/7/5/pubmed PY - 2007/9/20/medline PY - 2007/7/5/entrez SP - 465 EP - 9 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 45 IS - 7 N2 - Lpp3, formerly known as Pap2b, is a lipid phosphohydrolase enzyme. Some of its substrates and products are lipids with potent biological and signaling activities such as phosphatidic acid, lysophosphatidic acid, sphingosine-1-phosphate, diacylglycerol, sphingosine, and ceramide. Lpp3 is dynamically expressed during development and is widely distributed in adult tissues. Targeted inactivation of Lpp3 gene (Ppap2b) in the mouse results in embryonic lethality because of defects in extraembryonic vascular development and gastrulation. To study the participation of Lpp3 later in development and in specific cell lineages we generated a conditional allele of Ppap2b. This was accomplished by flanking critical exons, responsible for its catalytic activity with loxP sites. A generalized Cre-mediated recombination of this allele yielded a phenotype fundamentally identical to our previously reported Ppap2b null allele. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/17610274/Generation_of_a_conditional_Ppap2b/Lpp3_null_allele_ L2 - https://doi.org/10.1002/dvg.20314 DB - PRIME DP - Unbound Medicine ER -