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Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs.
Vet Microbiol. 2007 Dec 15; 125(3-4):244-55.VM

Abstract

The notion that postweaning multisystemic wasting syndrome (PMWS)-affected pigs develop an impaired humoral response against porcine circovirus type 2 (PCV2) has been reported in several studies. However, little information is available regarding the presence of neutralizing antibodies (NA) in PCV2-infected pigs and their role in the pathogenesis of the disease. The aim of the present work was to further characterize the humoral response, and in particular the production of NA, in pigs with different PCV2-infection status. Seventy-two conventional pigs from different farms were classified into three groups based on PCV2 infection and clinico-pathological status, namely: PCV2-negative, non-PMWS PCV2-positive and PMWS-affected animals. In addition, 9-week old pigs from an experimental infection (6 controls and 14 PCV2-inoculated pigs) were also studied. NA and total PCV2 antibodies (TA) as well as viral load in serum were determined and correlated with the clinico-pathological status of pigs. Results indicated that PMWS-affected pigs had lower NA titres, if any, than healthy animals. NA titres were also inversely correlated with PCV2 load in serum. NA and TA titres were positively correlated; however, correlation differed among infection status, being lower in PCV2-positive pigs. Also, the diagnostic performance of each test was evaluated, indicating that the combination of viral neutralization and quantitative PCR in serum was useful to discard PMWS (specificity 92%). In experimentally infected animals, the evolution of NA paralleled the course TA, although a slight delay in NA production was seen in some animals. The increase of NA coincided with the drop in viral load. Results from this work further support that PMWS-affected pigs show an impaired humoral immune response and, particularly, an inefficient NA response against PCV2.

Authors+Show Affiliations

Centre de Recerca en Sanitat Animal (CReSA), Esfera UAB, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. maria.fort@cresa.uab.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17611048

Citation

Fort, Maria, et al. "Detection of Neutralizing Antibodies in Postweaning Multisystemic Wasting Syndrome (PMWS)-affected and non-PMWS-affected Pigs." Veterinary Microbiology, vol. 125, no. 3-4, 2007, pp. 244-55.
Fort M, Olvera A, Sibila M, et al. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet Microbiol. 2007;125(3-4):244-55.
Fort, M., Olvera, A., Sibila, M., Segalés, J., & Mateu, E. (2007). Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Veterinary Microbiology, 125(3-4), 244-55.
Fort M, et al. Detection of Neutralizing Antibodies in Postweaning Multisystemic Wasting Syndrome (PMWS)-affected and non-PMWS-affected Pigs. Vet Microbiol. 2007 Dec 15;125(3-4):244-55. PubMed PMID: 17611048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. AU - Fort,Maria, AU - Olvera,Alex, AU - Sibila,Marina, AU - Segalés,Joaquim, AU - Mateu,Enric, Y1 - 2007/06/13/ PY - 2007/01/02/received PY - 2007/06/01/revised PY - 2007/06/05/accepted PY - 2007/7/6/pubmed PY - 2008/2/15/medline PY - 2007/7/6/entrez SP - 244 EP - 55 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 125 IS - 3-4 N2 - The notion that postweaning multisystemic wasting syndrome (PMWS)-affected pigs develop an impaired humoral response against porcine circovirus type 2 (PCV2) has been reported in several studies. However, little information is available regarding the presence of neutralizing antibodies (NA) in PCV2-infected pigs and their role in the pathogenesis of the disease. The aim of the present work was to further characterize the humoral response, and in particular the production of NA, in pigs with different PCV2-infection status. Seventy-two conventional pigs from different farms were classified into three groups based on PCV2 infection and clinico-pathological status, namely: PCV2-negative, non-PMWS PCV2-positive and PMWS-affected animals. In addition, 9-week old pigs from an experimental infection (6 controls and 14 PCV2-inoculated pigs) were also studied. NA and total PCV2 antibodies (TA) as well as viral load in serum were determined and correlated with the clinico-pathological status of pigs. Results indicated that PMWS-affected pigs had lower NA titres, if any, than healthy animals. NA titres were also inversely correlated with PCV2 load in serum. NA and TA titres were positively correlated; however, correlation differed among infection status, being lower in PCV2-positive pigs. Also, the diagnostic performance of each test was evaluated, indicating that the combination of viral neutralization and quantitative PCR in serum was useful to discard PMWS (specificity 92%). In experimentally infected animals, the evolution of NA paralleled the course TA, although a slight delay in NA production was seen in some animals. The increase of NA coincided with the drop in viral load. Results from this work further support that PMWS-affected pigs show an impaired humoral immune response and, particularly, an inefficient NA response against PCV2. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17611048/Detection_of_neutralizing_antibodies_in_postweaning_multisystemic_wasting_syndrome__PMWS__affected_and_non_PMWS_affected_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00296-9 DB - PRIME DP - Unbound Medicine ER -