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Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects.
Clin Exp Immunol. 2007 Oct; 150(1):22-9.CE

Abstract

The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T cells obtained by bronchial brushing (BB). Intracellular cytokine analysis of T cell subsets from peripheral blood, BAL and BB from smoker and ex-smoker COPD patients, COPD patients receiving inhaled corticosteroids and smoker and non-smoker control subjects was studied using multi-parameter flow cytometry. CD4 : CD8 inversion was noted in the peripheral blood of smoker and ex-smoker COPD groups, in BAL and BB from smoker controls and BAL of COPD smokers. There was an increase in intracellular CD8(+) T cell Th1 proinflammatory cytokines in some COPD groups in the peripheral blood and in CD8(+) T cell tumour necrosis factor (TNF)-alpha in some COPD groups and smoker controls in BAL and BB. There was an increase in proinflammatory cytokines in COPD smokers compared with ex-smokers and a decrease in COPD smokers receiving inhaled corticosteroids in the airways. There was a negative correlation between forced expiratory volume in 1 s (FEV(1)) and the percentage of BAL and intraepithelial CD8(+) T cells producing TNF-alpha. COPD patients exhibit systemic inflammation as evidenced by increased intracellular Th1 proinflammatory cytokines in blood, BAL and intraepithelial CD8(+) T cells, whereas smoker controls showed localized Th1 response in the lung only. Systemic therapeutic targeting of TNF-alpha production by CD8(+) T cells may improve morbidity in COPD patients while targeting of TNF-alpha in the lung may prevent smokers progressing to COPD.

Authors+Show Affiliations

Department of Thoracic Medicine, Royal Adelaide Hospital, Australia. greg.hodge@cywhs.sa.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17614970

Citation

Hodge, G, et al. "Increased Intracellular T Helper 1 Proinflammatory Cytokine Production in Peripheral Blood, Bronchoalveolar Lavage and Intraepithelial T Cells of COPD Subjects." Clinical and Experimental Immunology, vol. 150, no. 1, 2007, pp. 22-9.
Hodge G, Nairn J, Holmes M, et al. Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects. Clin Exp Immunol. 2007;150(1):22-9.
Hodge, G., Nairn, J., Holmes, M., Reynolds, P. N., & Hodge, S. (2007). Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects. Clinical and Experimental Immunology, 150(1), 22-9.
Hodge G, et al. Increased Intracellular T Helper 1 Proinflammatory Cytokine Production in Peripheral Blood, Bronchoalveolar Lavage and Intraepithelial T Cells of COPD Subjects. Clin Exp Immunol. 2007;150(1):22-9. PubMed PMID: 17614970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects. AU - Hodge,G, AU - Nairn,J, AU - Holmes,M, AU - Reynolds,P N, AU - Hodge,S, Y1 - 2007/07/05/ PY - 2007/7/7/pubmed PY - 2007/11/9/medline PY - 2007/7/7/entrez SP - 22 EP - 9 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 150 IS - 1 N2 - The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T cells obtained by bronchial brushing (BB). Intracellular cytokine analysis of T cell subsets from peripheral blood, BAL and BB from smoker and ex-smoker COPD patients, COPD patients receiving inhaled corticosteroids and smoker and non-smoker control subjects was studied using multi-parameter flow cytometry. CD4 : CD8 inversion was noted in the peripheral blood of smoker and ex-smoker COPD groups, in BAL and BB from smoker controls and BAL of COPD smokers. There was an increase in intracellular CD8(+) T cell Th1 proinflammatory cytokines in some COPD groups in the peripheral blood and in CD8(+) T cell tumour necrosis factor (TNF)-alpha in some COPD groups and smoker controls in BAL and BB. There was an increase in proinflammatory cytokines in COPD smokers compared with ex-smokers and a decrease in COPD smokers receiving inhaled corticosteroids in the airways. There was a negative correlation between forced expiratory volume in 1 s (FEV(1)) and the percentage of BAL and intraepithelial CD8(+) T cells producing TNF-alpha. COPD patients exhibit systemic inflammation as evidenced by increased intracellular Th1 proinflammatory cytokines in blood, BAL and intraepithelial CD8(+) T cells, whereas smoker controls showed localized Th1 response in the lung only. Systemic therapeutic targeting of TNF-alpha production by CD8(+) T cells may improve morbidity in COPD patients while targeting of TNF-alpha in the lung may prevent smokers progressing to COPD. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/17614970/Increased_intracellular_T_helper_1_proinflammatory_cytokine_production_in_peripheral_blood_bronchoalveolar_lavage_and_intraepithelial_T_cells_of_COPD_subjects_ L2 - https://doi.org/10.1111/j.1365-2249.2007.03451.x DB - PRIME DP - Unbound Medicine ER -