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The epitope study of alpha-fodrin autoantibody in primary Sjögren's syndrome.
Clin Exp Immunol. 2007 Sep; 149(3):497-503.CE

Abstract

Alpha-fodrin, an intracellular organ-specific cytoskeleton protein, was identified recently as an autoantigen associated with Sicca- and Sjögren's syndrome (SS). Identification of the antigenic determinants of alpha-fodrin is a prerequisite to developing highly sensitive and specific anti-alpha-fodrin antibodies, which provides potential means for the diagnosis of primary Sjögren's syndrome (pSS) in patients. Based on the structure and predicted antigenic sites of alpha-fodrin protein with 560 amino acids (alpha-fodrin 560), we prepared a set of overlapping recombinant protein fragments covering antigenic epitopes and synthesized a set of peptides derived from the alpha-fodrin protein. These recombinant proteins and synthesized peptides were subjected to screening with pSS patients sera, respectively. The peptide with the strongest immunoreactivity was used as antigenic peptide to define further the role of anti-alpha-fodrin-peptide antibodies in the sera of 135 patients with pSS, 48 patients with systemic lupus erythematosus (SLE), 88 patients with rheumatoid arthritis (RA) and 83 normal controls. Our data showed that the N-terminal peptide of amino acids 46-59 (N46) of alpha-fodrin 560 was the epitope with strongest antigenicity. The prevalences of anti-N46 peptide antibodies (alpha-N46PA) in patients with pSS, SLE, RA and normal controls were 78.5%, 10.4%, 21.6% and 6.0%, respectively. The sensitivity and specificity of the autoantibodies in pSS were 78.5% and 86.8%, respectively. These results suggest the alpha-N46PA which shows highest sensitivity and specificity is of significance to develop an effective diagnostic approach for pSS.

Authors+Show Affiliations

Laboratory of Molecular Diagnostics, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17614976

Citation

Chen, Q, et al. "The Epitope Study of Alpha-fodrin Autoantibody in Primary Sjögren's Syndrome." Clinical and Experimental Immunology, vol. 149, no. 3, 2007, pp. 497-503.
Chen Q, Li X, He W, et al. The epitope study of alpha-fodrin autoantibody in primary Sjögren's syndrome. Clin Exp Immunol. 2007;149(3):497-503.
Chen, Q., Li, X., He, W., Zhang, H., Gao, A., Cheng, Y., Lei, J., Li, S., & Zeng, L. (2007). The epitope study of alpha-fodrin autoantibody in primary Sjögren's syndrome. Clinical and Experimental Immunology, 149(3), 497-503.
Chen Q, et al. The Epitope Study of Alpha-fodrin Autoantibody in Primary Sjögren's Syndrome. Clin Exp Immunol. 2007;149(3):497-503. PubMed PMID: 17614976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The epitope study of alpha-fodrin autoantibody in primary Sjögren's syndrome. AU - Chen,Q, AU - Li,X, AU - He,W, AU - Zhang,H, AU - Gao,A, AU - Cheng,Y, AU - Lei,J, AU - Li,S, AU - Zeng,L, Y1 - 2007/07/05/ PY - 2007/7/7/pubmed PY - 2007/10/27/medline PY - 2007/7/7/entrez SP - 497 EP - 503 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 149 IS - 3 N2 - Alpha-fodrin, an intracellular organ-specific cytoskeleton protein, was identified recently as an autoantigen associated with Sicca- and Sjögren's syndrome (SS). Identification of the antigenic determinants of alpha-fodrin is a prerequisite to developing highly sensitive and specific anti-alpha-fodrin antibodies, which provides potential means for the diagnosis of primary Sjögren's syndrome (pSS) in patients. Based on the structure and predicted antigenic sites of alpha-fodrin protein with 560 amino acids (alpha-fodrin 560), we prepared a set of overlapping recombinant protein fragments covering antigenic epitopes and synthesized a set of peptides derived from the alpha-fodrin protein. These recombinant proteins and synthesized peptides were subjected to screening with pSS patients sera, respectively. The peptide with the strongest immunoreactivity was used as antigenic peptide to define further the role of anti-alpha-fodrin-peptide antibodies in the sera of 135 patients with pSS, 48 patients with systemic lupus erythematosus (SLE), 88 patients with rheumatoid arthritis (RA) and 83 normal controls. Our data showed that the N-terminal peptide of amino acids 46-59 (N46) of alpha-fodrin 560 was the epitope with strongest antigenicity. The prevalences of anti-N46 peptide antibodies (alpha-N46PA) in patients with pSS, SLE, RA and normal controls were 78.5%, 10.4%, 21.6% and 6.0%, respectively. The sensitivity and specificity of the autoantibodies in pSS were 78.5% and 86.8%, respectively. These results suggest the alpha-N46PA which shows highest sensitivity and specificity is of significance to develop an effective diagnostic approach for pSS. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/17614976/The_epitope_study_of_alpha_fodrin_autoantibody_in_primary_Sjögren's_syndrome_ L2 - https://doi.org/10.1111/j.1365-2249.2007.03435.x DB - PRIME DP - Unbound Medicine ER -