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Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.
Appl Environ Microbiol 2007; 73(17):5660-2AE

Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

Authors+Show Affiliations

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Panagiotis.Karanis@uk-koeln.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17616628

Citation

Karanis, Panagiotis, et al. "Development and Preliminary Evaluation of a Loop-mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples." Applied and Environmental Microbiology, vol. 73, no. 17, 2007, pp. 5660-2.
Karanis P, Thekisoe O, Kiouptsi K, et al. Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples. Appl Environ Microbiol. 2007;73(17):5660-2.
Karanis, P., Thekisoe, O., Kiouptsi, K., Ongerth, J., Igarashi, I., & Inoue, N. (2007). Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples. Applied and Environmental Microbiology, 73(17), pp. 5660-2.
Karanis P, et al. Development and Preliminary Evaluation of a Loop-mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples. Appl Environ Microbiol. 2007;73(17):5660-2. PubMed PMID: 17616628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples. AU - Karanis,Panagiotis, AU - Thekisoe,Oriel, AU - Kiouptsi,Klytaimnistra, AU - Ongerth,Jerry, AU - Igarashi,Ikuo, AU - Inoue,Noborou, Y1 - 2007/07/06/ PY - 2007/7/10/pubmed PY - 2007/10/24/medline PY - 2007/7/10/entrez SP - 5660 EP - 2 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 73 IS - 17 N2 - A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/17616628/Development_and_preliminary_evaluation_of_a_loop_mediated_isothermal_amplification_procedure_for_sensitive_detection_of_cryptosporidium_oocysts_in_fecal_and_water_samples_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=17616628 DB - PRIME DP - Unbound Medicine ER -