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Inconsistent responses of cytomegalovirus-specific T cells to pp65 and IE-1 versus infected dendritic cells in organ transplant recipients.
Am J Transplant. 2007 Aug; 7(8):1997-2005.AJ

Abstract

CD4(+) and CD8(+) T cells specific for human cytomegalovirus (HCMV) and two immunodominant HCMV antigens (pp65 and IE-1) were monitored in 20 solid organ transplant recipients undergoing primary (n = 4) or reactivated (n = 16) HCMV infection during the first year after transplantation by using as a stimulator either HCMV-infected autologous dendritic cells (DCs) or pp65- or IE-1 peptide mixtures. Turnaround times for test performance were 7 days for infected DCs and 24 h for peptides. Using infected DCs, HCMV-specific T-cell restoration occurred in all patients for CD8(+) and in 18/20 (90%) for CD4(+) T-cell subpopulations, resulting in virus clearance from blood. Using peptide mixtures, T-cell responses were less frequently detected. In detail, 14 (70%) patients showed pp65-specific CD8(+) T cells and 10 (50%) patients IE-1-specific CD8(+) T cells, whereas pp65-specific CD4(+) T cells were detected in 14 (70%) patients, and IE-1-specific CD4(+) T cells in three (15%) patients only. Protection from HCMV infection was associated with the presence of a HCMV-specific T-cell response directed against multiple viral proteins, but not against pp65 or IE-1 only. In conclusion, the use of pp65 and IE-1 peptide mixtures for rapid monitoring of HCMV-specific T-cell responses in solid organ transplant recipients underestimates the actual T-cell immune response against HCMV.

Authors+Show Affiliations

Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17617865

Citation

Lilleri, D, et al. "Inconsistent Responses of Cytomegalovirus-specific T Cells to Pp65 and IE-1 Versus Infected Dendritic Cells in Organ Transplant Recipients." American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, vol. 7, no. 8, 2007, pp. 1997-2005.
Lilleri D, Zelini P, Fornara C, et al. Inconsistent responses of cytomegalovirus-specific T cells to pp65 and IE-1 versus infected dendritic cells in organ transplant recipients. Am J Transplant. 2007;7(8):1997-2005.
Lilleri, D., Zelini, P., Fornara, C., Comolli, G., & Gerna, G. (2007). Inconsistent responses of cytomegalovirus-specific T cells to pp65 and IE-1 versus infected dendritic cells in organ transplant recipients. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 7(8), 1997-2005.
Lilleri D, et al. Inconsistent Responses of Cytomegalovirus-specific T Cells to Pp65 and IE-1 Versus Infected Dendritic Cells in Organ Transplant Recipients. Am J Transplant. 2007;7(8):1997-2005. PubMed PMID: 17617865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inconsistent responses of cytomegalovirus-specific T cells to pp65 and IE-1 versus infected dendritic cells in organ transplant recipients. AU - Lilleri,D, AU - Zelini,P, AU - Fornara,C, AU - Comolli,G, AU - Gerna,G, PY - 2007/7/10/pubmed PY - 2007/9/26/medline PY - 2007/7/10/entrez SP - 1997 EP - 2005 JF - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JO - Am J Transplant VL - 7 IS - 8 N2 - CD4(+) and CD8(+) T cells specific for human cytomegalovirus (HCMV) and two immunodominant HCMV antigens (pp65 and IE-1) were monitored in 20 solid organ transplant recipients undergoing primary (n = 4) or reactivated (n = 16) HCMV infection during the first year after transplantation by using as a stimulator either HCMV-infected autologous dendritic cells (DCs) or pp65- or IE-1 peptide mixtures. Turnaround times for test performance were 7 days for infected DCs and 24 h for peptides. Using infected DCs, HCMV-specific T-cell restoration occurred in all patients for CD8(+) and in 18/20 (90%) for CD4(+) T-cell subpopulations, resulting in virus clearance from blood. Using peptide mixtures, T-cell responses were less frequently detected. In detail, 14 (70%) patients showed pp65-specific CD8(+) T cells and 10 (50%) patients IE-1-specific CD8(+) T cells, whereas pp65-specific CD4(+) T cells were detected in 14 (70%) patients, and IE-1-specific CD4(+) T cells in three (15%) patients only. Protection from HCMV infection was associated with the presence of a HCMV-specific T-cell response directed against multiple viral proteins, but not against pp65 or IE-1 only. In conclusion, the use of pp65 and IE-1 peptide mixtures for rapid monitoring of HCMV-specific T-cell responses in solid organ transplant recipients underestimates the actual T-cell immune response against HCMV. SN - 1600-6135 UR - https://www.unboundmedicine.com/medline/citation/17617865/Inconsistent_responses_of_cytomegalovirus_specific_T_cells_to_pp65_and_IE_1_versus_infected_dendritic_cells_in_organ_transplant_recipients_ L2 - https://doi.org/10.1111/j.1600-6143.2007.01890.x DB - PRIME DP - Unbound Medicine ER -