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Pyrrolidine dithiocarbamate prevents 60 minutes of warm mesenteric ischemia/reperfusion injury in rats.
Am J Surg. 2007 Aug; 194(2):255-62.AJ

Abstract

BACKGROUND

Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight thiol antioxidant and potent inhibitor of nuclear factor-kappaB (NF-kappaB) activation. It has been shown to attenuate harmful effects of ischemia/reperfusion (I/R) injury in many organs. In recent animal studies, destructive effects of reperfusion injury has been demonstrated. In this study, we aimed to investigate whether PDTC prevents harmful effects of superior mesenteric I/R injury in rats.

METHODS

Wistar-albino rats were randomly allocated into the following 4 groups: (1) sham-operated group--these animals underwent laparotomy without I/R injury (group I, n = 12); (2) sham+PDTC group--identical to sham-operated rats except for the administration of PDTC (100 mg/kg intravenous bolus) 30 minutes prior to the commencement of the experimental period (group II, n = 12); (3) I/R group--these animals underwent laparotomy and 60 minutes of ischemia followed by 120 minutes of reperfusion (group III, n = 12); (4) PDTC-treated group (100 mg/kg, intravenously, before the I/R, group IV, n = 12). All animals were killed, and intestinal tissue samples were obtained for investigation of intestinal mucosal injury, myeloperoxidase (MPO) activity, malondialdehyde (MDA) levels, glutathione (GSH) levels, and intestinal edema.

RESULTS

There was a statistically significant decrease in GSH levels, along with an increase in intestinal mucosal injury scores, MPO activity, MDA levels, and intestinal tissue wet-to-dry weight ratios in group III when compared to groups I, II, and IV (P < .05). However, PDTC treatment led to a statistically significant increase in GSH levels, along with a decrease in intestinal mucosal injury scores, MPO activity, MDA levels, and intestinal tissue wet-to-dry weight ratios in group IV (P < .05).

CONCLUSIONS

This study showed that PDTC treatment significantly prevented the reperfusion injury caused by superior mesenteric I/R. Further clinical studies are needed to clarify whether PDTC may be a useful therapeutic agent to use in particular operations where the reperfusion injury occurs.

Authors+Show Affiliations

Faculty of Medicine, Department of General Surgery, Pamukkale University, 20070 Denizli, Turkey. zteke_md@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17618816

Citation

Teke, Zafer, et al. "Pyrrolidine Dithiocarbamate Prevents 60 Minutes of Warm Mesenteric Ischemia/reperfusion Injury in Rats." American Journal of Surgery, vol. 194, no. 2, 2007, pp. 255-62.
Teke Z, Kabay B, Aytekin FO, et al. Pyrrolidine dithiocarbamate prevents 60 minutes of warm mesenteric ischemia/reperfusion injury in rats. Am J Surg. 2007;194(2):255-62.
Teke, Z., Kabay, B., Aytekin, F. O., Yenisey, C., Demirkan, N. C., Sacar, M., Erdem, E., & Ozden, A. (2007). Pyrrolidine dithiocarbamate prevents 60 minutes of warm mesenteric ischemia/reperfusion injury in rats. American Journal of Surgery, 194(2), 255-62.
Teke Z, et al. Pyrrolidine Dithiocarbamate Prevents 60 Minutes of Warm Mesenteric Ischemia/reperfusion Injury in Rats. Am J Surg. 2007;194(2):255-62. PubMed PMID: 17618816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pyrrolidine dithiocarbamate prevents 60 minutes of warm mesenteric ischemia/reperfusion injury in rats. AU - Teke,Zafer, AU - Kabay,Burhan, AU - Aytekin,Faruk Onder, AU - Yenisey,Cigdem, AU - Demirkan,Nese Calli, AU - Sacar,Mustafa, AU - Erdem,Ergun, AU - Ozden,Akin, PY - 2006/03/20/received PY - 2006/06/16/revised PY - 2006/06/16/accepted PY - 2007/7/10/pubmed PY - 2007/8/1/medline PY - 2007/7/10/entrez SP - 255 EP - 62 JF - American journal of surgery JO - Am J Surg VL - 194 IS - 2 N2 - BACKGROUND: Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight thiol antioxidant and potent inhibitor of nuclear factor-kappaB (NF-kappaB) activation. It has been shown to attenuate harmful effects of ischemia/reperfusion (I/R) injury in many organs. In recent animal studies, destructive effects of reperfusion injury has been demonstrated. In this study, we aimed to investigate whether PDTC prevents harmful effects of superior mesenteric I/R injury in rats. METHODS: Wistar-albino rats were randomly allocated into the following 4 groups: (1) sham-operated group--these animals underwent laparotomy without I/R injury (group I, n = 12); (2) sham+PDTC group--identical to sham-operated rats except for the administration of PDTC (100 mg/kg intravenous bolus) 30 minutes prior to the commencement of the experimental period (group II, n = 12); (3) I/R group--these animals underwent laparotomy and 60 minutes of ischemia followed by 120 minutes of reperfusion (group III, n = 12); (4) PDTC-treated group (100 mg/kg, intravenously, before the I/R, group IV, n = 12). All animals were killed, and intestinal tissue samples were obtained for investigation of intestinal mucosal injury, myeloperoxidase (MPO) activity, malondialdehyde (MDA) levels, glutathione (GSH) levels, and intestinal edema. RESULTS: There was a statistically significant decrease in GSH levels, along with an increase in intestinal mucosal injury scores, MPO activity, MDA levels, and intestinal tissue wet-to-dry weight ratios in group III when compared to groups I, II, and IV (P < .05). However, PDTC treatment led to a statistically significant increase in GSH levels, along with a decrease in intestinal mucosal injury scores, MPO activity, MDA levels, and intestinal tissue wet-to-dry weight ratios in group IV (P < .05). CONCLUSIONS: This study showed that PDTC treatment significantly prevented the reperfusion injury caused by superior mesenteric I/R. Further clinical studies are needed to clarify whether PDTC may be a useful therapeutic agent to use in particular operations where the reperfusion injury occurs. SN - 1879-1883 UR - https://www.unboundmedicine.com/medline/citation/17618816/Pyrrolidine_dithiocarbamate_prevents_60_minutes_of_warm_mesenteric_ischemia/reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9610(07)00336-4 DB - PRIME DP - Unbound Medicine ER -