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Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 01; 856(1-2):245-51.JC

Abstract

A simple, rapid and sensitive method was developed for routine analysis of riboflavin in beverage, green tea and urine by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection (LIF). The difference between the present detector in the study and others is that an optical fiber was adopted in the former, which can guide the excitation light into the capillary right at the detection window. The linearity of the method (r(2)=0.998) was good over the concentration range from 0.05 to 20 microM for riboflavin. The limit of detection (LOD) was determined using linear regression analysis and was found to be 3.0 nM. The percent recoveries of riboflavin in beverage, green tea and urine samples were 95.3+/-2.9, 105.5+/-3.9 and 94.3+/-1.7, respectively. These results of quantitative analysis of riboflavin in beverage and green tea samples is in agreement with that of obtained by the AOAC of fluorometric method. In the analysis of urine samples, all electropherograms of urine samples and corresponding concentrations of riboflavin in the period of 13 h after orally administrating the ingestion of vitamin B(2) tablets were illustrated.

Authors+Show Affiliations

College of Chemistry, Sichuan University, Chengdu 610065, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17625992

Citation

Hu, Li, et al. "Determination of Riboflavin in Urine and Beverages By Capillary Electrophoresis With In-column Optical Fiber Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 856, no. 1-2, 2007, pp. 245-51.
Hu L, Yang X, Wang C, et al. Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;856(1-2):245-51.
Hu, L., Yang, X., Wang, C., Yuan, H., & Xiao, D. (2007). Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 856(1-2), 245-51.
Hu L, et al. Determination of Riboflavin in Urine and Beverages By Capillary Electrophoresis With In-column Optical Fiber Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):245-51. PubMed PMID: 17625992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection. AU - Hu,Li, AU - Yang,Xiupei, AU - Wang,Chunling, AU - Yuan,Hongyan, AU - Xiao,Dan, Y1 - 2007/06/22/ PY - 2006/12/18/received PY - 2007/05/28/revised PY - 2007/06/12/accepted PY - 2007/7/13/pubmed PY - 2007/12/6/medline PY - 2007/7/13/entrez SP - 245 EP - 51 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 856 IS - 1-2 N2 - A simple, rapid and sensitive method was developed for routine analysis of riboflavin in beverage, green tea and urine by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection (LIF). The difference between the present detector in the study and others is that an optical fiber was adopted in the former, which can guide the excitation light into the capillary right at the detection window. The linearity of the method (r(2)=0.998) was good over the concentration range from 0.05 to 20 microM for riboflavin. The limit of detection (LOD) was determined using linear regression analysis and was found to be 3.0 nM. The percent recoveries of riboflavin in beverage, green tea and urine samples were 95.3+/-2.9, 105.5+/-3.9 and 94.3+/-1.7, respectively. These results of quantitative analysis of riboflavin in beverage and green tea samples is in agreement with that of obtained by the AOAC of fluorometric method. In the analysis of urine samples, all electropherograms of urine samples and corresponding concentrations of riboflavin in the period of 13 h after orally administrating the ingestion of vitamin B(2) tablets were illustrated. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17625992/Determination_of_riboflavin_in_urine_and_beverages_by_capillary_electrophoresis_with_in_column_optical_fiber_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00439-4 DB - PRIME DP - Unbound Medicine ER -