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Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica.
Antivir Chem Chemother. 2007; 18(3):153-62.AC

Abstract

Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of alpha-(1 --> 3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1 --> 3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.

Links

Publisher Full Text
journals.sagepub.com
journals.sagepub.com

Authors+Show Affiliations

Mandal P
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, WB, India.
Mateu CG
No affiliation info available
Chattopadhyay K
No affiliation info available
Pujol CA
No affiliation info available
Damonte EB
No affiliation info available
Ray B
No affiliation info available

MeSH

AnimalsAntiviral AgentsCell ExtractsChlorocebus aethiopsChromatography, Ion ExchangeGalactoseHerpesvirus 1, HumanHerpesvirus 2, HumanMolecular WeightPhaeophytaPolysaccharidesSpectrum AnalysisVero CellsVirus AttachmentVirus InactivationXylose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17626599

Citation

Mandal, Pinaki, et al. "Structural Features and Antiviral Activity of Sulphated Fucans From the Brown Seaweed Cystoseira Indica." Antiviral Chemistry & Chemotherapy, vol. 18, no. 3, 2007, pp. 153-62.
Mandal P, Mateu CG, Chattopadhyay K, et al. Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica. Antivir Chem Chemother. 2007;18(3):153-62.
Mandal, P., Mateu, C. G., Chattopadhyay, K., Pujol, C. A., Damonte, E. B., & Ray, B. (2007). Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica. Antiviral Chemistry & Chemotherapy, 18(3), 153-62.
Mandal P, et al. Structural Features and Antiviral Activity of Sulphated Fucans From the Brown Seaweed Cystoseira Indica. Antivir Chem Chemother. 2007;18(3):153-62. PubMed PMID: 17626599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica. AU - Mandal,Pinaki, AU - Mateu,Cecilia Gabriela, AU - Chattopadhyay,Kausik, AU - Pujol,Carlos Alberto, AU - Damonte,Elsa Beatriz, AU - Ray,Bimalendu, PY - 2007/7/14/pubmed PY - 2007/9/22/medline PY - 2007/7/14/entrez SP - 153 EP - 62 JF - Antiviral chemistry & chemotherapy JO - Antivir Chem Chemother VL - 18 IS - 3 N2 - Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of alpha-(1 --> 3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1 --> 3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer. SN - 0956-3202 UR - https://www.unboundmedicine.com/medline/citation/17626599/Structural_features_and_antiviral_activity_of_sulphated_fucans_from_the_brown_seaweed_Cystoseira_indica_ L2 - https://journals.sagepub.com/doi/10.1177/095632020701800305?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -